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The map label for this gene is emrE

Identifier: 218695541

GI number: 218695541

Start: 2198063

End: 2198575

Strand: Reverse

Name: emrE

Synonym: EC55989_2156

Alternate gene names: 218695541

Gene position: 2198575-2198063 (Counterclockwise)

Preceding gene: 218695542

Following gene: 218695540

Centisome position: 42.65

GC content: 44.25

Gene sequence:

>513_bases
TTGCTCCCAGGAAGAGTGAACTCATTCGTTAGCCTGGGGTTCCTTCTGATCATTATTGTTCCTGCCTTTATCTCTTGCCA
CGCCAGAGCCCCCTGGATACACATACACCAGGATGAAAACGGTGAGTTGTGTTCAAACTGTAGTACAATTCTCTCCAGTA
TGAACAGGAAAGAATATGCTATGAACCCTTATATTTATCTTGGTGGCGCAATACTTGCAGAGGTCATTGGTACAACCTTA
ATGAAGTTTTCAGAGGGGTTTACACGGTTATGGCCCTCTGTTGGCACAATTATTTGCTATTGTGCATCATTCTGGTTATT
AGCTCAGACGCTGGCTTATATACCTACAGGGATTGCTTATGCTATCTGGTCAGGCGTCGGTATCGTTCTGATTAGTTTGC
TTTCCTGGGGATTTTTTGGTCAACGACTGGACCTGCCAGCTATCATAGGCATGATGTTGATTTGTGCCGGTGTGTTAGTC
ATTAACTTGTTGTCACGAAGTGCACCGCATTAA

Upstream 100 bases:

>100_bases
GGGCAAACTCACTCAATTTCTGGTTTAAAATGGATACCTGATCCTGAAAGTTTTCTGCCATCCACGATCCGTAAACGCGA
TAACATGTTTTGTCTTCGCT

Downstream 100 bases:

>100_bases
AAAATAATTATCCTTGAGATAACAGGAATCTGATTTGTGCATATTATGGCTAATGTTCGGGGCACCTTTATAATTATGAT
TGCATTTGCGCGATCATTGC

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Efflux-multidrug resistance protein emrE; Ethidium resistance protein; Methyl viologen resistance protein C [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MLPGRVNSFVSLGFLLIIIVPAFISCHARAPWIHIHQDENGELCSNCSTILSSMNRKEYAMNPYIYLGGAILAEVIGTTL
MKFSEGFTRLWPSVGTIICYCASFWLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLV
INLLSRSAPH

Sequences:

>Translated_170_residues
MLPGRVNSFVSLGFLLIIIVPAFISCHARAPWIHIHQDENGELCSNCSTILSSMNRKEYAMNPYIYLGGAILAEVIGTTL
MKFSEGFTRLWPSVGTIICYCASFWLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLV
INLLSRSAPH
>Mature_170_residues
MLPGRVNSFVSLGFLLIIIVPAFISCHARAPWIHIHQDENGELCSNCSTILSSMNRKEYAMNPYIYLGGAILAEVIGTTL
MKFSEGFTRLWPSVGTIICYCASFWLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLV
INLLSRSAPH

Specific function: Multidrug transporter that expels positively charged hydrophobic drugs across the inner membrane of E.coli., thereby conferring resistance to a wide range of toxic compounds. The drug efflux is coupled to an influx of protons. Is involved in the resistanc

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=110, Percent_Identity=98.1818181818182, Blast_Score=217, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 18598; Mature: 18598

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPGRVNSFVSLGFLLIIIVPAFISCHARAPWIHIHQDENGELCSNCSTILSSMNRKEYA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHH
MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASFWLLAQTLAYIPTGIAY
CCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVINLLSRSAPH
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLPGRVNSFVSLGFLLIIIVPAFISCHARAPWIHIHQDENGELCSNCSTILSSMNRKEYA
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCHHHH
MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASFWLLAQTLAYIPTGIAY
CCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVINLLSRSAPH
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1936950; 1320256; 9278503; 7896833; 9050242; 9688273 [H]