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The map label for this gene is lysP

Identifier: 218695770

GI number: 218695770

Start: 2462515

End: 2463984

Strand: Reverse

Name: lysP

Synonym: EC55989_2409

Alternate gene names: 218695770

Gene position: 2463984-2462515 (Counterclockwise)

Preceding gene: 218695771

Following gene: 218695769

Centisome position: 47.8

GC content: 53.47

Gene sequence:

>1470_bases
ATGGTTTCCGAAACTAAAACCACAGAAGCGCCGGGCTTACGCCGTGAATTAAAGGCGCGTCACCTGACGATGATTGCCAT
TGGCGGTTCCATCGGTACAGGTCTTTTTGTTGCCTCTGGCGCAACGATTTCTCAGGCAGGTCCGGGCGGGGCATTGCTCT
CGTATATGCTGATTGGCCTGATGGTTTACTTCCTGATGACCAGTCTCGGTGAACTGGCTGCATATATGCCGGTTTCCGGT
TCGTTTGCCACTTACGGTCAGAACTATGTTGAAGAAGGCTTTGGCTTCGCGCTGGGCTGGAACTACTGGTACAACTGGGC
GGTGACTATCGCCGTTGACCTGGTTGCAGCTCAGCTGGTCATGAGCTGGTGGTTCCCGGATACACCGGGCTGGATCTGGA
GTGCGTTGTTCCTCGGCGTTATCTTCCTGCTGAACTACATCTCAGTTCGTGGCTTTGGTGAAGCGGAATACTGGTTCTCA
CTGATCAAAGTCACGACAGTTATTGTCTTTATCATCGTTGGCGTGCTGATGATTATCGGTATCTTCAAAGGCGCGCAGCC
TGCGGGCTGGAGCAACTGGACAATCGGCGAAGCGCCGTTTGCTGGTGGTTTTGCGGCGATGATCGGCGTAGCTATGATTG
TCGGCTTCTCTTTCCAGGGAACCGAGCTGATCGGTATTGCTGCAGGCGAGTCCGAAGATCCGGCGAAAAACATTCCACGC
GCGGTACGTCAGGTGTTCTGGCGAATCCTGTTGTTCTATGTGTTCGCGATCCTGATTATCAGCCTGATTATTCCGTACAC
CGATCCGAGCCTGCTGCGTAACGATGTTAAAGACATCAGCGTTAGTCCGTTCACCCTGGTGTTCCAGCACGCGGGTCTGC
TCTCTGCGGCGGCGGTGATGAACGCAGTTATTCTGACGGCGGTGCTGTCAGCGGGTAACTCCGGTATGTATGCGTCTACT
CGTATGCTGTACACCCTGGCGTGTGACGGTAAAGCGCCGCGCATTTTCGCTAAACTGTCGCGTGGTGGCGTGCCGCGTAA
TGCGCTGTATGCGACGACGGTGATTGCCGGTCTGTGCTTCCTGACCTCCATGTTTGGCAACCAGACGGTATACCTGTGGC
TGCTGAACACCTCCGGGATGACGGGTTTTATCGCCTGGCTGGGGATTGCCATTAGCCACTATCGCTTCCGTCGCGGTTAC
GTATTGCAGGGACACGACATTAACGATCTGCCGTACCGTTCAGGTTTCTTCCCACTGGGGCCGATCTTCGCATTCATTCT
GTGTCTGATTATCACTTTGGGCCAGAACTACGAAGCGTTCCTGAAAGATACCATTGACTGGGGCGGCGTAGCGGCAACGT
ATATTGGTATCCCGCTGTTCCTGATTATTTGGTTCGGCTACAAGCTGATTAAAGGAACTCACTTCGTACGTTACAGCGAA
ATGAAGTTCCCGCAGAACGATAAGAAATAA

Upstream 100 bases:

>100_bases
GCGATCATGAAGGTGTCTTATAACCGTGTATTTCTGCCGGAAGGATTGCCAATCGTCTGCTACAATCGCGCCTCATTTTT
AAGATGGATAGCATTTTTGT

Downstream 100 bases:

>100_bases
GTTTCCTCCCTTCCTTGCTAAGCCCTCTCAACCGAGAGGGCTTTTTCAATTCCATTTCCCTGACAAATCATGCGGATATA
AAATTTAACATTTGGATTGA

Product: lysine transporter

Products: Proton [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATISQAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSG
SFATYGQNYVEEGFGFALGWNYWYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSFQGTELIGIAAGESEDPAKNIPR
AVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDVKDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYAST
RMLYTLACDGKAPRIFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAISHYRFRRGY
VLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGVAATYIGIPLFLIIWFGYKLIKGTHFVRYSE
MKFPQNDKK

Sequences:

>Translated_489_residues
MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATISQAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSG
SFATYGQNYVEEGFGFALGWNYWYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSFQGTELIGIAAGESEDPAKNIPR
AVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDVKDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYAST
RMLYTLACDGKAPRIFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAISHYRFRRGY
VLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGVAATYIGIPLFLIIWFGYKLIKGTHFVRYSE
MKFPQNDKK
>Mature_489_residues
MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATISQAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSG
SFATYGQNYVEEGFGFALGWNYWYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSFQGTELIGIAAGESEDPAKNIPR
AVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDVKDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYAST
RMLYTLACDGKAPRIFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAISHYRFRRGY
VLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGVAATYIGIPLFLIIWFGYKLIKGTHFVRYSE
MKFPQNDKK

Specific function: Permease that is involved in the transport across the cytoplasmic membrane of lysine

COG id: COG0833

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family

Homologues:

Organism=Homo sapiens, GI110347453, Length=369, Percent_Identity=24.390243902439, Blast_Score=76, Evalue=6e-14,
Organism=Escherichia coli, GI1788480, Length=489, Percent_Identity=100, Blast_Score=981, Evalue=0.0,
Organism=Escherichia coli, GI87081708, Length=438, Percent_Identity=40.1826484018265, Blast_Score=327, Evalue=1e-90,
Organism=Escherichia coli, GI48994972, Length=435, Percent_Identity=39.0804597701149, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1786789, Length=418, Percent_Identity=37.799043062201, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI1786302, Length=392, Percent_Identity=35.969387755102, Blast_Score=255, Evalue=5e-69,
Organism=Escherichia coli, GI1790653, Length=422, Percent_Identity=35.0710900473934, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1789017, Length=392, Percent_Identity=34.1836734693878, Blast_Score=238, Evalue=5e-64,
Organism=Escherichia coli, GI87081915, Length=400, Percent_Identity=34.25, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1786602, Length=406, Percent_Identity=36.6995073891626, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI87081869, Length=395, Percent_Identity=24.3037974683544, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082023, Length=335, Percent_Identity=24.1791044776119, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI87082250, Length=236, Percent_Identity=28.3898305084746, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17532491, Length=389, Percent_Identity=24.9357326478149, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6320772, Length=474, Percent_Identity=36.4978902953587, Blast_Score=322, Evalue=1e-88,
Organism=Saccharomyces cerevisiae, GI6324990, Length=487, Percent_Identity=34.9075975359343, Blast_Score=309, Evalue=7e-85,
Organism=Saccharomyces cerevisiae, GI6324061, Length=495, Percent_Identity=33.7373737373737, Blast_Score=305, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6322892, Length=475, Percent_Identity=36.8421052631579, Blast_Score=292, Evalue=7e-80,
Organism=Saccharomyces cerevisiae, GI6324059, Length=476, Percent_Identity=35.5042016806723, Blast_Score=286, Evalue=6e-78,
Organism=Saccharomyces cerevisiae, GI6324924, Length=483, Percent_Identity=33.3333333333333, Blast_Score=275, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6321629, Length=502, Percent_Identity=30.0796812749004, Blast_Score=264, Evalue=3e-71,
Organism=Saccharomyces cerevisiae, GI6324553, Length=429, Percent_Identity=35.4312354312354, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6319824, Length=472, Percent_Identity=29.2372881355932, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6322967, Length=470, Percent_Identity=31.4893617021277, Blast_Score=232, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6321053, Length=464, Percent_Identity=29.0948275862069, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6320717, Length=482, Percent_Identity=27.5933609958506, Blast_Score=227, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6319543, Length=474, Percent_Identity=29.535864978903, Blast_Score=223, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6319542, Length=472, Percent_Identity=27.3305084745763, Blast_Score=217, Evalue=4e-57,
Organism=Saccharomyces cerevisiae, GI6324981, Length=490, Percent_Identity=31.2244897959184, Blast_Score=215, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6320251, Length=480, Percent_Identity=26.0416666666667, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6319608, Length=493, Percent_Identity=27.789046653144, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6320364, Length=566, Percent_Identity=26.3250883392226, Blast_Score=177, Evalue=3e-45,
Organism=Drosophila melanogaster, GI281366235, Length=396, Percent_Identity=23.7373737373737, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI116007820, Length=396, Percent_Identity=23.7373737373737, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24666159, Length=358, Percent_Identity=24.0223463687151, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI221512776, Length=358, Percent_Identity=24.0223463687151, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LYSP_ECOLI (P25737)

Other databases:

- EMBL:   M89774
- EMBL:   U00007
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   C64984
- RefSeq:   AP_002753.1
- RefSeq:   NP_416661.1
- ProteinModelPortal:   P25737
- STRING:   P25737
- EnsemblBacteria:   EBESCT00000003091
- EnsemblBacteria:   EBESCT00000018024
- GeneID:   946667
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2143
- KEGG:   eco:b2156
- EchoBASE:   EB1313
- EcoGene:   EG11337
- eggNOG:   COG0833
- GeneTree:   EBGT00050000009005
- HOGENOM:   HBG492579
- OMA:   PIFAFTL
- ProtClustDB:   PRK10836
- BioCyc:   EcoCyc:LYSP-MONOMER
- Genevestigator:   P25737
- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 53577; Mature: 53577

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b06d980)-; HASH(0x1b13b1e8)-; HASH(0x19ecf400)-; HASH(0x1ad41ea8)-; HASH(0x1b0919e4)-; HASH(0x1aaa9068)-; HASH(0x1acfa13c)-; HASH(0x194f2240)-; HASH(0x1add87d8)-; HASH(0x1a923734)-; HASH(0x1a959f18)-; HASH(0x1b0896ec)-;

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATISQAGPGGALLSYMLIGL
CCCCCCCCCCCCHHHHHHHHHEEEEEECCCCCCCEEEECCCEECCCCCCHHHHHHHHHHH
MVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNYWYNWAVTIAVDLVAAQLV
HHHHHHHHHHHHHHHCCCCCCHHHHCHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHH
MSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFSLIKVTTVIVFIIVGVLMIIG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSFQGTELIGIAAGESEDPAKNIPR
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHH
AVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDVKDISVSPFTLVFQHAGLLSAAAVM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NAVILTAVLSAGNSGMYASTRMLYTLACDGKAPRIFAKLSRGGVPRNALYATTVIAGLCF
HHHHHHHHHHCCCCCCEEHHEEEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
LTSMFGNQTVYLWLLNTSGMTGFIAWLGIAISHYRFRRGYVLQGHDINDLPYRSGFFPLG
HHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHH
PIFAFILCLIITLGQNYEAFLKDTIDWGGVAATYIGIPLFLIIWFGYKLIKGTHFVRYSE
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
MKFPQNDKK
CCCCCCCCH
>Mature Secondary Structure
MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATISQAGPGGALLSYMLIGL
CCCCCCCCCCCCHHHHHHHHHEEEEEECCCCCCCEEEECCCEECCCCCCHHHHHHHHHHH
MVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNYWYNWAVTIAVDLVAAQLV
HHHHHHHHHHHHHHHCCCCCCHHHHCHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHH
MSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFSLIKVTTVIVFIIVGVLMIIG
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSFQGTELIGIAAGESEDPAKNIPR
HHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHH
AVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDVKDISVSPFTLVFQHAGLLSAAAVM
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NAVILTAVLSAGNSGMYASTRMLYTLACDGKAPRIFAKLSRGGVPRNALYATTVIAGLCF
HHHHHHHHHHCCCCCCEEHHEEEEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHH
LTSMFGNQTVYLWLLNTSGMTGFIAWLGIAISHYRFRRGYVLQGHDINDLPYRSGFFPLG
HHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCHH
PIFAFILCLIITLGQNYEAFLKDTIDWGGVAATYIGIPLFLIIWFGYKLIKGTHFVRYSE
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
MKFPQNDKK
CCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + L-lysine [Periplasm] = Proton [Cytoplasm] + L-lysine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1315732; 9278503; 7551055