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The map label for this gene is ccmF

Identifier: 218695808

GI number: 218695808

Start: 2506987

End: 2508930

Strand: Reverse

Name: ccmF

Synonym: EC55989_2449

Alternate gene names: 218695808

Gene position: 2508930-2506987 (Counterclockwise)

Preceding gene: 218695809

Following gene: 218695807

Centisome position: 48.67

GC content: 57.2

Gene sequence:

>1944_bases
ATGATGCCGGAAATTGGTAACGGGCTGCTGTGTCTGGCGCTAGGAATTGCGCTGCTGCTGTCCGTGTATCCGCTATGGGG
CGTAGCGCGCGGAGATGCGCGCATGATGGCGTCTTCCCGCTTGTTTGCCTGGCTGCTGTTTATGTCTGTGGCTGGCGCAT
TTCTGGTACTGGTCAATGCCTTCGTGGTCAACGACTTCACCGTCACCTATGTTGCCAGCAACTCCAATACCCAGCTTCCG
GTGTGGTATCGCGTGGCGGCTACCTGGGGCGCGCATGAAGGCTCGCTCCTGCTGTGGGTGCTGCTGATGAGCGGCTGGAC
CTTTGCGGTAGCGATTTTTAGTCAGCGTATTCCGCTGGATATTGTGGCCCGCGTACTGGCGATAATGGGGATGGTCAGCG
TCGGCTTTTTGCTGTTCATTCTCTTTACCTCTAACCCGTTCTCACGCACGTTGCCGAACTTCCCGATTGAAGGGCGCGAT
CTTAACCCGCTGTTACAGGATCCGGGGCTGATCTTCCATCCGCCTCTGCTCTATATGGGGTACGTGGGGTTCTCGGTGGC
GTTTGCTTTTGCCATTGCTTCTTTGTTGAGCGGGCGTCTGGACAGCACTTATGCGCGTTTTACTCGTCCGTGGACGCTGG
CGGCGTGGATCTTCTTGACGCTCGGCATTGTGCTCGGTTCCGCATGGGCCTATTACGAACTCGGCTGGGGCGGCTGGTGG
TTCTGGGATCCGGTAGAAAACGCCTCGTTTATGCCGTGGCTGGTGGGGACTGCGCTGATGCACTCACTGGCGGTCACTGA
ACAACGCGCCAGCTTCAAAGCGTGGACATTACTGCTGGCAATCAGTGCCTTCTCGTTGTGTCTGCTGGGGACTTTCCTGG
TGCGTTCCGGCGTGCTGGTATCGGTACACGCGTTTGCCTCTGATCCGGCACGCGGTATGTTTATCCTCGCCTTTATGGTA
CTGGTGATTGGTGGTTCGCTGCTGCTGTTTGCCGCGCGTGGACACAAAGTTCGCTCACGCGTAAACAATGCGCTGTGGTC
GCGGGAATCTCTGCTGTTAGCGAACAACGTTTTGCTGGTCGCCGCGATGCTGGTGGTATTACTGGGGACGCTGCTGCCGC
TGGTGCACAAGCAACTGGGACTGGGCAGTATTTCGATTGGCGAACCGTTCTTCAACACCATGTTTACCTGGCTGATGGTG
CCGTTTGCGCTGCTGCTTGGTGTCGGTCCTCTGGTGCGCTGGGGGCGCGATCGCCCACGTAAAATCCGCAATTTATTGAT
TATCGCCTTCATCTCAACGCTGGTGCTGTCGCTGCTGCTGCCGTGGCTGTTCGAAAGCAAAGTTGTGGCGATGACGGTGC
TCGGCCTGGCAATGGCCTGCTGGATTGCTGTGCTGGCAATTGCGGAAGCTGCGCTGCGTATTTCACGTGGCACGAAAACC
ACCTTCAGTTATTGGGGAATGGTGGCGGCTCACCTGGGGCTGGCAGTGACAATTGTTGGTATTGCCTTTAGCCAGAACTA
TAGCGTTGAGCGTGATGTGCGCATGAAGTCCGGCGATAGCGTCGATATTCATGAATATCGCTTCACCTTCCGTGATGTCA
AAGAGGTGACTGGCCCGAACTGGCGTGGCGGTGTGGCGACTATCGGCGTAACGCGCGATGGCAAGCCGGAAACGGTGCTG
TATGCGGAAAAACGTTATTACAACACTGCCGGGTCGATGATGACCGAAGCGGCAATTGACGGCGGCATCACGCGTGACCT
GTACGCCGCGCTCGGTGAAGAGCTGGAAAACGGCGCGTGGGCCGTGCGTCTTTACTACAAACCATTTGTTCGCTGGATTT
GGGCGGGCGGGCTGATGATGGCGTTGGGCGGACTGCTGTGTCTGTTTGATCCTCGCTATCGTAAGCGCGTGAGTCCGCAA
AAAACTGCGCCGGAGGCCGTATGA

Upstream 100 bases:

>100_bases
TGCTGGCGAAACACGACGAAAACTACACGCCGCCAGAAGTTGAGAAAGCGATGGAAGCCAATCACCGTCGCCCGGCGAGT
GTTTATAAGGACCCAGCATC

Downstream 100 bases:

>100_bases
AGCGCAAAGTATTGTTAATTCCGTTGATTATCTTCCTGGCGATTGCCGCGGCGCTGCTGTGGCAGCTGGCGCGTAATGCC
GAAGGGGATGATCCGACCAA

Product: heme lyase, CcmF subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAWLLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLP
VWYRVAATWGAHEGSLLLWVLLMSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIFLTLGIVLGSAWAYYELGWGGWW
FWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWTLLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMV
LVIGGSLLLFAARGHKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFNTMFTWLMV
PFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVAMTVLGLAMACWIAVLAIAEAALRISRGTKT
TFSYWGMVAAHLGLAVTIVGIAFSQNYSVERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVL
YAEKRYYNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGGLLCLFDPRYRKRVSPQ
KTAPEAV

Sequences:

>Translated_647_residues
MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAWLLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLP
VWYRVAATWGAHEGSLLLWVLLMSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIFLTLGIVLGSAWAYYELGWGGWW
FWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWTLLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMV
LVIGGSLLLFAARGHKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFNTMFTWLMV
PFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVAMTVLGLAMACWIAVLAIAEAALRISRGTKT
TFSYWGMVAAHLGLAVTIVGIAFSQNYSVERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVL
YAEKRYYNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGGLLCLFDPRYRKRVSPQ
KTAPEAV
>Mature_647_residues
MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAWLLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLP
VWYRVAATWGAHEGSLLLWVLLMSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIFLTLGIVLGSAWAYYELGWGGWW
FWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWTLLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMV
LVIGGSLLLFAARGHKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFNTMFTWLMV
PFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVAMTVLGLAMACWIAVLAIAEAALRISRGTKT
TFSYWGMVAAHLGLAVTIVGIAFSQNYSVERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVL
YAEKRYYNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGGLLCLFDPRYRKRVSPQ
KTAPEAV

Specific function: Required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase

COG id: COG1138

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family

Homologues:

Organism=Escherichia coli, GI1788524, Length=647, Percent_Identity=100, Blast_Score=1285, Evalue=0.0,
Organism=Escherichia coli, GI1790511, Length=581, Percent_Identity=38.5542168674699, Blast_Score=313, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCMF_ECOLI (P33927)

Other databases:

- EMBL:   U00008
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B64989
- RefSeq:   AP_002792.1
- RefSeq:   NP_416700.1
- ProteinModelPortal:   P33927
- DIP:   DIP-9256N
- IntAct:   P33927
- STRING:   P33927
- EnsemblBacteria:   EBESCT00000000653
- EnsemblBacteria:   EBESCT00000000654
- EnsemblBacteria:   EBESCT00000000655
- EnsemblBacteria:   EBESCT00000015414
- GeneID:   948783
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2184
- KEGG:   eco:b2196
- EchoBASE:   EB1985
- EcoGene:   EG12054
- eggNOG:   COG1138
- GeneTree:   EBGT00050000008901
- HOGENOM:   HBG663906
- OMA:   AEKRFYT
- ProtClustDB:   CLSK880328
- BioCyc:   EcoCyc:EG12054-MONOMER
- Genevestigator:   P33927
- InterPro:   IPR002541
- InterPro:   IPR003567
- InterPro:   IPR003568
- PRINTS:   PR01410
- PRINTS:   PR01411
- TIGRFAMs:   TIGR00353

Pfam domain/function: PF01578 Cytochrom_C_asm

EC number: NA

Molecular weight: Translated: 71390; Mature: 71390

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x15cb49ec)-; HASH(0x1aeaa9b8)-; HASH(0x1b1420d4)-; HASH(0x1b1c0c98)-; HASH(0x197064c4)-; HASH(0x1ad56244)-; HASH(0x1b2648f0)-; HASH(0x1b1fec08)-; HASH(0x1b259380)-; HASH(0x1ae6c640)-; HASH(0x1adec5c4)-; HASH(0x1af59c5c)-; HASH(0x1affe3fc)-; HASH(0x1b00da5c)-; HASH(0x1b22e6a8)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAWLLFMSVAGAFLVLVNA
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLLMSGWTFAVAIFSQRIPLD
HHHCCEEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCHH
IVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRDLNPLLQDPGLIFHPPLLYMG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHCCCCEECCHHHHHH
YVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIFLTLGIVLGSAWAYYELGWGGWW
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHEEEECCCCEE
FWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWTLLLAISAFSLCLLGTFLVRSGVLV
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
SVHAFASDPARGMFILAFMVLVIGGSLLLFAARGHKVRSRVNNALWSRESLLLANNVLLV
EEEECCCCCCCHHHHHHHHHHHHCCHHEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHH
AAMLVVLLGTLLPLVHKQLGLGSISIGEPFFNTMFTWLMVPFALLLGVGPLVRWGRDRPR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHH
KIRNLLIIAFISTLVLSLLLPWLFESKVVAMTVLGLAMACWIAVLAIAEAALRISRGTKT
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
TFSYWGMVAAHLGLAVTIVGIAFSQNYSVERDVRMKSGDSVDIHEYRFTFRDVKEVTGPN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCC
WRGGVATIGVTRDGKPETVLYAEKRYYNTAGSMMTEAAIDGGITRDLYAALGEELENGAW
CCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCE
AVRLYYKPFVRWIWAGGLMMALGGLLCLFDPRYRKRVSPQKTAPEAV
EEEEHHHHHHHHHHHCCHHHHHCCHHHHCCCHHHHCCCCCCCCCCCH
>Mature Secondary Structure
MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAWLLFMSVAGAFLVLVNA
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLLMSGWTFAVAIFSQRIPLD
HHHCCEEEEEEECCCCCCCEEEEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCHH
IVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRDLNPLLQDPGLIFHPPLLYMG
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHCCCCEECCHHHHHH
YVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIFLTLGIVLGSAWAYYELGWGGWW
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHEEEECCCCEE
FWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWTLLLAISAFSLCLLGTFLVRSGVLV
EECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
SVHAFASDPARGMFILAFMVLVIGGSLLLFAARGHKVRSRVNNALWSRESLLLANNVLLV
EEEECCCCCCCHHHHHHHHHHHHCCHHEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHH
AAMLVVLLGTLLPLVHKQLGLGSISIGEPFFNTMFTWLMVPFALLLGVGPLVRWGRDRPR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCHH
KIRNLLIIAFISTLVLSLLLPWLFESKVVAMTVLGLAMACWIAVLAIAEAALRISRGTKT
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
TFSYWGMVAAHLGLAVTIVGIAFSQNYSVERDVRMKSGDSVDIHEYRFTFRDVKEVTGPN
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHCCCC
WRGGVATIGVTRDGKPETVLYAEKRYYNTAGSMMTEAAIDGGITRDLYAALGEELENGAW
CCCCEEEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCE
AVRLYYKPFVRWIWAGGLMMALGGLLCLFDPRYRKRVSPQKTAPEAV
EEEEHHHHHHHHHHHCCHHHHHCCHHHHCCCHHHHCCCCCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 7635817