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The map label for this gene is yfbB

Identifier: 218695866

GI number: 218695866

Start: 2578857

End: 2579615

Strand: Reverse

Name: yfbB

Synonym: EC55989_2511

Alternate gene names: 218695866

Gene position: 2579615-2578857 (Counterclockwise)

Preceding gene: 218695867

Following gene: 218695865

Centisome position: 50.04

GC content: 56.52

Gene sequence:

>759_bases
ATGATCCTGCACGCGCAGGCAAAACACGGAAAACCAGGTTTACCCTGGCTGGTGTTTTTGCACGGTTTTTCCGGCGATTG
CCACGAATGGCAAGAAGTGGGCGAGGCGTTTGCCGACTACTCACGGTTGTATGTTGATCTCCCAGGTCATGGTGGTTCGG
CGGCGATTAGCGTCGATGGATTTGATGATGTCACCGACTTACTGCGTAAAACCTTGGTTAGTTACAACATCCTTGACTTC
TGGCTGGTGGGGTACTCGCTTGGCGGACGGGTGGCGATGATGGCGGCTTGCCAGGGGCTGGCGGGGCTTTGTGGGGTTAT
TGTCGAAGGCGGGCATCCGGGGCTGCAAAATGCTGAACAACGTGCGGAACGTCAGCGTTCCGATCGCCAATGGGCGCAGC
GTTTTCGCACAGAACCGTTAACGGCGGTATTTGCCGACTGGTATCAACAGCCTGTTTTTGCCTCGCTCAATGACGATCAA
CGCCGGGAGCTGGTGGCGCTGCGCAGCAACAATAATGGCGCAACCCTTGCCGCCATGCTGGAGGCGACTTCTCTCGCCGT
CCAGCCTGATTTACGTGCTAACCTTAGCGCCCGCACATTTGCGTTTTATTATTTATGTGGTGAACGTGACAGCAAATTCC
GCGCCCTGGCGGCGGAACTGGCTGCCGACTGCCATGTCATTCCTCGCGCCGGACATAACGCGCATCGGGAAAATCCCGCT
GGCGTTATCGCAAGTCTGGCGCAGATCTTGCGTTTCTGA

Upstream 100 bases:

>100_bases
ACGCCTGGCGCACGCCAACCACCACGGTGATTGAAATGGTGGTTAACGACACCGATGGTGCGCAAACGCTCCAGCAACTT
CTGGCGCAGGTAAGCCATTT

Downstream 100 bases:

>100_bases
CTAAAGGACACAATATGATTTATCCTGATGAAGCAATGCTTTACGCACCGGTTGAATGGCACGACTGCTCCGAAGGTTTC
GAGGACATTCGTTATGAAAA

Product: acyl-CoA thioester hydrolase YfbB

Products: NA

Alternate protein names: SHCHC synthase

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDF
WLVGYSLGGRVAMMAACQGLAGLCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNAHRENPA
GVIASLAQILRF

Sequences:

>Translated_252_residues
MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDF
WLVGYSLGGRVAMMAACQGLAGLCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNAHRENPA
GVIASLAQILRF
>Mature_252_residues
MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDF
WLVGYSLGGRVAMMAACQGLAGLCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNAHRENPA
GVIASLAQILRF

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family

Homologues:

Organism=Escherichia coli, GI1788598, Length=252, Percent_Identity=99.2063492063492, Blast_Score=515, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MENH_ECO24 (A7ZP82)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001463611.1
- ProteinModelPortal:   A7ZP82
- SMR:   A7ZP82
- STRING:   A7ZP82
- EnsemblBacteria:   EBESCT00000020200
- GeneID:   5586849
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_2559
- eggNOG:   COG0596
- GeneTree:   EBGT00050000011875
- HOGENOM:   HBG748003
- OMA:   ESCHPGL
- ProtClustDB:   PRK11126
- BioCyc:   ECOL331111:ECE24377A_2559-MONOMER
- HAMAP:   MF_01660
- InterPro:   IPR000073
- InterPro:   IPR022485
- TIGRFAMs:   TIGR03695

Pfam domain/function: PF00561 Abhydrolase_1

EC number: =4.2.99.20

Molecular weight: Translated: 27698; Mature: 27698

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLPGHGGSAAISVDG
CEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEECC
FDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAGLCGVIVEGGHPGLQNAEQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
RAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQRRELVALRSNNNGATLAAML
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCHHHHEEEEEECCCCCCHHHHHH
EATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNAHRENPA
HHHHHEECCCHHCCCCHHEEEEEEEECCCCCHHHHHHHHHHHCCEEECCCCCCCCCCCHH
GVIASLAQILRF
HHHHHHHHHHCC
>Mature Secondary Structure
MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLYVDLPGHGGSAAISVDG
CEEECCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCEEEEECC
FDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAGLCGVIVEGGHPGLQNAEQ
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
RAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQRRELVALRSNNNGATLAAML
HHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCHHHHEEEEEECCCCCCHHHHHH
EATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAAELAADCHVIPRAGHNAHRENPA
HHHHHEECCCHHCCCCHHEEEEEEEECCCCCHHHHHHHHHHHCCEEECCCCCCCCCCCHH
GVIASLAQILRF
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA