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The map label for this gene is yebK

Identifier: 218695419

GI number: 218695419

Start: 2087835

End: 2088704

Strand: Direct

Name: yebK

Synonym: EC55989_2032

Alternate gene names: 218695419

Gene position: 2087835-2088704 (Clockwise)

Preceding gene: 218695415

Following gene: 218695420

Centisome position: 40.5

GC content: 48.97

Gene sequence:

>870_bases
ATGAATATGCTGGAAAAAATCCAGTCTCAGCTGGAACATTTGAGCAAATCAGAGCGCAAAGTTGCCGAGGTCATTCTGGC
TTCGCCCGATAACGCGATCCATTCGAGTATTGCTGCTATGGCACTGGAAGCCAATGTTAGCGAACCGACGGTGAATCGTT
TCTGTCGCAGCATGGACACGCGCGGTTTTCCTGATTTTAAACTTCATCTGGCACAGAGTCTGGCGAATGGCACTCCCTAT
GTTAATCGCAATGTCAATGAAGATGACAGCGTTGAATCATACACAGGGAAAATATTTGAGTCCGCAATGGCAACGCTTGA
TCATGTCCGTCATTCACTGGATAAATCTGCCATCAACCGCGCCGTCGACTTGCTCACTCAGGCAAAAAAAATCGCCTTTT
TCGGATTAGGCTCTTCAGCCGCCGTTGCCCACGATGCGATGAATAAGTTCTTTCGTTTTAATGTTCCGGTGGTGTACTCC
GATGATATCGTGCTGCAACGCATGAGTTGTATGAATTGTAGCGACGGAGACGTGGTGGTGCTGATTTCTCACACTGGAAG
AACAAAAAATCTGGTCGAGCTGGCGCAGCTGGCTCGCGAAAACGACGCAATGGTGATTGCCCTCACCTCTGCGGGTACCC
CGCTCGCCCGGGAAGCAACGCTGGCAATTACCCTCGACGTACCGGAAGATACTGACATTTATATGCCCATGGTTTCTCGA
CTTGCACAGCTGACCGTGATAGATGTGCTGGCGACAGGATTTACTTTGCGACGCGGTGCAAAATTCAGAGATAACTTGAA
GCGGGTCAAGGAAGCGCTGAAGGAATCGCGTTTTGATAAGCAGTTACTTAATTTAAGTGACGATCGCTAA

Upstream 100 bases:

>100_bases
TTTTGTCATTAACACGGCACACAGGCGTAATATCTGACAAAACTGCATTTCGATTTCTTTCAGTGCGGAAATCGTCATTA
CCCGTGAGTCTCTTTACATC

Downstream 100 bases:

>100_bases
AAACGACTGTCACTGTCCTAATCTTATACGACATCCGAATGAGATTAATTTATCGCCATCGCGGCGTTATTTCATTCGGA
TTTCATGTTCAAGCAACACC

Product: DNA-binding transcriptional regulator HexR

Products: NA

Alternate protein names: Hex regulon repressor

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEANVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPY
VNRNVNEDDSVESYTGKIFESAMATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLAREATLAITLDVPEDTDIYMPMVSR
LAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKESRFDKQLLNLSDDR

Sequences:

>Translated_289_residues
MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEANVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPY
VNRNVNEDDSVESYTGKIFESAMATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLAREATLAITLDVPEDTDIYMPMVSR
LAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKESRFDKQLLNLSDDR
>Mature_289_residues
MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEANVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPY
VNRNVNEDDSVESYTGKIFESAMATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLAREATLAITLDVPEDTDIYMPMVSR
LAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKESRFDKQLLNLSDDR

Specific function: Represses the expression of the hex regulon (zwf, eda, glp and gap)

COG id: COG1737

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SIS domain

Homologues:

Organism=Escherichia coli, GI1788159, Length=289, Percent_Identity=100, Blast_Score=592, Evalue=1e-171,
Organism=Escherichia coli, GI1788767, Length=264, Percent_Identity=27.6515151515151, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082369, Length=240, Percent_Identity=24.5833333333333, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87082130, Length=195, Percent_Identity=27.1794871794872, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): HEXR_ECOLI (P46118)

Other databases:

- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   M55005
- EMBL:   U27210
- PIR:   E64947
- RefSeq:   AP_002473.1
- RefSeq:   NP_416367.1
- ProteinModelPortal:   P46118
- DIP:   DIP-9891N
- STRING:   P46118
- EnsemblBacteria:   EBESCT00000000090
- EnsemblBacteria:   EBESCT00000014825
- GeneID:   946373
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1842
- KEGG:   eco:b1853
- EchoBASE:   EB2702
- EcoGene:   EG12860
- eggNOG:   COG1737
- GeneTree:   EBGT00050000010596
- HOGENOM:   HBG725281
- OMA:   LNASRYP
- ProtClustDB:   PRK11302
- BioCyc:   EcoCyc:EG12860-MONOMER
- Genevestigator:   P46118
- InterPro:   IPR000281
- InterPro:   IPR001347
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF01418 HTH_6; PF01380 SIS

EC number: NA

Molecular weight: Translated: 31976; Mature: 31976

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS51071 HTH_RPIR; PS51464 SIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEANVSEPTVNRFCRSMDT
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
RGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESAMATLDHVRHSLDKSAINR
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYSDDIVLQRMSCMNCSDGDVVV
HHHHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCCCCEEE
LISHTGRTKNLVELAQLARENDAMVIALTSAGTPLAREATLAITLDVPEDTDIYMPMVSR
EEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHH
LAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKESRFDKQLLNLSDDR
HHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEANVSEPTVNRFCRSMDT
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCC
RGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESAMATLDHVRHSLDKSAINR
CCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYSDDIVLQRMSCMNCSDGDVVV
HHHHHHHHHHHHEEECCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHCCCCCCCEEE
LISHTGRTKNLVELAQLARENDAMVIALTSAGTPLAREATLAITLDVPEDTDIYMPMVSR
EEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHH
LAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKESRFDKQLLNLSDDR
HHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1704005; 7567469