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The map label for this gene is cpsB

Identifier: 218695672

GI number: 218695672

Start: 2353497

End: 2354933

Strand: Reverse

Name: cpsB

Synonym: EC55989_2305

Alternate gene names: 218695672

Gene position: 2354933-2353497 (Counterclockwise)

Preceding gene: 218695673

Following gene: 218695671

Centisome position: 45.68

GC content: 55.25

Gene sequence:

>1437_bases
ATGGCGCAGTCGAAACTCTATCCAGTTGTGATGGCAGGTGGCTCCGGTAGCCGCTTATGGCCGCTTTCCCGCGTACTTTA
CCCCAAGCAGTTTTTATGCCTGAAAGGCGATCTCACCATGCTGCAAACCACCATCTGCCGCCTGAACGGCGTGGAGTGCG
AAAGCCCGGTGGTGATTTGCAATGAGCAGCACCGCTTTATTGTCGCGGAACAACTGCGTCAACTGAACAAACTCACCGAG
AACATTATTCTCGAACCGGCAGGGCGTAACACTGCACCTGCCATTGCACTGGCGGCGCTGGCGGCAAAACGTCATAGCCC
GGAGAGCGACCCGTTAATGTTGGTATTGGCGGCGGATCATGTGATTGCCGATGAAGACGCGTTCCGCGCCGCCGTACGTA
ACGCCATGCCGTATGCCGAAGCGGGCAAGCTGGTGACCTTTGGCATTGTGCCGGATCTACCAGAAACCGGTTATGGCTAT
ATTCGTCGCGGTGAAGTGTCTGCGGGTGAGCAGGATACGGTGGCCTTTGAAGTGGCGCAGTTTGTCGAAAAACCGAATCT
GGAAACCGCCCAGGCCTATGTGGCAAGCGGCGAATATTACTGGAACAGCGGTATGTTCCTGTTCCGCGCCGGACGCTATC
TCGAAGAACTGAAAAAATATCGTCCGGATATTCTCGATGCCTGTGAAAAAGCGATGAGCGCCGTCGATCCGGATCTCGAT
TTTATTCGTGTGGATGAAGATGCGTTTCTCGCCTGCCCGGAAGAGTCGGTGGATTACGCCGTCATGGAACGTACGGCAGA
TGCCGTTGTGGTGCCGATGGATGCGGGCTGGAGTGATGTCGGTTCCTGGTCCTCTTTATGGGAGATCAGCGCCCACACCG
CCGAGGGCAATGTTTGCCACGGCGATGTGATTAATCACAAAACCGAAAACAGCTATGTGTATGCCGAATCTGGCCTGGTC
ACCACCGTCGGGGTGAAAGATTTGGTGGTAGTGCAGACCAAAGATGCGGTGCTGATTGCTGACCGTAACGCGGTGCAGGA
TGTGAAAAAAGTGGTCGAGCAGATCAAAGCCGATGGTCGCCATGAGCATCGGGTGCATCGCGAAGTGTATCGTCCGTGGG
GCAAATATGACTCTATCGACGCGGGCGACCGCTACCAGGTGAAACGCATCACCGTGAAACCGGGCGAGGGCTTGTCGGTA
CAGATGCACCATCACCGCGCGGAACACTGGGTGGTTGTCGCGGGAACGGCAAAAGTCACTATCGACGGTGATATCAAACT
GCTTGGTGAAAACGAGTCCATTTATATTCCGCTGGGGGCGACGCATTGCCTGGAAAACCCGGGGAAAATTCCGCTCGATT
TAATTGAAGTGCGCTCCGGCTCTTATCTTGAAGAGGATGATGTGGTGCGCTTCGCGGATCGCTACGGACGGGTGTAA

Upstream 100 bases:

>100_bases
TGCTGCCCAAACACAACACGGTGGCACGTGAATATGCCGAACGCACGCTCGATAAAGAGAACGTGTTACGTCAATTTATA
AATGATATTCGGGGATAATT

Downstream 100 bases:

>100_bases
ACGTCGCATCAGGCGCTGTTTGTCGGATGCGGCGTGAACGCCTTATCCAACCTACGGTTCGGTTTTGTAGGCCTGATAAG
ACGCGGCAGCGTCGCATCAG

Product: mannose-1-phosphate guanyltransferase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTE
NIILEPAGRNTAPAIALAALAAKRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLD
FIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLV
TTVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSV
QMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV

Sequences:

>Translated_478_residues
MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTE
NIILEPAGRNTAPAIALAALAAKRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLD
FIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLV
TTVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSV
QMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV
>Mature_477_residues
AQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTEN
IILEPAGRNTAPAIALAALAAKRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGYI
RRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLDF
IRVDEDAFLACPEESVDYAVMERTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVT
TVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSVQ
MHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV

Specific function: Involved in the biosynthesis of the capsular polysaccharide colanic acid [H]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=478, Percent_Identity=99.3723849372385, Blast_Score=982, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 52974; Mature: 52842

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVIC
CCCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCEEEE
NEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAAKRHSPESDPLMLVLAADH
CCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCC
VIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGYIRRGEVSAGEQDTVAFEVAQ
EECCCHHHHHHHHHCCCCHHCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
FVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLD
HHHCCCCCHHHHEEECCEEEECCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
FIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCH
EEEECCCCEEECCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCHHEEEEEEECCCCCEEE
GDVINHKTENSYVYAESGLVTTVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGR
CHHHCCCCCCCEEEECCCEEEEECCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCC
HEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSVQMHHHRAEHWVVVAGTAKVT
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCEEEEEEEEEEEE
IDGDIKLLGENESIYIPLGATHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV
ECCCEEEEECCCEEEEECCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure 
AQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTTICRLNGVECESPVVIC
CCCCEEEEEEECCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCEEEE
NEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAAKRHSPESDPLMLVLAADH
CCCCCCHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCC
VIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGYIRRGEVSAGEQDTVAFEVAQ
EECCCHHHHHHHHHCCCCHHCCCEEEEECCCCCCCCCCCEEEECCCCCCCCCHHHHHHHH
FVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELKKYRPDILDACEKAMSAVDPDLD
HHHCCCCCHHHHEEECCEEEECCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCC
FIRVDEDAFLACPEESVDYAVMERTADAVVVPMDAGWSDVGSWSSLWEISAHTAEGNVCH
EEEECCCCEEECCCCCCCHHHHHCCCCEEEEEECCCCCCCCCCHHEEEEEEECCCCCEEE
GDVINHKTENSYVYAESGLVTTVGVKDLVVVQTKDAVLIADRNAVQDVKKVVEQIKADGR
CHHHCCCCCCCEEEECCCEEEEECCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHCCCC
HEHRVHREVYRPWGKYDSIDAGDRYQVKRITVKPGEGLSVQMHHHRAEHWVVVAGTAKVT
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCEEEEEEEEEEEE
IDGDIKLLGENESIYIPLGATHCLENPGKIPLDLIEVRSGSYLEEDDVVRFADRYGRV
ECCCEEEEECCCEEEEECCHHHHHCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1712067; 11677609 [H]