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The map label for this gene is napB

Identifier: 218695815

GI number: 218695815

Start: 2512295

End: 2512744

Strand: Reverse

Name: napB

Synonym: EC55989_2456

Alternate gene names: 218695815

Gene position: 2512744-2512295 (Counterclockwise)

Preceding gene: 218695816

Following gene: 218695814

Centisome position: 48.75

GC content: 55.56

Gene sequence:

>450_bases
ATGAAAAGCCATGACCTGAAGAAAGCGCTGTGTCAATGGACGGCGATGCTGGCCCTGGTGGTAAGCGGCGCGGTTTGGGC
GGCTAATGGCGTCGATTTTAGCCAGTCGCCGGAAGTATCCGGGACACAGGAAGGGGCCATTCGGATGCCAAAAGAGCAGG
AACGGATGCCGCTGAACTATGTGAATCAGCCGCCAATGATCCCGCATAGCGTTGAAGGTTATCAGGTAACGACCAATACC
AACCGCTGCTTGCAGTGCCACGGTGTCGAAAGTTATCGCACCACTGGCGCGCCGCGTATCAGTCCTACTCACTTTATGGA
CAGCGACGGCAAAGTGGGCGCGGAAGTGGCTCCGCGTCGTTATTTCTGTCTGCAATGTCACGTACCGCAGGCCGATACCG
CGCCAATCGTGGGGAATACCTTTACCCCATCAAAAGGTTACGGGAAATAA

Upstream 100 bases:

>100_bases
AGGTCACCAGCCGCGATTGCATGACGTGCGGTCGCTGCGTGGATGTCTGTTCTGAGGATGTATTTACAATAACTACACGA
TGGAGTTCGGGAGCGAAATC

Downstream 100 bases:

>100_bases
GAGGTTATTATGGGAAATTCTGACCGTAAGCCTGGTCTGATTAAGCGCCTGTGGAAATGGTGGCGTACCCCCAGCCGTCT
GGCGCTGGGGACGCTGCTGT

Product: citrate reductase cytochrome c-type subunit

Products: reduced acceptor; NO3- [C]

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQEGAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNT
NRCLQCHGVESYRTTGAPRISPTHFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK

Sequences:

>Translated_149_residues
MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQEGAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNT
NRCLQCHGVESYRTTGAPRISPTHFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK
>Mature_149_residues
MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQEGAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNT
NRCLQCHGVESYRTTGAPRISPTHFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=149, Percent_Identity=99.3288590604027, Blast_Score=315, Evalue=9e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: NA

Molecular weight: Translated: 16311; Mature: 16311

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQEGAIRMPKEQERMPLNY
CCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCEECCCHHHCCCCCC
VNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISPTHFMDSDGKVGAEVAPRR
CCCCCCCCCCCCCEEEEECCCCEEEECCCCHHHCCCCCCCCCCCEECCCCCCCCCCCCCE
YFCLQCHVPQADTAPIVGNTFTPSKGYGK
EEEEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQEGAIRMPKEQERMPLNY
CCCCHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCEECCCHHHCCCCCC
VNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISPTHFMDSDGKVGAEVAPRR
CCCCCCCCCCCCCEEEEECCCCEEEECCCCHHHCCCCCCCCCCCEECCCCCCCCCCCCCE
YFCLQCHVPQADTAPIVGNTFTPSKGYGK
EEEEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Acceptor; nitrite [C]

Specific reaction: Acceptor + nitrite = reduced acceptor + NO3- [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 8039676 [H]