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The map label for this gene is yeiL

Identifier: 218695777

GI number: 218695777

Start: 2470757

End: 2471416

Strand: Direct

Name: yeiL

Synonym: EC55989_2416

Alternate gene names: 218695777

Gene position: 2470757-2471416 (Clockwise)

Preceding gene: 218695774

Following gene: 218695784

Centisome position: 47.93

GC content: 45.15

Gene sequence:

>660_bases
ATGAGTGAATCCGCGTTTAAGGATTGCTTTTTAACGGATGTTTCAGCCGATACGCGGCTGTTTCATTTTTTAGCGCGTGA
CTACATTGTGCAGGAAGGGCAACAGCCGTCCTGGCTGTTTTACCTGACGCGAGGCCGCGCCAGGCTTTACGCCACGCTAG
CTAATGGTCGCGTGTCGCTGATCGATTTCTTTGCCGCCCCCTGTTTTATTGGCGAGATTGAGTTAATCGACAAAGACCAT
GAACCGCGTGCGGTGCAGGCTATTGAAGAGTGTTGGTGCCTTGCGCTCCCTATGAAACATTACCGCCCGCGGTTATTAAA
CGACACGCTATTTTTACGAAAACTCTGCGTCACCTTAAGTCATAAAAATTATCGTAATATTGTTTCTTTAACTCAGAATC
AATCATTTCCGTTAGTGAATCGCCTGGCAGCATTTATATTACTCTCGCAGGAAGGTGATCTTTATCACGAAAAGCATACG
CAAGCGGCAGAGTATTTAGGCGTTTCTTATCGACATCTTTTATATGTTCTCGCGCAATTCATTCACGACGGTTTATTAAT
TAAAAGCAAGAAAGGGTATCTAATTAAAAACAGAAAGCAGTTGTCAGGACTGGCGCTGGAGATGGACCCGGAGAATAAAT
TCTCCGGGATGATGCAGTAA

Upstream 100 bases:

>100_bases
TTGAATCTTACCAACCGCGTACGTATGCTAAATATGAGAAATCTCATAGCGGATAAACATCGTGAAAGAAATCCACAATA
ATGATCTTAAGCAGCAATTG

Downstream 100 bases:

>100_bases
AAATTATTTGCAATAGCGCGATTGCCGGATGCAACACTTAATAAGCGCGTACATCCGGCAATGAAACTCAAGCTAAACCA
ATAAAGAACCCGGCAATGGT

Product: DNA-binding transcriptional activator YeiL

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSESAFKDCFLTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDH
EPRAVQAIEECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFILLSQEGDLYHEKHT
QAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ

Sequences:

>Translated_219_residues
MSESAFKDCFLTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDH
EPRAVQAIEECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFILLSQEGDLYHEKHT
QAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ
>Mature_218_residues
SESAFKDCFLTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHE
PRAVQAIEECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFILLSQEGDLYHEKHTQ
AAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ

Specific function: Transcription regulator involved in mid-term, stationary-phase viability under nitrogen starvation. Might control expression of the salvage pathways or in some other way repress the recycling of nucleobases to nucleic acids and enhance their use as genera

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788487, Length=219, Percent_Identity=99.5433789954338, Blast_Score=454, Evalue=1e-129,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR012318
- InterPro:   IPR014710 [H]

Pfam domain/function: PF00027 cNMP_binding [H]

EC number: NA

Molecular weight: Translated: 25338; Mature: 25206

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESAFKDCFLTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRARLYATLANGRVSL
CCCCHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCCCCHH
IDFFAAPCFIGEIELIDKDHEPRAVQAIEECWCLALPMKHYRPRLLNDTLFLRKLCVTLS
HHHHHCCHHCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHC
HKNYRNIVSLTQNQSFPLVNRLAAFILLSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQF
CCCHHHHHHHHCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
IHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ
HHCCEEEECCCCCEEECCHHHCCEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SESAFKDCFLTDVSADTRLFHFLARDYIVQEGQQPSWLFYLTRGRARLYATLANGRVSL
CCCHHHHHHEECCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEEECCCCCHH
IDFFAAPCFIGEIELIDKDHEPRAVQAIEECWCLALPMKHYRPRLLNDTLFLRKLCVTLS
HHHHHCCHHCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHC
HKNYRNIVSLTQNQSFPLVNRLAAFILLSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQF
CCCHHHHHHHHCCCCCCHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHH
IHDGLLIKSKKGYLIKNRKQLSGLALEMDPENKFSGMMQ
HHCCEEEECCCCCEEECCHHHCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]