Click here to switch to the map view.

The map label for this gene is hcaB

Identifier: 218696168

GI number: 218696168

Start: 2894578

End: 2895390

Strand: Direct

Name: hcaB

Synonym: EC55989_2827

Alternate gene names: 218696168

Gene position: 2894578-2895390 (Clockwise)

Preceding gene: 218696167

Following gene: 218696169

Centisome position: 56.15

GC content: 56.21

Gene sequence:

>813_bases
ATGAGCGATCTGCATAACGAGTCCATTTTTATTACCGGCGGCGGATCGGGATTAGGGCTGGCGCTGGTCGAGCGATTTAT
CGAAGAAGGCGCGCAGGTTGCCACGCTGGAACTGTCGGCGGCAAAAGTCGCCAGTCTGCGTCAGCGATTTGGCGAACATA
TTCTGGCGGTGGAAGGTAACGTGACCTGTTATGCCGATTATCAACGCGCGGTCGATCAGATCCTGACTCGTTCCGGCAAG
CTGGATTGTTTTATCGGCAATGCAGGCATCTGGGATCACAATGCCTCACTGGTTAATACTCCCGCAGAGACGCTCGAAAC
CGGCTTCCACGAGCTGTTTAACGTCAATGTTCTCGGTTACCTGCTGGGCGCAAAAGCCTGCGCTCCGGCGTTAATCGCCA
GTGAAGGCAGCATGATTTTCACACTGTCAAATGCCGCCTGGTATCCTGGCGGCGGTGGCCCGCTGTACACCGCCAGTAAA
CATGCCGCAACCGGACTTATTCGCCAACTGGCTTATGAACTGGCACCGAAAGTGCGGGTGAATGGCGTCGGCCCGTGTGG
TATGGCCAGCGACCTGCGCGGCCCACAGGCGCTCGGGCAAAGTGAAACCTCGATAATGCAGTCTCTGACGCCGGAGAAAA
TTGCCGCCATTTTACCGCTGCAATTTTTCCCGCAACCGGCGGATTTTACGGGGCCGTATGTGATGTTGGCATCGCGGCGC
AATAATCGCGCATTAAGCGGTGTGATGATCAACGCTGATGCGGGTTTAGCGATTCGCGGCATTCGCCACGTAGCGGCTGG
GCTGGATCTTTAA

Upstream 100 bases:

>100_bases
CGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATC
GACTTACCGGAGGCGCAGCC

Downstream 100 bases:

>100_bases
GGAAGCACGATGAAAGAAAAAACGATCATTATTGTCGGTGGCGGGCAAGCGGCGGCAATGGCTGCGGCCTCGCTACGCCA
GCAAGGGTTCACCGGTGAGC

Product: 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase

Products: NA

Alternate protein names: 2,3-dihydroxy-2,3-dihydrophenylpropionate dehydrogenase; CI-dihydrodiol dehydrogenase; Cis-3-(2-carboxyethenyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase; Cis-3-(2-carboxyethyl)-3,5-cyclohexadiene-1,2-diol dehydrogenase; PP-dihydrodiol dehydrogenase

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVASLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGK
LDCFIGNAGIWDHNASLVNTPAETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAILPLQFFPQPADFTGPYVMLASRR
NNRALSGVMINADAGLAIRGIRHVAAGLDL

Sequences:

>Translated_270_residues
MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVASLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGK
LDCFIGNAGIWDHNASLVNTPAETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAILPLQFFPQPADFTGPYVMLASRR
NNRALSGVMINADAGLAIRGIRHVAAGLDL
>Mature_269_residues
SDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVASLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKL
DCFIGNAGIWDHNASLVNTPAETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASKH
AATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAILPLQFFPQPADFTGPYVMLASRRN
NRALSGVMINADAGLAIRGIRHVAAGLDL

Specific function: Converts 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol) into 3-(2,3- dihydroxylphenyl)propanoic acid (DHPP) and 2,3-dihydroxicinnamic acid (DHCI), respectively

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family

Homologues:

Organism=Homo sapiens, GI5031737, Length=194, Percent_Identity=30.4123711340206, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI33667109, Length=194, Percent_Identity=30.4123711340206, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI32483357, Length=241, Percent_Identity=26.1410788381743, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI4758504, Length=234, Percent_Identity=29.4871794871795, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI40254992, Length=230, Percent_Identity=27.3913043478261, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI59889578, Length=226, Percent_Identity=28.3185840707965, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1788891, Length=270, Percent_Identity=99.6296296296296, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI2367365, Length=241, Percent_Identity=27.8008298755187, Blast_Score=61, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI72000259, Length=270, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17563726, Length=271, Percent_Identity=26.9372693726937, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17560332, Length=277, Percent_Identity=23.4657039711191, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17560150, Length=265, Percent_Identity=25.6603773584906, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17561402, Length=263, Percent_Identity=28.1368821292776, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17559104, Length=267, Percent_Identity=23.5955056179775, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17508895, Length=188, Percent_Identity=28.1914893617021, Blast_Score=64, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17562990, Length=264, Percent_Identity=25.7575757575758, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6322227, Length=203, Percent_Identity=29.064039408867, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI23397609, Length=184, Percent_Identity=31.5217391304348, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24643142, Length=187, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17737361, Length=270, Percent_Identity=27.4074074074074, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HCAB_ECO24 (A7ZPY4)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001463863.1
- ProteinModelPortal:   A7ZPY4
- SMR:   A7ZPY4
- STRING:   A7ZPY4
- EnsemblBacteria:   EBESCT00000022533
- GeneID:   5587581
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_2826
- eggNOG:   COG1028
- GeneTree:   EBGT00070000031731
- HOGENOM:   HBG750976
- OMA:   PNAGIWD
- ProtClustDB:   PRK06200
- BioCyc:   ECOL331111:ECE24377A_2826-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01647
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR19410
- PRINTS:   PR00081
- PRINTS:   PR00080

Pfam domain/function: PF00106 adh_short

EC number: 1.3.1.-

Molecular weight: Translated: 28470; Mature: 28339

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00061 ADH_SHORT

Important sites: ACT_SITE 156-156 BINDING 143-143

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVASLRQRFGEHILAVEGN
CCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCEEEEEECC
VTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPAETLETGFHELFNVNVLGY
EEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHH
LLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASKHAATGLIRQLAYELAPKVRV
HHHHHHHCCHHEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEE
NGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAILPLQFFPQPADFTGPYVMLASRR
CCCCCCCCHHHCCCCHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
NNRALSGVMINADAGLAIRGIRHVAAGLDL
CCCEEEEEEEECCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVASLRQRFGEHILAVEGN
CCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCEEEEEECC
VTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPAETLETGFHELFNVNVLGY
EEEEEHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHH
LLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASKHAATGLIRQLAYELAPKVRV
HHHHHHHCCHHEECCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEE
NGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAILPLQFFPQPADFTGPYVMLASRR
CCCCCCCCHHHCCCCHHCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
NNRALSGVMINADAGLAIRGIRHVAAGLDL
CCCEEEEEEEECCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA