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The map label for this gene is mnmC

Identifier: 218695923

GI number: 218695923

Start: 2639403

End: 2641409

Strand: Direct

Name: mnmC

Synonym: EC55989_2568

Alternate gene names: 218695923

Gene position: 2639403-2641409 (Clockwise)

Preceding gene: 218695920

Following gene: 218695930

Centisome position: 51.2

GC content: 52.72

Gene sequence:

>2007_bases
GTGAAACACTACTCCATACAACCTGCCAACCTCGAATTTAATGCTGAGGGTACACCTGTTTCCCGAGATTTTGACGATGT
CTATTTTTCCAACGATAACGGGCTGGAAGAGACGCGTTATGTTTTTCTGGGAGGCAACCAATTAGAGGTACGCTTTCCTG
AGCATCCACATCCTCTGTTTGTGGTAGCAGAGAGCGGCTTCGGCACCGGATTAAACTTCCTGACGCTATGGCAGGCATTT
GATCAGTTTCGCGAAGCGCATCCGCAAGCGCAATTACAACGCTTACATTTCATTAGTTTTGAGAAATTTCCCCTCACCCG
TGCGGATTTAGCCTTAGCGCATCAACACTGGCCGGAACTGGCTCCGTGGGCAGAACAACTTCAGGCGCAGTGGCCAATGC
CCTTGCCCGGTTGCCATCGTTTATTGCTCGATGAAGGCCGCGTGACGCTGGATTTATGGTTTGGCGATATTAACGAACTG
ACCAGCCAACTGGACGATTCGCTAAATCAAAAAGTAGATGCCTGGTTTCTGGACGGCTTTGCGCCAGCGAAAAACCCGGA
TATGTGGACGCAAAATCTGTTTAACGCCATGGCAAAGTTGGCGCGTCCGGGCGGCACGCTGGCGACATTTACGTCTGCCG
GTTTTGTCCGCCGCGGTTTGCAGGACGCCGGATTCACGATGCAAAAACGTAAGGGCTTTGGGCGCAAACGGGAAATGCTT
TGCGGGGTGATGGAACAGACATTACCGCTCCCCTGCTCCGCGCCGTGGTTTAACCGCACGGGCAGCAGCAAACGGGAAGC
GGCGATTATCGGCGGTGGTATTGCCAGCGCGTTGTTGTCGCTGGCGCTATTACGGCGCGGCTGGCAGGTAACGCTTTATT
GCGCGGATGAGGCCCCCGCACTGGGTGCTTCCGGCAATCGCCAGGGGGCGCTGTATCCGTTATTAAGCAAACACGATGAG
GCGCTAAACCGCTTTTTCTCTAATGCGTTTACTTTTGCTCGTCGGTTTTACGACCAATTACCCGTTAAATTTGATCATGA
CTGGTGCGGCGTCACGCAGTTAGGCTGGAATGAGAAAAGCCAGCATAAAATCGCACAGATGTTGTCAATGGATTTACCCG
CAGAACTGGCTGTAGCCGTTGAGGCAAATGCGGTTGAACAAATTACGGGCGTTGCGACAAATTGCAGCGGCATTACTTAT
CCGCAAGGTGGTTGGCTGTGCCCAGCAGAACTGACCCGTAATGTGCTGGAACTGGCGCAACAGCAGGGTTTGCAGATTTA
TTATCAATATCAGTTACAGAATTTATCCCGTAAGGATGACTGTTGGTTGTTGAATTTTGCAGGAGATCAGCAAGCAACAC
ACAGCGTAGTGGTACTGGCGAACGGGCATCAAATCAGCCGATTCAGCCATACGTCGACTCTCCCGGTGTATTCGGTTGCC
GGGCAGGTCAGCCATATTCCGACAACGCCGGAATTGGCAGAGCTGAAGCAGGTGCTGTGCTATGACGGTTATCTCACGCC
ACAAAATCCGGCGAATCAACATCATTGTATTGGTGCCAGTTATCATCGCGGCAGCGAAGATACGGCGTACAGTGAGGACG
ATCAGCAGCAGAATCGCCAGCGGTTGATTGATTGTTTCCCGCAGGCACAGTGGGCAAAAGAGGTTGATGTCAGTGATAAA
GAGGCGCGCTGCGGTGTGCGTTGTGCCACCCGCGATCATCTGCCAATGGTAGGCAATGTTCCCGATTATGAGGCAACACT
CGTGGAATATGCGTCGTTGGCGGAGCAGAAAGATGAGGCGGTAAGCGCGCCGGTTTTTGACGATATCTTTATGTTTGCGG
CTTTAGGTTCTCGCGGTTTGTGTTCTGCCCCGCTGTGTGCCGAGATTCTGGCGGCGCAGATGAGCGACGAACCGATTCCG
ATGGATGCCAGTACGCTGGCGGCGTTAAACCCGAATCGGTTATGGGTGCGGAAATTGTTGAAGGGTAAAGCGGTTAAGGC
GGGGTAA

Upstream 100 bases:

>100_bases
GTACGCCGAACAAGTCCGATCAGCCATTTAATAGAGAAATTTGCGCAGCCTTACACACATCGCTAAGATCGAGCCACCGC
CTGTAAGACGAGTAACTTAC

Downstream 100 bases:

>100_bases
TCTGCTCTCGCAGTGTTTGCCGGATGCGGCGTGAACGCCTTATCCGACCTACGTGTGACGTGTAGGCCTGATAAGACGCG
ATAAGCGTCGCATCAGGCAT

Product: 5-methylaminomethyl-2-thiouridine methyltransferase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAF
DQFREAHPQAQLQRLHFISFEKFPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMAKLARPGGTLATFTSAGFVRRGLQDAGFTMQKRKGFGRKREML
CGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDE
ALNRFFSNAFTFARRFYDQLPVKFDHDWCGVTQLGWNEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVATNCSGITY
PQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQQATHSVVVLANGHQISRFSHTSTLPVYSVA
GQVSHIPTTPELAELKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDK
EARCGVRCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDIFMFAALGSRGLCSAPLCAEILAAQMSDEPIP
MDASTLAALNPNRLWVRKLLKGKAVKAG

Sequences:

>Translated_668_residues
MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAF
DQFREAHPQAQLQRLHFISFEKFPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMAKLARPGGTLATFTSAGFVRRGLQDAGFTMQKRKGFGRKREML
CGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDE
ALNRFFSNAFTFARRFYDQLPVKFDHDWCGVTQLGWNEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVATNCSGITY
PQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQQATHSVVVLANGHQISRFSHTSTLPVYSVA
GQVSHIPTTPELAELKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDK
EARCGVRCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDIFMFAALGSRGLCSAPLCAEILAAQMSDEPIP
MDASTLAALNPNRLWVRKLLKGKAVKAG
>Mature_668_residues
MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAF
DQFREAHPQAQLQRLHFISFEKFPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMAKLARPGGTLATFTSAGFVRRGLQDAGFTMQKRKGFGRKREML
CGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDE
ALNRFFSNAFTFARRFYDQLPVKFDHDWCGVTQLGWNEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVATNCSGITY
PQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQQATHSVVVLANGHQISRFSHTSTLPVYSVA
GQVSHIPTTPELAELKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDK
EARCGVRCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDIFMFAALGSRGLCSAPLCAEILAAQMSDEPIP
MDASTLAALNPNRLWVRKLLKGKAVKAG

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

Organism=Escherichia coli, GI87082084, Length=668, Percent_Identity=99.4011976047904, Blast_Score=1384, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 74415; Mature: 74415

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLEVRFPEHPHPLF
CCCCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCEEEEECCCCCCCEE
VVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEKFPLTRADLALAHQHWPEL
EEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCC
APWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINELTSQLDDSLNQKVDAWFLDGF
CHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCC
APAKNPDMWTQNLFNAMAKLARPGGTLATFTSAGFVRRGLQDAGFTMQKRKGFGRKREML
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCHHHCCCCHHHHHCCCHHHHHH
CGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPA
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCC
LGASGNRQGALYPLLSKHDEALNRFFSNAFTFARRFYDQLPVKFDHDWCGVTQLGWNEKS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHH
QHKIAQMLSMDLPAELAVAVEANAVEQITGVATNCSGITYPQGGWLCPAELTRNVLELAQ
HHHHHHHHHCCCCCCEEEEEECHHHHHHHCCEECCCCCCCCCCCEEEHHHHHHHHHHHHH
QQGLQIYYQYQLQNLSRKDDCWLLNFAGDQQATHSVVVLANGHQISRFSHTSTLPVYSVA
HCCCEEEEEEEHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCEEEECCCCCCCCHHHHC
GQVSHIPTTPELAELKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQ
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHH
RLIDCFPQAQWAKEVDVSDKEARCGVRCATRDHLPMVGNVPDYEATLVEYASLAEQKDEA
HHHHHCCCHHHHHHCCCCCCHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VSAPVFDDIFMFAALGSRGLCSAPLCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLL
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHEECCCCHHHHHHHH
KGKAVKAG
CCCCCCCC
>Mature Secondary Structure
MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLGGNQLEVRFPEHPHPLF
CCCCCCCCCCEEECCCCCCCCCCCCCEEECCCCCCCCEEEEEECCCEEEEECCCCCCCEE
VVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEKFPLTRADLALAHQHWPEL
EEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHEECCCCCCHHHHHHHHHCCCCC
APWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINELTSQLDDSLNQKVDAWFLDGF
CHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHEEECCC
APAKNPDMWTQNLFNAMAKLARPGGTLATFTSAGFVRRGLQDAGFTMQKRKGFGRKREML
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCHHHCCCCHHHHHCCCHHHHHH
CGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIASALLSLALLRRGWQVTLYCADEAPA
HHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEECCCCCC
LGASGNRQGALYPLLSKHDEALNRFFSNAFTFARRFYDQLPVKFDHDWCGVTQLGWNEKS
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHH
QHKIAQMLSMDLPAELAVAVEANAVEQITGVATNCSGITYPQGGWLCPAELTRNVLELAQ
HHHHHHHHHCCCCCCEEEEEECHHHHHHHCCEECCCCCCCCCCCEEEHHHHHHHHHHHHH
QQGLQIYYQYQLQNLSRKDDCWLLNFAGDQQATHSVVVLANGHQISRFSHTSTLPVYSVA
HCCCEEEEEEEHHCCCCCCCEEEEEECCCCCCCCEEEEEECCCEEEECCCCCCCCHHHHC
GQVSHIPTTPELAELKQVLCYDGYLTPQNPANQHHCIGASYHRGSEDTAYSEDDQQQNRQ
CCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHH
RLIDCFPQAQWAKEVDVSDKEARCGVRCATRDHLPMVGNVPDYEATLVEYASLAEQKDEA
HHHHHCCCHHHHHHCCCCCCHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
VSAPVFDDIFMFAALGSRGLCSAPLCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLL
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHEECCCCHHHHHHHH
KGKAVKAG
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA