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The map label for this gene is glcD

Identifier: 218696684

GI number: 218696684

Start: 3474380

End: 3475879

Strand: Reverse

Name: glcD

Synonym: EC55989_3389

Alternate gene names: 218696684

Gene position: 3475879-3474380 (Counterclockwise)

Preceding gene: 218696685

Following gene: 218696683

Centisome position: 67.43

GC content: 56.53

Gene sequence:

>1500_bases
ATGAGCATCTTGTACGAAGAGCGTCTTGATGGCGCTTTACCCGATGTCGACCGCACATCGGTACTGATGGCACTGCGTGA
GCATGTCCCTGGACTTGAGATCCTGCATACCGATGAGGAGATCATTCCTTACGAGTGTGACGGGTTGAGCGCGTATCGCA
CGCGTCCATTACTGGTTGTTCTGCCTAAGCAAATGGAACAGGTGACAGCGATTCTGGCTGTCTGCCATCGCCTGCGTGTA
CCGGTGGTGACCCGTGGTGCAGGCACCGGGCTTTCTGGTGGCGCGCTGCCGCTGGAAAAAGGTGTGTTGTTGGTGATGGC
GCGCTTTAAAGAGATCCTCGACATTAACCCCGTTGGTCGCCGCGCGCGCGTGCAGCCTGGAGTGCGTAACCTGGCGATCT
CCCAGGCCGTTGCGCCGCATAATCTCTACTACGCACCGGACCCTTCCTCACAAATAGCCTGTTCAATTGGCGGCAATGTG
GCGGAAAATGCCGGTGGCGTCCACTGCCTGAAATATGGTCTGACCGTACATAACCTGCTGAAAATTGAAGTGCAAACGCT
GGACGGCGAGGCGCTGACGCTGGGATCGGACGCGCTGGATTCACCTGGTTTTGACCTGCTGGCGCTGTTCACCGGATCGG
AAGGTATGCTCGGCGTGACCACCGAAGTGACGGTAAAACTGCTGCCGAAGCCGCCCGTGGCGCGGGTTCTGTTAGCCAGC
TTTGACTCGGTAGAAAAAGCCGGACTTGCAGTTGGTGACATCATCGCCAATGGCATTATCCCCGGCGGGCTGGAGATGAT
GGATAACCTGTCGATCCGCGCGGCGGAAGATTTTATTCATGCCGGTTATCCCGTCGACGCCGAAGCGATTTTGTTATGCG
AGCTGGACGGCGTGGAGTCTGACGTACAGGAAGACTGCGAGCGGGTTAACGACATCTTGTTGAAAGCGGGCGCGACTGAC
GTCCGTCTGGCACAGGACGAAGCAGAGCGCGTACGTTTCTGGGCCGGTCGCAAAAATGCGTTCCCGGCGGTAGGACGTAT
CTCCCCGGATTACTACTGCATGGATGGCACCATCCCGCGTCGCGCCCTGCCTGGCGTACTGGAAGGCATTGCCCGTTTAT
CGCAGCAATATGATTTACGTGTTGCCAACGTCTTTCATGCCGGAGATGGCAACATGCACCCGTTAATCCTTTTCGATGCC
AACGAACCCGGTGAATTTGCCCGCGCGGAAGAGCTGGGTGGGAAGATCCTCGAACTCTGCGTTGAAGTTGGCGGCAGCAT
CAGTGGGGAACATGGCATCGGGCGAGAAAAAATCAATCAAATGTGCGCCCAGTTCAACAGCGATGAAATCACGACCTTCC
ATGCGGTCAAGGCGGCGTTTGACCCCGATGGTTTGCTGAACCCTGGGAAAAACATTCCCACGCTACACCGCTGTGCTGAA
TTTGGTGCCATGCATGTGCATCACGGTCATTTACCTTTCCCTGAACTGGAGCGTTTCTGA

Upstream 100 bases:

>100_bases
TAAAGAGATAGACGAAAACGAAAAGCCCGCTTAATAACTGTTCACAGAAGCAGCGCGCAAAAATCAGCTGCCACACAACA
CAACAAAGCGAAGCCTACTC

Downstream 100 bases:

>100_bases
TGCTACGCGAGTGTGATTACAGCCAGGCGCTGCTGGAGCAGGTGAATCAGGCAATTAGCGATAAAACGCCGCTGGTGATT
CAGGGCAGCAATAGCAAAGC

Product: glycolate oxidase subunit GlcD

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRV
PVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLAS
FDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATD
VRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDA
NEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAE
FGAMHVHHGHLPFPELERF

Sequences:

>Translated_499_residues
MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRV
PVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLAS
FDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATD
VRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDA
NEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAE
FGAMHVHHGHLPFPELERF
>Mature_498_residues
SILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVP
VVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVA
ENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLASF
DSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDV
RLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDAN
EPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAEF
GAMHVHHGHLPFPELERF

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Homo sapiens, GI37595756, Length=425, Percent_Identity=30.5882352941176, Blast_Score=192, Evalue=8e-49,
Organism=Homo sapiens, GI37595754, Length=448, Percent_Identity=30.1339285714286, Blast_Score=185, Evalue=7e-47,
Organism=Homo sapiens, GI119964728, Length=469, Percent_Identity=28.7846481876333, Blast_Score=156, Evalue=5e-38,
Organism=Escherichia coli, GI1789351, Length=499, Percent_Identity=100, Blast_Score=1011, Evalue=0.0,
Organism=Escherichia coli, GI1787977, Length=503, Percent_Identity=24.0556660039761, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71992373, Length=431, Percent_Identity=28.3062645011601, Blast_Score=177, Evalue=8e-45,
Organism=Caenorhabditis elegans, GI71992381, Length=427, Percent_Identity=26.463700234192, Blast_Score=157, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI71992392, Length=359, Percent_Identity=27.2980501392758, Blast_Score=147, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17534361, Length=471, Percent_Identity=28.2377919320594, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17556096, Length=453, Percent_Identity=21.1920529801325, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6320023, Length=454, Percent_Identity=29.295154185022, Blast_Score=173, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6320764, Length=475, Percent_Identity=27.3684210526316, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320027, Length=436, Percent_Identity=25.9174311926606, Blast_Score=155, Evalue=1e-38,
Organism=Drosophila melanogaster, GI18921117, Length=444, Percent_Identity=28.3783783783784, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24639277, Length=444, Percent_Identity=28.3783783783784, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24639275, Length=444, Percent_Identity=28.3783783783784, Blast_Score=137, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24653753, Length=222, Percent_Identity=25.2252252252252, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLCD_ECOL6 (P0AEQ0)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_755580.1
- ProteinModelPortal:   P0AEQ0
- SMR:   P0AEQ0
- EnsemblBacteria:   EBESCT00000042440
- GeneID:   1039751
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c3709
- GeneTree:   EBGT00050000008853
- HOGENOM:   HBG553036
- OMA:   QLPFPEL
- ProtClustDB:   PRK11230
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.30.465.20
- Gene3D:   G3DSA:1.10.45.10
- TIGRFAMs:   TIGR00387

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4; SSF55103 FAD-binding_2; SSF56176 FAD-binding_2

EC number: 1.1.3.15

Molecular weight: Translated: 53812; Mature: 53681

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS51387 FAD_PCMH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVV
CCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCHHCCCCEEEE
LPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGR
CCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCC
RARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL
CCCCCCCHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCEEEEE
KIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLAS
EEEEEEECCCEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHH
FDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVES
HHHHHHHCCHHHHHHHHCCCCCCHHHHHCCCEEEHHHHHHCCCCCCCCEEEEEECCCCCH
DVQEDCERVNDILLKAGATDVRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPR
HHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCH
RALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEPGEFARAEELGGKILELC
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
VEVGGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAE
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHH
FGAMHVHHGHLPFPELERF
HCCEEEECCCCCCHHHHCC
>Mature Secondary Structure 
SILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPYECDGLSAYRTRPLLVV
CCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCEECCCCCHHCCCCEEEE
LPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGVLLVMARFKEILDINPVGR
CCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCCC
RARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNVAENAGGVHCLKYGLTVHNLL
CCCCCCCHHHHHHHHCCCCCCCEECCCCCCCEEEEECCCCCCCCCCEEEEEECCCEEEEE
KIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLGVTTEVTVKLLPKPPVARVLLAS
EEEEEEECCCEEEECCCCCCCCCCEEEEEEECCCCCEEEEEEEEEEECCCCHHHHHHHHH
FDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAEDFIHAGYPVDAEAILLCELDGVES
HHHHHHHCCHHHHHHHHCCCCCCHHHHHCCCEEEHHHHHHCCCCCCCCEEEEEECCCCCH
DVQEDCERVNDILLKAGATDVRLAQDEAERVRFWAGRKNAFPAVGRISPDYYCMDGTIPR
HHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCH
RALPGVLEGIARLSQQYDLRVANVFHAGDGNMHPLILFDANEPGEFARAEELGGKILELC
HHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
VEVGGSISGEHGIGREKINQMCAQFNSDEITTFHAVKAAFDPDGLLNPGKNIPTLHRCAE
HHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCHHHHHHH
FGAMHVHHGHLPFPELERF
HCCEEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157