Click here to switch to the map view.

The map label for this gene is hisB

Identifier: 218695648

GI number: 218695648

Start: 2324495

End: 2325562

Strand: Direct

Name: hisB

Synonym: EC55989_2281

Alternate gene names: 218695648

Gene position: 2324495-2325562 (Clockwise)

Preceding gene: 218695647

Following gene: 218695649

Centisome position: 45.09

GC content: 53.93

Gene sequence:

>1068_bases
ATGAGTCAGAAGTATCTTTTTATCGATCGCGATGGAACCCTGATTAGCGAACCGCCGAGTGATTTTCAGGTGGACCGTTT
TGACAAACTCGCCTTTGAACCGGGCGTGATCCCGGAACTGCTGAAGCTGCAAAAAGCGGGCTACAAGCTGGTGATGATCA
CTAATCAGGATGGTCTGGGAACACAAAGTTTCCCGCAGGCGGATTTTGATGGCCCGCACAACCTGATGATGCAGATCTTC
ACCTCGCAAGGCGTGCAGTTTGATGAAGTGCTGATTTGTCCGCACCTGCCCGCCGATGAGTGCGACTGCCGTAAGCCGAA
AGTAAAACTGGTAGAGCGTTATCTGGCTGAGCAAGCGATGGATCGTGCCAACAGTTATGTGATTGGCGATCGCGCGACCG
ACATTCAACTGGCGGAAAACATGGGTATTAATGGTTTACGCTACGACCGCGAAATCCTGAGCTGGCCGATGATTGGCGAG
CAACTCACTAAACGAGACCGTTACGCCCATGTAGTGCGCAACACCAAAGAGACGCAAATTGACGTCCAGGTGTGGCTGGA
TCGCGAAGGTGGCAGCAAGATTAATACCGGCGTTGGCTTCTTTGATCACATGCTGGATCAGATCGCCACCCACGGCGGTT
TCCGTATGGAAATCAACGTCAAAGGCGACCTCTATATCGACGATCACCACACCGTCGAAGATACCGGCCTGGCGCTGGGC
GAAGCGCTAAAAATCGCCCTTGGCGACAAACGCGGTATTTGCCGCTTTGGTTTTGTGCTACCGATGGACGAATGCCTTGC
CCGCTGCGCGCTGGATATCTCTGGTCGCCCGCACCTGGAATATAAAGCCGAGTTTACCTACCAGCGCGTGGGCGATCTCA
GCACCGAAATGATCGAGCACTTCTTCCGTTCGCTCTCATACACCATGGGCGTGACGCTACACCTGAAAACCAAAGGTAAA
AACGATCACCACCGTGTAGAGAGCCTGTTCAAAGCCTTTGGTCGCACCCTGCGCCAGGCCATCCGCGTGGAAGGCGACAC
CCTGCCCTCGTCGAAAGGAGTGCTGTAA

Upstream 100 bases:

>100_bases
AGAATAAACAACCCTCTTTAAGCGGCTGCCTGCGAATTACCGTCGGAACCCGTGAAGAAAGCCAGCGCGTCATTGACGCC
TTACGTGCGGAGCAAGTTTG

Downstream 100 bases:

>100_bases
TGAACGTGGTGATCCTTGATACCGGCTGCGCCAATCTGAACTCGGTGAAGTCAGCCATTGCGCGCCACGGTTATGAACCA
AAAGTCAGCCGTGACCCGGA

Product: imidazole glycerol-phosphate dehydratase/histidinol phosphatase

Products: NA

Alternate protein names: Histidinol-phosphatase; Imidazoleglycerol-phosphate dehydratase; IGPD [H]

Number of amino acids: Translated: 355; Mature: 354

Protein sequence:

>355_residues
MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQKAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIF
TSQGVQFDEVLICPHLPADECDCRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILSWPMIGE
QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEINVKGDLYIDDHHTVEDTGLALG
EALKIALGDKRGICRFGFVLPMDECLARCALDISGRPHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGK
NDHHRVESLFKAFGRTLRQAIRVEGDTLPSSKGVL

Sequences:

>Translated_355_residues
MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQKAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIF
TSQGVQFDEVLICPHLPADECDCRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILSWPMIGE
QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEINVKGDLYIDDHHTVEDTGLALG
EALKIALGDKRGICRFGFVLPMDECLARCALDISGRPHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGK
NDHHRVESLFKAFGRTLRQAIRVEGDTLPSSKGVL
>Mature_354_residues
SQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQKAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFT
SQGVQFDEVLICPHLPADECDCRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILSWPMIGEQ
LTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEINVKGDLYIDDHHTVEDTGLALGE
ALKIALGDKRGICRFGFVLPMDECLARCALDISGRPHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKN
DHHRVESLFKAFGRTLRQAIRVEGDTLPSSKGVL

Specific function: Histidine biosynthesis; sixth step. Histidine biosynthesis; eighth step. [C]

COG id: COG0131

COG function: function code E; Imidazoleglycerol-phosphate dehydratase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the imidazoleglycerol-phosphate dehydratase family [H]

Homologues:

Organism=Escherichia coli, GI87082027, Length=355, Percent_Identity=98.8732394366197, Blast_Score=731, Evalue=0.0,
Organism=Escherichia coli, GI1786399, Length=150, Percent_Identity=32.6666666666667, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324776, Length=166, Percent_Identity=47.5903614457831, Blast_Score=159, Evalue=9e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006549
- InterPro:   IPR020566
- InterPro:   IPR005954
- InterPro:   IPR006543
- InterPro:   IPR000807
- InterPro:   IPR020565
- InterPro:   IPR013954
- InterPro:   IPR020568 [H]

Pfam domain/function: PF00475 IGPD; PF08645 PNK3P [H]

EC number: =3.1.3.15; =4.2.1.19 [H]

Molecular weight: Translated: 40249; Mature: 40117

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00954 IGP_DEHYDRATASE_1 ; PS00955 IGP_DEHYDRATASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQKAGYKLVMITNQDGLG
CCCCEEEEECCCCEECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCEEEEEEECCCCCC
TQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECDCRKPKVKLVERYLAEQAM
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
DRANSYVIGDRATDIQLAENMGINGLRYDREILSWPMIGEQLTKRDRYAHVVRNTKETQI
HHCCCEEECCCCCCEEEHHCCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEE
DVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEINVKGDLYIDDHHTVEDTGLALG
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCHHHHHHHH
EALKIALGDKRGICRFGFVLPMDECLARCALDISGRPHLEYKAEFTYQRVGDLSTEMIEH
HHEEEEECCCCCCEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHCHHHHHHHH
FFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFGRTLRQAIRVEGDTLPSSKGVL
HHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQKAGYKLVMITNQDGLG
CCCEEEEECCCCEECCCCCCCCCCHHHHHCCCCCCCHHHHHHHHCCEEEEEEECCCCCC
TQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECDCRKPKVKLVERYLAEQAM
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHH
DRANSYVIGDRATDIQLAENMGINGLRYDREILSWPMIGEQLTKRDRYAHVVRNTKETQI
HHCCCEEECCCCCCEEEHHCCCCCCEEHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEE
DVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEINVKGDLYIDDHHTVEDTGLALG
EEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCEEEECCCCCCHHHHHHHH
EALKIALGDKRGICRFGFVLPMDECLARCALDISGRPHLEYKAEFTYQRVGDLSTEMIEH
HHEEEEECCCCCCEEECCCCCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHCHHHHHHHH
FFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFGRTLRQAIRVEGDTLPSSKGVL
HHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10222209; 11206551; 11258796 [H]