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The map label for this gene is rfaE

Identifier: 218696756

GI number: 218696756

Start: 3544071

End: 3545504

Strand: Reverse

Name: rfaE

Synonym: EC55989_3466

Alternate gene names: 218696756

Gene position: 3545504-3544071 (Counterclockwise)

Preceding gene: 218696757

Following gene: 218696753

Centisome position: 68.78

GC content: 56.35

Gene sequence:

>1434_bases
ATGAAAGTAACGCTGCCAGAGTTTGAACGTGCAGGAGTGATGGTGGTTGGTGATGTGATGCTGGATCGTTACTGGTACGG
TCCCACCAGCCGTATCTCGCCGGAAGCGCCGGTGCCCGTGGTTAAAGTCAATACCATCGAAGAACGTCCGGGCGGCGCGG
CTAACGTGGCAATGAACATCGCTTCTCTCGGTGCCAATGCTCGCCTGGTCGGGCTGACGGGCATTGACGATGCGGCGCGT
GCGCTGAGTAAATCTCTGGCCGACGTCAACGTCAAATGCGACTTCGTTTCTGTACCGACGCATCCGACTATCACCAAATT
ACGGGTACTTTCCCGCAACCAACAGCTGATCCGCCTGGATTTTGAAGAAGGTTTCGAAGGTGTTGATCCGCAGCCGCTGC
ACGAGCGGATTAATCAGGCGCTGAGTTCGATTGGCGCGCTGGTGCTTTCTGACTACGCCAAAGGTGCGCTGGCAAGCGTA
CAGCAGATGATCCAACTGGCGCGTAAAGCGGGCGTTCCGGTGCTGATTGATCCAAAAGGTACCGATTTTGAGCGCTACCG
CGGCGCTACGCTGTTAACGCCGAATCTCTCGGAATTTGAAGCTGTTGTCGGTAAATGTAAGACCGAAGAAGAGATTGTTG
AGCGCGGCATGAAACTGATTGCCGATTACGAACTCTCGGCTCTGTTAGTGACCCGTTCCGAACAGGGTATGTCGCTGCTG
CAACCGGGTAAAGCGCCGCTGCATATGCCAACCCAGGCGCAGGAAGTGTATGACGTTACCGGTGCGGGCGACACGGTGAT
TGGCGTCCTGGCGGCAACGCTGGCAGCGGGTAATTCGCTGGAAGAAGCCTGCTTCTTTGCCAATGCGGCGGCTGGCGTGG
TGGTCGGCAAACTGGGAACCTCCACGGTTTCGCCGATCGAGCTGGAAAATGCAGTGCGTGGGCGCGCAGATACCGGCTTT
GGTGTGATGACCGAAGAGGAACTGAAGCTTGCTGTAGCGGCAGCGCGTAAACGCGGCGAAAAGGTGGTAATGACCAACGG
TGTCTTTGACATCCTGCACGCCGGGCACGTCTCCTATCTGGCAAATGCTCGCAAACTGGGTGACCGTCTGATTGTCGCTG
TGAACAGCGATGCCTCCACCAAACGGCTGAAAGGGGATTCCCGCCCGGTAAACCCACTCGAGCAGCGTATGATTGTGCTG
GGCGCACTGGAAGCGGTCGACTGGGTAGTGTCGTTTGAAGAAGACACGCCGCAGCGTCTGATCGCCGGGATCCTGCCAGA
TCTGCTGGTGAAAGGCGGCGACTATAAACCAGAAGAAATTGCCGGGAGTAAAGAAGTCTGGGCCAACGGCGGCGAAGTGC
TGGTGCTCAACTTCGAAGACGGTTGCTCGACCACTAACATCATCAAGAAGATCCAACAGGATAAAAAAGGCTAA

Upstream 100 bases:

>100_bases
CAGAGCGTGAACTGGTGCGGGCAAGCTGGCAGAAGTGGCTGGTGGAAGAATGAAGTATGGTATTATCGCGCGCAAATTTT
GAATCTCTCAGGAGACAGGA

Downstream 100 bases:

>100_bases
CCGGAAAGCGGTTCACAGATCTCGCTTCGTAGCAGGCAGGCCTATTGACGCTGTTGTTTTTTCAACCTAAAGTAAAGGAA
CAAGGGTAAGGGAGGATTTC

Product: bifunctional heptose 7-phosphate kinase/heptose 1-phosphate adenyltransferase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVNTIEERPGGAANVAMNIASLGANARLVGLTGIDDAAR
ALSKSLADVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMKLIADYELSALLVTRSEQGMSLL
QPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAAGNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGF
GVMTEEELKLAVAAARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNPLEQRMIVL
GALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANGGEVLVLNFEDGCSTTNIIKKIQQDKKG

Sequences:

>Translated_477_residues
MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVNTIEERPGGAANVAMNIASLGANARLVGLTGIDDAAR
ALSKSLADVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMKLIADYELSALLVTRSEQGMSLL
QPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAAGNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGF
GVMTEEELKLAVAAARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNPLEQRMIVL
GALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANGGEVLVLNFEDGCSTTNIIKKIQQDKKG
>Mature_477_residues
MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVNTIEERPGGAANVAMNIASLGANARLVGLTGIDDAAR
ALSKSLADVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMKLIADYELSALLVTRSEQGMSLL
QPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAAGNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGF
GVMTEEELKLAVAAARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNPLEQRMIVL
GALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANGGEVLVLNFEDGCSTTNIIKKIQQDKKG

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=477, Percent_Identity=100, Blast_Score=967, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 51051; Mature: 51051

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVNTIEERPGGAANVAMNI
CCCCCCCHHHCCEEEEEHHHHHHEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHH
ASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLD
HHCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHCCCCEEEEEE
FEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASVQQMIQLARKAGVPVLIDPKG
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
TDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMKLIADYELSALLVTRSEQGMSLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHH
QPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAAGNSLEEACFFANAAAGVVVGKLGT
CCCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCC
STVSPIELENAVRGRADTGFGVMTEEELKLAVAAARKRGEKVVMTNGVFDILHAGHVSYL
CCCCCEEHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
ANARKLGDRLIVAVNSDASTKRLKGDSRPVNPLEQRMIVLGALEAVDWVVSFEEDTPQRL
HHHHHCCCEEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHH
IAGILPDLLVKGGDYKPEEIAGSKEVWANGGEVLVLNFEDGCSTTNIIKKIQQDKKG
HHHHHHHHHHCCCCCCHHHHCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVNTIEERPGGAANVAMNI
CCCCCCCHHHCCEEEEEHHHHHHEECCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHH
ASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHPTITKLRVLSRNQQLIRLD
HHCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEEEECCCCCCHHHHHHHCCCCEEEEEE
FEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASVQQMIQLARKAGVPVLIDPKG
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCC
TDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMKLIADYELSALLVTRSEQGMSLL
CCHHHHCCCEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHH
QPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAAGNSLEEACFFANAAAGVVVGKLGT
CCCCCCCCCCCCHHHHEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEECCCC
STVSPIELENAVRGRADTGFGVMTEEELKLAVAAARKRGEKVVMTNGVFDILHAGHVSYL
CCCCCEEHHHHHCCCCCCCCCCEEHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHH
ANARKLGDRLIVAVNSDASTKRLKGDSRPVNPLEQRMIVLGALEAVDWVVSFEEDTPQRL
HHHHHCCCEEEEEECCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCCHHHH
IAGILPDLLVKGGDYKPEEIAGSKEVWANGGEVLVLNFEDGCSTTNIIKKIQQDKKG
HHHHHHHHHHCCCCCCHHHHCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA