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The map label for this gene is argA

Identifier: 218696414

GI number: 218696414

Start: 3166392

End: 3167723

Strand: Direct

Name: argA

Synonym: EC55989_3094

Alternate gene names: 218696414

Gene position: 3166392-3167723 (Clockwise)

Preceding gene: 218696410

Following gene: 218696427

Centisome position: 61.43

GC content: 52.63

Gene sequence:

>1332_bases
GTGGTAAAGGAACGTAAAACCGAGTTGGTCGAGGGATTCCGCCATTCGGTTCCCTATATCAATACCCACCGGGGAAAAAC
GTTTGTCATCATGCTCGGCGGTGAAGCCATTGAGCATGAGAATTTCTCCAGTATCGTTAATGATATCGGGTTATTGCACA
GCCTCGGCATCCGTCTGGTGGTGGTCTATGGCGCACGTCCGCAGATCGACGCAAATCTGGCTGCGCATCACCACGAACCG
CTGTATCACAAGAATATACGTGTGACCGACGCCAAAACACTGGAACTGGTGAAGCAGGCTGCGGGAACATTGCAACTGGA
CATCACTGCTCGCCTGTCGATGAGTCTCAATAACACGCCGCTACAGGGCGCGCATATCAACGTCGTCAGTGGCAATTTTA
TTATTGCCCAGCCGCTGGGCGTCGATGACGGCGTGGATTACTGCCATAGCGGGCGTATCCGGCGGATTGATGAAGACGCG
ATCCATCGTCAACTGGACAGCGGTGCAATAGTGCTAATGGGGCCGGTCGCTGTTTCAGTCACTGGCGAGAGCTTTAACCT
GACCTCGGAAGAGATTGCCACTCAACTGGCCATCAAACTGAAAGCTGAGAAGATGATTGGTTTTTGCTCTTCCCAGGGCG
TCACTAATGACGACGGTGATATTGTCTCCGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAA
GGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAGCCTGCCGCAGCGGCGTGCGTCGCTGTCATTT
AATCAGTTATCAGGAAGATGGCGCGCTGTTGCAAGAGTTGTTCTCACGCGACGGTATCGGTACGCAGATTGTGATGGAAA
GCGCCGAGCAGATTCGTCGCGCAACAATCAACGATATTGGCGGTATTCTGGAGTTGATTCGCCCACTGGAGCAGCAAGGT
ATTCTGGTACGCCGTTCTCGCGAGCAGCTGGAGATGGAAATCGACAAATTCACCATTATTCAGCGCGATAACACGACTAT
TGCCTGCGCCGCGCTCTATCCGTTCCCGGAAGAGAAGATTGGGGAAATGGCCTGTGTGGCAGTTCACCCGGATTACCGCA
GTTCATCAAGGGGTGAAGTTCTGCTGGAACGCATTGCCGCTCAGGCGAAGCAGAGCGGCTTAAGCAAATTGTTTGTGCTG
ACCACGCGCAGTATTCACTGGTTCCAGGAACGTGGATTTACCCCAGTGGATATTGATTTACTGCCCGAGAGCAAAAAGCA
GTTGTACAACTACCAGCGTAAATCCAAAGTGTTGATGGCGGATTTAGGGTAA

Upstream 100 bases:

>100_bases
TGATAAAAAAGTCGCTCTCGCATAAAATTTACACTTGCACCCTGCGAAAAAACAGAATAAAAATACACTAATTTCGAATA
ATCATGCAAAGAGGTGTGCC

Downstream 100 bases:

>100_bases
GTGATGAAAATCGTCGGATGCGACATGCGTAACACTCGTACGTCGCATCCGGCAATTACGTTTATTCCCGTGAACTAAAC
AACGCCGCCAGACCACTGCG

Product: N-acetylglutamate synthase

Products: NA

Alternate protein names: N-acetylglutamate synthase; AGS; NAGS

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEP
LYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQEEK
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQG
ILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLFVL
TTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG

Sequences:

>Translated_443_residues
MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEP
LYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQEEK
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQG
ILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLFVL
TTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG
>Mature_443_residues
MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEP
LYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQEEK
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQG
ILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLFVL
TTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG

Specific function: Arginine biosynthesis; first step. [C]

COG id: COG0548

COG function: function code E; Acetylglutamate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain

Homologues:

Organism=Escherichia coli, GI1789181, Length=443, Percent_Identity=100, Blast_Score=911, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320913, Length=290, Percent_Identity=21.3793103448276, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ARGA_ECO57 (P0A6C7)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C91088
- PIR:   E85933
- RefSeq:   NP_289370.1
- RefSeq:   NP_311702.1
- ProteinModelPortal:   P0A6C7
- SMR:   P0A6C7
- PRIDE:   P0A6C7
- EnsemblBacteria:   EBESCT00000027119
- EnsemblBacteria:   EBESCT00000059088
- GeneID:   916513
- GeneID:   957960
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4135
- KEGG:   ecs:ECs3675
- GeneTree:   EBGT00050000010674
- OMA:   APYIHAF
- ProtClustDB:   PRK05279
- BioCyc:   ECOL83334:ECS3675-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01105
- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR001048
- InterPro:   IPR010167
- Gene3D:   G3DSA:3.40.1160.10
- Gene3D:   G3DSA:3.40.630.30
- PIRSF:   PIRSF000423
- TIGRFAMs:   TIGR01890

Pfam domain/function: PF00696 AA_kinase; PF00583 Acetyltransf_1; SSF53633 Aa_kinase; SSF55729 Acyl_CoA_acyltransferase

EC number: =2.3.1.1

Molecular weight: Translated: 49196; Mature: 49196

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS51186 GNAT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLV
CCCHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEE
VVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTP
EEECCCCCCCCCHHHCCCCCCEECCEEEECHHHHHHHHHHCCEEEEEEEEEEEEEECCCC
LQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDAIHRQLDSGAIVLMGPVAVSV
CCCCEEEEEECCEEEEECCCCCCCCHHHCCCCCEECCHHHHHHHCCCCCEEEEECEEEEE
TGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQEEK
ECCCCCCCHHHHHHHHHHHEEHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHC
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRR
CCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
ATINDIGGILELIRPLEQQGILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKI
HHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHEECEEEEEEECCCEEEEEEECCCCHHHH
GEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLFVLTTRSIHWFQERGFTPVDIDL
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHCCCCEEEEEE
LPESKKQLYNYQRKSKVLMADLG
CCCHHHHHHHHHHHCEEEEECCC
>Mature Secondary Structure
MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSSIVNDIGLLHSLGIRLV
CCCHHHHHHHHHHHHCCCEEEECCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEE
VVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAGTLQLDITARLSMSLNNTP
EEECCCCCCCCCHHHCCCCCCEECCEEEECHHHHHHHHHHCCEEEEEEEEEEEEEECCCC
LQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDAIHRQLDSGAIVLMGPVAVSV
CCCCEEEEEECCEEEEECCCCCCCCHHHCCCCCEECCHHHHHHHCCCCCEEEEECEEEEE
TGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDDGDIVSELFPNEAQARVEAQEEK
ECCCCCCCHHHHHHHHHHHEEHHHHHHHHCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHC
GDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGALLQELFSRDGIGTQIVMESAEQIRR
CCCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHH
ATINDIGGILELIRPLEQQGILVRRSREQLEMEIDKFTIIQRDNTTIACAALYPFPEEKI
HHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHEECEEEEEEECCCEEEEEEECCCCHHHH
GEMACVAVHPDYRSSSRGEVLLERIAAQAKQSGLSKLFVLTTRSIHWFQERGFTPVDIDL
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCHHHHHHCCCCEEEEEE
LPESKKQLYNYQRKSKVLMADLG
CCCHHHHHHHHHHHCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796