Click here to switch to the map view.

The map label for this gene is pbpG

Identifier: 218695746

GI number: 218695746

Start: 2439438

End: 2440370

Strand: Reverse

Name: pbpG

Synonym: EC55989_2384

Alternate gene names: 218695746

Gene position: 2440370-2439438 (Counterclockwise)

Preceding gene: 218695747

Following gene: 218695744

Centisome position: 47.34

GC content: 51.02

Gene sequence:

>933_bases
ATGCCGAAATTTCGAGTTTCTTTATTTAGCCTGGCCCTGATGCTGGCTGTGCCTTTGGCACCGCAGGCCGTTGCGAAAAC
GGCAGCCGCTACCACCGCTTCACAACCGGAAATTGCCTCCGGTAGCGCGATGATTGTTGATCTGAATACCAACAAAGTGA
TCTATTCGAACCACCCGGATCTGGTGCGTCCGATTGCGTCTATCAGCAAATTAATGACCGCGATGGTTGTGCTGGATGCA
CGACTGCCGCTGGATGAAAAACTAAAAGTGGATATCAGCCAGACGCCGGAGATGAAAGGGGTCTATTCGCGCGTACGACT
GAATAGCGAAATCAGCCGTAAAGATATGCTGTTGCTGGCGCTGATGTCTTCAGAAAACCGCGCGGCGGCAAGCCTTGCGC
ACCATTATCCCGGTGGTTACAAAGCCTTTATTAAGGCAATGAATGCGAAAGCGAAATCGCTCGGAATGAACAACACGCGC
TTTGTTGAACCTACCGGATTGTCGGTGCATAACGTTTCAACTGCCCGTGACTTAACCAAACTGCTCATTGCCAGCAAACA
GTATCCGTTGATCGGGCAGTTAAGTACTACCCGGGAAGATATGGCAACCTTCTCTAACCCGACGTATACGCTGCCGTTCC
GCAATACTAATCATCTGGTGTATCGCGATAACTGGAATATTCAGTTAACCAAAACCGGCTTTACCAATGCGGCGGGCCAT
TGTCTGGTGATGCGTACGGTTATCAATAATAAACCGGTGGCGCTGGTAGTGATGGACGCGTTTGGCAAATATACCCATTT
TGCCGATGCCAGCCGCCTGCGTACCTGGATTGAAACCGGTAAAGTGATGCCTGTGCCGGCAGCAGCGTTGAGCTATAAAA
AACAAAAAGCCGCCCAAATGGCGGCGGCGGGGCAGACGGCACAGAACGATTAA

Upstream 100 bases:

>100_bases
TCGCACTTTTTGCTCGTTATGATGGCGTCGCTAGCCTCAGTAAATCCTTACTCCGGCGGTGCGCAACCCGTGCGCGTGAA
CCACTATCTGAATGCTCATC

Downstream 100 bases:

>100_bases
GTGAATTCGGATGGCGATACTCTGCCATCCGTAATTTTTACTCCACTTCCTGCCAGTTTTTCCGTTTACTGGTTTTACCG
ATTCCCGGATTCATGCTGTT

Product: D-alanyl-D-alanine endopeptidase

Products: NA

Alternate protein names: DD-endopeptidase; Penicillin-binding protein 7; PBP-7 [H]

Number of amino acids: Translated: 310; Mature: 309

Protein sequence:

>310_residues
MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDA
RLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNAAGH
CLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND

Sequences:

>Translated_310_residues
MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDA
RLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNAAGH
CLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND
>Mature_309_residues
PKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDAR
LPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTRF
VEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNAAGHC
LVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND

Specific function: Cell wall formation. May play a specialized role in remodeling the cell wall. Specifically hydrolyze the DD- diaminopimelate-alanine bonds in high-molecular-mass murein sacculi [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082054, Length=310, Percent_Identity=99.6774193548387, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1787062, Length=270, Percent_Identity=28.1481481481481, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082022, Length=275, Percent_Identity=25.4545454545455, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1786851, Length=264, Percent_Identity=26.5151515151515, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR018044
- InterPro:   IPR001967 [H]

Pfam domain/function: PF00768 Peptidase_S11 [H]

EC number: 3.4.99.- [C]

Molecular weight: Translated: 33854; Mature: 33723

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSNHPD
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCH
LVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLA
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHEEEEE
LMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTRFVEPTGLSVHNVSTARDLTK
EECCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCEEECCCCCEEECCHHHHHHHH
LLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNAAGH
HHHHCCCCCEEECCCCCHHHHHHCCCCEEEEECCCCCEEEEECCCEEEEEECCCCCCCCC
CLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQM
EEEEEEHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AAAGQTAQND
HHCCCCCCCC
>Mature Secondary Structure 
PKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAMIVDLNTNKVIYSNHPD
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCEEEECCCCH
LVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVYSRVRLNSEISRKDMLLLA
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHCCCCCCHHHEEEEE
LMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTRFVEPTGLSVHNVSTARDLTK
EECCCCHHHHHHHHHCCCHHHHHHHHHCCHHHHCCCCCCEEECCCCCEEECCHHHHHHHH
LLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNHLVYRDNWNIQLTKTGFTNAAGH
HHHHCCCCCEEECCCCCHHHHHHCCCCEEEEECCCCCEEEEECCCEEEEEECCCCCCCCC
CLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTWIETGKVMPVPAAALSYKKQKAAQM
EEEEEEHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
AAAGQTAQND
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 6386470; 7721700; 7925376 [H]