Click here to switch to the map view.

The map label for this gene is wcaA

Identifier: 218695682

GI number: 218695682

Start: 2362972

End: 2363811

Strand: Reverse

Name: wcaA

Synonym: EC55989_2315

Alternate gene names: 218695682

Gene position: 2363811-2362972 (Counterclockwise)

Preceding gene: 218695683

Following gene: 218695681

Centisome position: 45.86

GC content: 50.83

Gene sequence:

>840_bases
ATGAAAGACAATCCGCTGATTTCAATCTATATGCCGACCTGGAACCGCCAACAACTGGCGATTCGGGCGATAAAATCGGT
CCTGCGCCAGGACTACAGCAACTGGGAGATGATCATCGTGGATGATTGCTCCACCTCCTGGGAACAACTGCAACAGTACG
TCACTGCCCTCAACGATCCGCGTATTACCTACATTCACAACGACATTAACAGCGGGGCGTGCGCGGTACGTAACCAGGCG
ATTATGCTGGCGCAAGGGGAATACATTACCGGGATCGATGACGATGATGAATGGACGCCCAACCGCCTGAGCGTCTTCCT
CGCCCATAAACAGCAACTGGTCACTCACGCCTTTTTGTACGCTAACGACTACGTATGCCAGGGAGAAGTTTATTCCCAAC
CAGCAAGCTTACCACTGTATCCCAAATCGCCGTACTCACGCCGCCTGTTCTATAAACGCAATATCATTGGCAATCAGGTT
TTTACCTGGGCGTGGCGTTTCAAAGAGTGCTTGTTCGATACCGAACTGAAAGCAGCACAGGATTACGACATCTTCCTGCG
GATGGTGGTGGAGTATGGCGAACCGTGGAAAGTGGAAGAGGCGACGCAGATCCTGCATATCAATCACGGTGAAATGCAGA
TCACCTCGTCACCGAAAAAATTCTCCGGGTACTTCCACTTTTACCGCAAGCACAAAGATAAATTTGACCGTGCAAGCAAA
AAATATCAGCTGTTTACCCTCTACCAGATCCGCAATAAACGTATGACCTGGCGCACTTTGCTGACGCTGCTGTCGGTGCG
TAACGGCAAACGTCTGGCTGATGGTATTCGGGGGCGCTAA

Upstream 100 bases:

>100_bases
TCCGTAGGCCGGATAAGGCGCTCACGCCGCATCCGGCAACAGAACCCGTAAACAAGCTTATAAACCGGCAGAACATCTCG
ACACTCCGGGAGAACAACCT

Downstream 100 bases:

>100_bases
AGATGCTGGAAGATCTGCGTGCCAACAGCTGGAGTTTACGCCCGTGCTGCATGGTTCTTGCCTATCGTGTTGCTCATTTT
TGTTCGGTGTGGCGCAAAAA

Product: putative glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQA
IMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASK
KYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR

Sequences:

>Translated_279_residues
MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQA
IMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASK
KYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR
>Mature_279_residues
MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQA
IMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASK
KYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To R.meliloti exoO [H]

Homologues:

Organism=Escherichia coli, GI1788372, Length=279, Percent_Identity=99.6415770609319, Blast_Score=587, Evalue=1e-169,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001173 [H]

Pfam domain/function: PF00535 Glycos_transf_2 [H]

EC number: NA

Molecular weight: Translated: 33036; Mature: 33036

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDP
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
RITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLY
EEEEEECCCCCCCEEECCCEEEEECCCEEECCCCCCCCCCHHHEEEHHHHHHHHHHHHHH
ANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQVFTWAWRFKECLFDTELKAAQ
CCCEEECCHHHCCCCCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHHCCHHCCCC
DYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASK
CHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHCCC
KYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR
CEEEEEEEEHHCCCHHHHHHHHHHHHCCCCHHHHCCCCC
>Mature Secondary Structure
MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCSTSWEQLQQYVTALNDP
CCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCC
RITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNRLSVFLAHKQQLVTHAFLY
EEEEEECCCCCCCEEECCCEEEEECCCEEECCCCCCCCCCHHHEEEHHHHHHHHHHHHHH
ANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQVFTWAWRFKECLFDTELKAAQ
CCCEEECCHHHCCCCCCCCCCCCCCHHHHEEECCCCCCHHHHHHHHHHHHHHCCHHCCCC
DYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSPKKFSGYFHFYRKHKDKFDRASK
CHHHHHHHHHHCCCCCCCCCCEEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHCCC
KYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGIRGR
CEEEEEEEEHHCCCHHHHHHHHHHHHCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]