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The map label for this gene is irp

Identifier: 218695590

GI number: 218695590

Start: 2259594

End: 2261171

Strand: Direct

Name: irp

Synonym: EC55989_2211

Alternate gene names: 218695590

Gene position: 2259594-2261171 (Clockwise)

Preceding gene: 218695589

Following gene: 218695591

Centisome position: 43.83

GC content: 58.75

Gene sequence:

>1578_bases
ATGAATTCTTCCTTTGAATCTCTGATTGAACAGTATCCCTTACCCATTGCCGAACAGTTGCGCCACTGGGCGGCCCGTTA
TGCCTCGCGAATTGCCGTCGTTGATGCAAAGGGGTCGTTAACCTACAGCGCGCTTGATGCACAAGTTGACGAACTTGCCG
CAGGTCTGTCATCACTGGGTTTGCGTTCGGGGGAGCATGTAATTGTGCAGCTTCCCAACGACAACGCGTTTGTTACCCTG
CTGTTCGCCTTGTTAAGACTGGGCGTTATCCCCGTGCTGGCGATGCCCTCGCAACGGGCGCTGGATATCGACGCGCTGAT
TGAGCTGGCGCAACCCGTCGCTTACGTTATTCACGGGGAAAACCACGCAGAGCTGGCCCGACAGATGGCGCACAAACACG
CCTGCTTGCGTCATGTTCTGGTCGCTGGAGAGACCATGAGCGACGATTTTACGCCGCTCTTCTCCCTTCACGGTGAGCGA
CAGGCATGGCCGCAGCCTGATGTTTCCACCACCGCGTTGTTGTTGCTCTCAGGCGGCACAACCGGCACGCCCAAACTCAT
CCCGCGCCGACATGCCGACTATAGCTATAACTTCAGCGCTTCTGCTGAACTGTGCGGCATCAGCCAACAGAGCGTGTATC
TCGCCGTCCTCCCGGTGGCGCATAACTTTCCGCTGGCCTGCCCCGGTATTCTGGGAACGCTTGCCTGCGGCGGAAAAGTG
GTGCTGACCGACAGCGCCAGCTGTGATGAGGTGATGCCTTTAATCGCGCAGGAAAGAGTGACTCACGTCGCCCTGGTTCC
GGCGCTGGCGCAATTATGGGTGCAGGCCAGGGAGTGGGAAGACAGCGACCTTTCGTCGCTGCGCGTCATTCAGGCAGGCG
GCGCCCGGCTCGACCCGACGCTTGCTGAGCAGGTTATCGCCACCTTTGACTGTACCCTGCAACAGGTTTTCGGTATGGCG
GAAGGCCTGCTCTGTTTTACCCGGCTGGACGATCCGCATGCCACCATTCTCCACAGCCAGGGGCGCCCGTTGTCCCCTCT
GGATGAAATCCGCATCGTTGATCAAGACGAGAACGACGTCGCGCCGGGCGAAACCGGGCAATTGTTAACGCGCGGCCCTT
ATACCATTTCGGGCTATTACCGCGCCCCTGCCCACAACGCGCAGGCCTTTACCGCGCAAGGGTTTTACCGCACAGGCGAC
AATGTCAGGCTGGATGAGGTGGGGAACCTGCACGTTGAGGGACGCATAAAAGAGCAGATCAACCGCGCCGGAGAAAAAAT
AGCCGCGGCTGAAGTGGAATCGGCACTGCTGCGTTTAGCGGAAGTGCAAGATTGCGCGGTGGTCGCCGCGCCGGACACGC
TGCTTGGCGAGCGGATTTGCGCGTTTATCATCGCGCAGCAGGTGCCAACTGACTATCAGCAGTTGCGTCAACAACTGACC
CGTATGGGGCTCAGCGCGTGGAAAATTCCTGACCAAATCGAGTTTCTGGACCACTGGCCGCTCACCGCCGTCGGCAAGAT
AGACAAAAAACGCCTGACGGCTCTCGCCGTCGACCGTTATCGCCATTCTGCCCAATAA

Upstream 100 bases:

>100_bases
AGCCCGGTCCTTTTTTACGCAGATTGTCCGCCATTTTCCCCACGCATTTTCTGCAATGACCGCGTGGCAAAAACAGCCCA
GTACTTCAGAAAGGTGACGC

Downstream 100 bases:

>100_bases
GCGCAAACCGACCCGAAACAGGTTGAAATAAACCCGTTTCGGGTAGCACTACTATTAGAAATAGTTATCATTTTCAATTC
ACCATTGTCGGTATTTTTGG

Product: salicyl-AMP ligase YbtE

Products: (2,3-Dihydroxybenzoyl)adenylate; Diphosphate [C]

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSALDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTL
LFALLRLGVIPVLAMPSQRALDIDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
QAWPQPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHNFPLACPGILGTLACGGKV
VLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMA
EGLLCFTRLDDPHATILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFYRTGD
NVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTLLGERICAFIIAQQVPTDYQQLRQQLT
RMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRLTALAVDRYRHSAQ

Sequences:

>Translated_525_residues
MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSALDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTL
LFALLRLGVIPVLAMPSQRALDIDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
QAWPQPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHNFPLACPGILGTLACGGKV
VLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMA
EGLLCFTRLDDPHATILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFYRTGD
NVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTLLGERICAFIIAQQVPTDYQQLRQQLT
RMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRLTALAVDRYRHSAQ
>Mature_525_residues
MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSALDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTL
LFALLRLGVIPVLAMPSQRALDIDALIELAQPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
QAWPQPDVSTTALLLLSGGTTGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHNFPLACPGILGTLACGGKV
VLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMA
EGLLCFTRLDDPHATILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFYRTGD
NVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTLLGERICAFIIAQQVPTDYQQLRQQLT
RMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRLTALAVDRYRHSAQ

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=534, Percent_Identity=23.5955056179775, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI187761343, Length=534, Percent_Identity=23.5955056179775, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI156151445, Length=511, Percent_Identity=24.6575342465753, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI38505220, Length=407, Percent_Identity=24.5700245700246, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI115511026, Length=477, Percent_Identity=23.6897274633124, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI122937307, Length=470, Percent_Identity=24.0425531914894, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI157311624, Length=532, Percent_Identity=23.8721804511278, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI157311622, Length=532, Percent_Identity=23.8721804511278, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI58082049, Length=528, Percent_Identity=23.1060606060606, Blast_Score=93, Evalue=7e-19,
Organism=Homo sapiens, GI42544132, Length=489, Percent_Identity=22.9038854805726, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI195947378, Length=411, Percent_Identity=23.3576642335766, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI28416953, Length=522, Percent_Identity=23.1800766283525, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI1786810, Length=507, Percent_Identity=42.9980276134122, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI145693145, Length=516, Percent_Identity=27.7131782945736, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1788107, Length=533, Percent_Identity=25.328330206379, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI221142682, Length=484, Percent_Identity=23.9669421487603, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1789201, Length=350, Percent_Identity=26.2857142857143, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786529, Length=528, Percent_Identity=23.4848484848485, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790505, Length=371, Percent_Identity=24.2587601078167, Blast_Score=76, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=533, Percent_Identity=27.9549718574109, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=541, Percent_Identity=26.8022181146026, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=547, Percent_Identity=24.4972577696526, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17558820, Length=503, Percent_Identity=23.6580516898608, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71994703, Length=533, Percent_Identity=24.015009380863, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71994690, Length=533, Percent_Identity=24.015009380863, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71994694, Length=533, Percent_Identity=24.015009380863, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17531443, Length=376, Percent_Identity=25.7978723404255, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71985884, Length=365, Percent_Identity=27.6712328767123, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=384, Percent_Identity=27.6041666666667, Blast_Score=100, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=502, Percent_Identity=21.7131474103586, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17556356, Length=357, Percent_Identity=26.0504201680672, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=243, Percent_Identity=29.6296296296296, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71982997, Length=535, Percent_Identity=24.4859813084112, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71983001, Length=535, Percent_Identity=24.4859813084112, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=367, Percent_Identity=25.6130790190736, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6323182, Length=535, Percent_Identity=22.2429906542056, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6319264, Length=132, Percent_Identity=28.030303030303, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24581924, Length=533, Percent_Identity=24.765478424015, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355181, Length=509, Percent_Identity=24.3614931237721, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI18859661, Length=376, Percent_Identity=25.2659574468085, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI19922652, Length=515, Percent_Identity=22.1359223300971, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI62472339, Length=542, Percent_Identity=23.0627306273063, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24667955, Length=542, Percent_Identity=23.0627306273063, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24656500, Length=510, Percent_Identity=21.9607843137255, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21356947, Length=362, Percent_Identity=23.2044198895028, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI21356441, Length=480, Percent_Identity=22.5, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24667959, Length=378, Percent_Identity=25.3968253968254, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24648253, Length=476, Percent_Identity=22.6890756302521, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI20130357, Length=520, Percent_Identity=22.3076923076923, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648260, Length=351, Percent_Identity=21.9373219373219, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58; 2.3.1.- [C]

Molecular weight: Translated: 57286; Mature: 57286

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSALDAQVDELAAGLSSLG
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHC
LRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALDIDALIELAQPVAYVIHGE
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEEEEECC
NHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGERQAWPQPDVSTTALLLLSGGT
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEEECCC
TGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHNFPLACPGILGTLACGGKV
CCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCEE
VLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWEDSDLSSLRVIQAGGARLDPT
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCCCHH
LAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHATILHSQGRPLSPLDEIRIVDQDENDV
HHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEECCCCCCCCHHHEEEEECCCCCC
APGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFYRTGDNVRLDEVGNLHVEGRIKEQI
CCCCCCCEEECCCEEEEEEEECCCCCCCEEEECCEEECCCCEEECCCCCEEECCHHHHHH
NRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTLLGERICAFIIAQQVPTDYQQLRQQLT
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRLTALAVDRYRHSAQ
HCCCHHCCCCCHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSALDAQVDELAAGLSSLG
CCCHHHHHHHHCCCCHHHHHHHHHHHHHCEEEEEECCCCEEHHHHHHHHHHHHHHHHHHC
LRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALDIDALIELAQPVAYVIHGE
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCEEEEEECC
NHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGERQAWPQPDVSTTALLLLSGGT
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCEEEEEEECCC
TGTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLPVAHNFPLACPGILGTLACGGKV
CCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHCCCEE
VLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQAREWEDSDLSSLRVIQAGGARLDPT
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEECCCCCCCHH
LAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHATILHSQGRPLSPLDEIRIVDQDENDV
HHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEECCCCCCCCHHHEEEEECCCCCC
APGETGQLLTRGPYTISGYYRAPAHNAQAFTAQGFYRTGDNVRLDEVGNLHVEGRIKEQI
CCCCCCCEEECCCEEEEEEEECCCCCCCEEEECCEEECCCCEEECCCCCEEECCHHHHHH
NRAGEKIAAAEVESALLRLAEVQDCAVVAAPDTLLGERICAFIIAQQVPTDYQQLRQQLT
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
RMGLSAWKIPDQIEFLDHWPLTAVGKIDKKRLTALAVDRYRHSAQ
HCCCHHCCCCCHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,3-Dihydroxybenzoate; ATP [C]

Specific reaction: 2,3-Dihydroxybenzoate + ATP <==> (2,3-Dihydroxybenzoyl)adenylate + Diphosphate [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]