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The map label for this gene is insI

Identifier: 218696576

GI number: 218696576

Start: 3355072

End: 3356223

Strand: Reverse

Name: insI

Synonym: EC55989_3272

Alternate gene names: 218696576

Gene position: 3356223-3355072 (Counterclockwise)

Preceding gene: 218696578

Following gene: 218696568

Centisome position: 65.11

GC content: 46.01

Gene sequence:

>1152_bases
ATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGC
GAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACTGGCGGCATAAAACCCCATGAGCGTAAGC
GGGCTGTAGCTCACCTGACACTGTCTGAGCGCGAGGAGATACGAGCTGGTTTGTCAGCCAAAATGAGCATTCGTGCGATA
GCTACTGCGCTGAATCGCAGTCCTTCGACGATCTCACGTGAAGTTCAGCGTAATCGGGGCAGACGCTATTACAAAGCTGT
TGATGCTAATAACCGAGCCAACAGAATGGCGAAAAGGCCAAAACCGTGCTTACTGGATCAAAATTTGCCATTGCGAAAGC
TTGTTCTGGAAAAGCTGGAGATGAAATGGTCTCCAGAGCAAATATCAGGATGGTTAAGGCGAACAAAACCACGTCAAAAA
ACGCTGCGAATATCACCTGAGACAATTTATAAAACGCTGTACTTTCGTAGCCGTGAAGCGCTACACCACCTGAATATACA
GCATCTGCGACGGTCGCATAGCCTTCGCCATGGCAGGCGTCATACCCGCAAAGGCGAAAGAGGTACGATTAACATAGTGA
ACGGAACACCAATTCACGAACGTTCCCGAAATATCGATAACAGACGCTCTCTGGGGCATTGGGAGGGCGATTTAGTCTCA
GGTACAAAAAACTCTCATATAGCCACACTTGTAGACCGAAAATCACGTTATACGATCATCCTTAGACTCAGGGGCAAAGA
TTCTGTCTCAGTAAATCAGGCTCTTACCGACAAATTCCTGAGTTTACCGTCAGAACTCAGAAAATCACTGACATGGGACA
GAGGAATGGAACTGGCCAGACATCTAGAATTTACTGTCAGCACCGGCGTTAAAGTTTACTTCTGCGATCCTCAGAGTCCT
TGGCAGCGGGGAACAAATGAGAACACAAATGGGCTAATTCGGCAGTACTTTCCTAAAAAGACATGTCTTGCCCAATATAC
TCAACATGAACTAGATCTGGTTGCTGCTCAGCTAAACAACAGACCGAGAAAGACACTGAAGTTCAAAACACCGAAAGAGA
TAATTGAAAGGGGTGTTGCATTGACAGATTGA

Upstream 100 bases:

>100_bases
CGAGAACTGTTCAGGGAGTCTGACATCAAGAGCGCGGTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATG
GGGTTGCGGAGGTTTTTTGA

Downstream 100 bases:

>100_bases
ATCTACAGTAGCCTTTTTTAATATTTCATTCTCCATTTCAATGCGTTGTAGCTTTTTCCTCAGCTTACGTATTTCGATTT
GTTCTGGTGTTATCGGAGAG

Product: IS30 transposase; KpLE2 phage-like element

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 383; Mature: 383

Protein sequence:

>383_residues
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAI
ATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
GTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSP
WQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD

Sequences:

>Translated_383_residues
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAI
ATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
GTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSP
WQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD
>Mature_383_residues
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAI
ATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
GTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALTDKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSP
WQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1790736, Length=383, Percent_Identity=100, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI1787671, Length=383, Percent_Identity=100, Blast_Score=788, Evalue=0.0,
Organism=Escherichia coli, GI1786450, Length=383, Percent_Identity=99.7389033942559, Blast_Score=787, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 44282; Mature: 44282

Theoretical pI: Translated: 11.48; Mature: 11.48

Prosite motif: PS50994 INTEGRASE ; PS01043 TRANSPOSASE_IS30

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLT
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHEE
LSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRP
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCC
KPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQKTLRISPETIYKTLYFRSREA
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHEEECCHHHHHHHHHHHHHHH
LHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
HHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCHHHCCCCCCCCCCC
GTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALTDKFLSLPSELRKSLTWDRGMELAR
CCCCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHH
HLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNN
HHHHEEECCCEEEEECCCCHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
RPRKTLKFKTPKEIIERGVALTD
CCCCCEECCCHHHHHHCCCCCCC
>Mature Secondary Structure
MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDTGGIKPHERKRAVAHLT
CCCCCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHEE
LSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRRYYKAVDANNRANRMAKRP
HHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCC
KPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQKTLRISPETIYKTLYFRSREA
CCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHEEECCHHHHHHHHHHHHHHH
LHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPIHERSRNIDNRRSLGHWEGDLVS
HHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCHHHCCCCCCCCCCC
GTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALTDKFLSLPSELRKSLTWDRGMELAR
CCCCCCEEEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHCHHHHHHHCCHHHHHHHHH
HLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIRQYFPKKTCLAQYTQHELDLVAAQLNN
HHHHEEECCCEEEEECCCCHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
RPRKTLKFKTPKEIIERGVALTD
CCCCCEECCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]