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The map label for this gene is yedX

Identifier: 218695575

GI number: 218695575

Start: 2227981

End: 2228394

Strand: Direct

Name: yedX

Synonym: EC55989_2196

Alternate gene names: 218695575

Gene position: 2227981-2228394 (Clockwise)

Preceding gene: 218695572

Following gene: 218695576

Centisome position: 43.22

GC content: 41.55

Gene sequence:

>414_bases
ATGTTAAAGCGTTATTTAGTACTCTCCGTAGTAACGGCTGCATTTTCATTACCTTCTTTGGTCTATGCTGCACAACAAAA
CATTCTTAGTGTGCACATTTTGAACCAGCAAACAGGAAAACCCGCTGCCGACGTGACAGTCACTCTTGAAAAGAAAGCGG
ACAACGGCTGGTTACAACTTAATACCGCCAAAACAGATAAGGATGGACGAATTAAGGCACTGTGGCCCGAGCAAACTGCA
ACTACGGGCGATTACCGTGTCGTATTTAAAACCGGGGACTATTTCAAGAAACAAAATCTTGAAAGTTTCTTCCCTGAGAT
CCCCGTTGAATTTCATATTAATAAAGTGAACGAGCATTATCATGTGCCTTTATTACTTAGCCAATATGGGTATTCAACCT
ATCGTGGCAGTTAA

Upstream 100 bases:

>100_bases
AAACAGCCTGGTTCATTACAAAATTGTAATGCTGCTGTAAGGTTACCGTGGACGCTTTTTCGCTATCCTCAAAACTCATT
CACATGACAAGGATATAAAC

Downstream 100 bases:

>100_bases
TGCATAAATATGTAAAAGCAGATCTCTGCTACTGTTAGGGAGGACACTCCCTGACAGATTAACAGTAAACGGCTCTTGCT
GGCTAACGACAAAAAAGTGT

Product: hypothetical protein

Products: NA

Alternate protein names: HIU hydrolase; HIUHase; Transthyretin-like protein; TLP

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAADVTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTA
TTGDYRVVFKTGDYFKKQNLESFFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS

Sequences:

>Translated_137_residues
MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAADVTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTA
TTGDYRVVFKTGDYFKKQNLESFFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS
>Mature_137_residues
MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAADVTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTA
TTGDYRVVFKTGDYFKKQNLESFFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS

Specific function: Catalyzes the hydrolysis of 5-hydroxyisourate (HIU) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (OHCU)

COG id: COG2351

COG function: function code R; Transthyretin-like protein

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transthyretin family. 5-hydroxyisourate hydrolase subfamily

Homologues:

Organism=Escherichia coli, GI1788281, Length=137, Percent_Identity=98.5401459854015, Blast_Score=280, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI115534555, Length=136, Percent_Identity=35.2941176470588, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI115532920, Length=109, Percent_Identity=40.3669724770642, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115532922, Length=109, Percent_Identity=40.3669724770642, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24652571, Length=110, Percent_Identity=40, Blast_Score=85, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIUH_ECO57 (Q8XB75)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C85815
- PIR:   D90967
- RefSeq:   NP_288429.1
- RefSeq:   NP_310735.1
- ProteinModelPortal:   Q8XB75
- SMR:   Q8XB75
- EnsemblBacteria:   EBESCT00000027088
- EnsemblBacteria:   EBESCT00000056546
- GeneID:   912669
- GeneID:   961974
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3062
- KEGG:   ecs:ECs2708
- GeneTree:   EBGT00050000010741
- HOGENOM:   HBG445217
- OMA:   GNPLSVH
- ProtClustDB:   PRK15036
- BioCyc:   ECOL83334:ECS2708-MONOMER
- GO:   GO:0006810
- InterPro:   IPR014306
- InterPro:   IPR000895
- Gene3D:   G3DSA:2.60.40.180
- PRINTS:   PR00189
- SMART:   SM00095
- TIGRFAMs:   TIGR02962

Pfam domain/function: PF00576 Transthyretin; SSF49472 Transthyretin

EC number: =3.5.2.17

Molecular weight: Translated: 15537; Mature: 15537

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS00768 TRANSTHYRETIN_1; PS00769 TRANSTHYRETIN_2

Important sites: BINDING 32-32 BINDING 70-70 BINDING 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAADVTVTLEKKADNGWLQL
CCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEEECCCCCEEEE
NTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLESFFPEIPVEFHINKVNEHY
EECCCCCCCCEEEECCCCCCCCCCEEEEEECCCCHHHCCHHHHCCCCCEEEEEEECCCEE
HVPLLLSQYGYSTYRGS
ECEEEEECCCCCCCCCC
>Mature Secondary Structure
MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAADVTVTLEKKADNGWLQL
CCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEEEEEECCCCCEEEE
NTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLESFFPEIPVEFHINKVNEHY
EECCCCCCCCEEEECCCCCCCCCCEEEEEECCCCHHHCCHHHHCCCCCEEEEEEECCCEE
HVPLLLSQYGYSTYRGS
ECEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796