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The map label for this gene is ygfA

Identifier: 218696507

GI number: 218696507

Start: 3281355

End: 3281903

Strand: Direct

Name: ygfA

Synonym: EC55989_3199

Alternate gene names: 218696507

Gene position: 3281355-3281903 (Clockwise)

Preceding gene: 218696506

Following gene: 218696510

Centisome position: 63.66

GC content: 52.09

Gene sequence:

>549_bases
ATGATTCGGCAACGTCGTCGTGCGTTAACGCCGGAACAACAGCAGGAAATGGGTCAACAAGCCGCTACCCGGATGATGAC
TTATCCCCCGGTGGTGATGGCGCATACGGTCGCTGTATTCCTCTCTTTTGATGGTGAACTCGACACCCAGCCGCTAATAG
AACAACTCTGGCGCGCCGGTAAGCGCGTATATCTTCCAGTTTTGCATCCCTTTAGTGCCGGTAATTTGCTGTTCCTGAAT
TACCATCCGCAAAGCGAACTGGTGATGAACAGGTTGAAGATCCATGAGCCAAAACTGGATGTGCGTGACGTGCTACCCCT
TTCCCGATTAGACGTGCTGATCACACCGCTGGTCGCCTTTGATGAGTACGGTCAGCGCCTGGGAATGGGCGGTGGTTTTT
ATGATCGGACCTTACAAAACTGGCAGCACTATAAAACGCAACCGGTGGGTTATGCGCATGATTGTCAGTTGGTGGAAAAA
CTCCCCGTTGAAGAGTGGGATATCCCTCTTCCTGCGGTGGTTACACCGTCGAAAGTCTGGGAGTGGTAA

Upstream 100 bases:

>100_bases
GCCTGCGGCGGCATCTCGGAGATTCCCTTCTTATCTGGCACCAGCCATGACGCAACTACCAGAACTCCCACTGATATTAT
CCCGACAAGAAATCCGCAAA

Downstream 100 bases:

>100_bases
GGGCGATACACCTGCATCGCCCTGATTGACATCGTTGATTCTTTAACCTAATTTAGTGAGTAAGGGTAAGGGAGGATTGC
TCCTCCCCTGTGACTGACTG

Product: putative ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MIRQRRRALTPEQQQEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLN
YHPQSELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQHYKTQPVGYAHDCQLVEK
LPVEEWDIPLPAVVTPSKVWEW

Sequences:

>Translated_182_residues
MIRQRRRALTPEQQQEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLN
YHPQSELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQHYKTQPVGYAHDCQLVEK
LPVEEWDIPLPAVVTPSKVWEW
>Mature_182_residues
MIRQRRRALTPEQQQEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLN
YHPQSELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQHYKTQPVGYAHDCQLVEK
LPVEEWDIPLPAVVTPSKVWEW

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family

Homologues:

Organism=Homo sapiens, GI5453746, Length=181, Percent_Identity=25.414364640884, Blast_Score=71, Evalue=5e-13,
Organism=Escherichia coli, GI1789278, Length=182, Percent_Identity=100, Blast_Score=376, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YGFA_ECO57 (P0AC29)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   F91101
- RefSeq:   NP_289479.1
- RefSeq:   NP_311809.3
- ProteinModelPortal:   P0AC29
- SMR:   P0AC29
- MINT:   MINT-1249433
- EnsemblBacteria:   EBESCT00000026759
- EnsemblBacteria:   EBESCT00000057254
- GeneID:   916397
- GeneID:   958390
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4249
- KEGG:   ecs:ECs3782
- GeneTree:   EBGT00050000010573
- HOGENOM:   HBG325345
- OMA:   YAHDCQQ
- ProtClustDB:   PRK10333
- BioCyc:   ECOL83334:ECS3782-MONOMER
- InterPro:   IPR002698
- Gene3D:   G3DSA:3.40.50.10420
- PANTHER:   PTHR23407:SF1
- PIRSF:   PIRSF006806
- TIGRFAMs:   TIGR02727

Pfam domain/function: PF01812 5-FTHF_cyc-lig

EC number: NA

Molecular weight: Translated: 21106; Mature: 21106

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: BINDING 167-167

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRQRRRALTPEQQQEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAG
CCCCHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHCC
KRVYLPVLHPFSAGNLLFLNYHPQSELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAF
CEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHH
DEYGQRLGMGGGFYDRTLQNWQHYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVW
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCEECCHHHC
EW
CC
>Mature Secondary Structure
MIRQRRRALTPEQQQEMGQQAATRMMTYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAG
CCCCHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHCC
KRVYLPVLHPFSAGNLLFLNYHPQSELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAF
CEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHHHHHH
DEYGQRLGMGGGFYDRTLQNWQHYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVW
HHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCEECCHHHC
EW
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796