Click here to switch to the map view.

The map label for this gene is yebU

Identifier: 218695401

GI number: 218695401

Start: 2071406

End: 2072845

Strand: Direct

Name: yebU

Synonym: EC55989_2012

Alternate gene names: 218695401

Gene position: 2071406-2072845 (Clockwise)

Preceding gene: 218695400

Following gene: 218695402

Centisome position: 40.18

GC content: 52.36

Gene sequence:

>1440_bases
GTGGCCCAACACACCGTTTATTTCCCGGACGCCTTTCTGACACAAATGCGCGAAGCGATGCCTTCGACGCTCTCATTTGA
TGATTTTCTTGCCGCCTGTCAGCGCCCGTTGCGCCGCAGCATTCGCGTTAATACGCTGAAAATCTCCGTTGCTGATTTCC
TGCAATTAACCGCTCCTTATGGCTGGACGCTTACGCCAATTCCGTGGTGTGAAGAAGGTTTCTGGATTGAACGCGACAAT
GAAGATGCATTGCCATTGGGTAGTACCGCCGAGCATTTAAGCGGCCTGTTTTATATTCAGGAAGCCAGTTCAATGTTGCC
CGTTGCCGCCTTGTTTGCTGACGATAATGCACCACAGCGGGTGATGGATGTCGCAGCTGCGCCAGGCTCCAAAACGACGC
AAATTGCCGCGCGGATGAATAACGAAGGGGCAATCCTTGCCAATGAGTTTTCCGCCAGTCGGGTAAAAGTGTTACATGCC
AATATCAGCCGCTGTGGCATCAGTAATGTTGCGCTCACACATTTTGATGGCCGCGTGTTTGGTGCGGCAGTGCCAGAAAT
GTTCGATGCCATTTTGCTGGACGCTCCCTGCTCTGGCGAAGGCGTGGTGCGTAAAGATCCCGATGCGCTAAAAAACTGGT
CACCAGAAAGCAATCAGGAAATCGCAGCTACACAACGGGAGCTTATCGACAGCGCCTTTCATGCATTACGTCCTGGTGGT
ACGCTGGTTTACTCGACCTGTACCTTAAACCAGGAAGAAAACGAAGCCGTTTGCCTGTGGCTGAAAGAGACTTACCCCGA
CGCAGTAGAGTTTTTACCACTTGGCGATCTCTTCCCTGGTGCAAACAAAGCGCTGACCGAAGAAGGCTTTTTGCATGTTT
TCCCACAAATTTACGACTGCGAAGGCTTCTTCGTTGCTCGTCTGCGTAAAACTCAGGCGATCCCCGCCTTACCCGCCCCC
AAATACAAAGTCGGTAATTTCCCGTTCAGCCCGGTGAAAGATCGCGAAGCCGGACAAATTCGTCAGGCGGCTGCAAGTGT
TGGCTTAAACTGGGATGGAAACCTGCGACTCTGGCAACGCGACAAAGAACTGTGGTTGTTCCCGGTGGGCATTGAAGCCC
TGATCGGTAAAGTCCGATTTTCTCGCTTGGGGATTAAACTTGCCGAAACGCACAACAAAGGTTATCGCTGGCAGCATGAA
GCAGTTATTGCCCTTGCCACCCCCGACAATGTGAACGCTTTTGAACTGACACCGCAGGAAGCGGAGGAGTGGTATCGCGG
GCGCGATGTTTACCCGCAAGCCGCGCCAGTGGCGGATGACGTGTTGGTTACTTTCCAGCATCAACCGATTGGTTTAGCCA
AACGGATTGGTTCGCGATTGAAAAACAGCTATCCGCGTGAACTGGTGCGCGATGGGAAACTTTTTACCGGTAACGCCTGA

Upstream 100 bases:

>100_bases
GGAACTGCGCTTCCCAAATAATGCCCACTGCTCCGGCGTGCCTGCGCCGGAGCGTTTATGCTAAACTGCGCGCCTGTTTT
TTTGCCAGTGGTACATGCTC

Downstream 100 bases:

>100_bases
CAGCGCACAAAAAAAGCGCACTTTTTGACTGGCACATTCGGCTGCCTCAACTAGGCTGAAAAATGGTGCGATCGGACTGG
TCGTACCACAACCGGCAGCT

Product: rRNA (cytosine-C(5)-)-methyltransferase RsmF

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDN
EDALPLGSTAEHLSGLFYIQEASSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGG
TLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAP
KYKVGNFPFSPVKDREAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHE
AVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA

Sequences:

>Translated_479_residues
MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDN
EDALPLGSTAEHLSGLFYIQEASSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGG
TLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAP
KYKVGNFPFSPVKDREAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHE
AVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA
>Mature_478_residues
AQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNE
DALPLGSTAEHLSGLFYIQEASSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHAN
ISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGGT
LVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPK
YKVGNFPFSPVKDREAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEA
VIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family

Homologues:

Organism=Homo sapiens, GI76150625, Length=303, Percent_Identity=36.6336633663366, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI76150623, Length=303, Percent_Identity=36.6336633663366, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI11545785, Length=166, Percent_Identity=37.9518072289157, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI39995082, Length=287, Percent_Identity=28.2229965156794, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI301336155, Length=286, Percent_Identity=28.3216783216783, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI32698918, Length=160, Percent_Identity=35, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI270288816, Length=239, Percent_Identity=31.7991631799163, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI23199998, Length=263, Percent_Identity=30.4182509505703, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI8922322, Length=228, Percent_Identity=31.5789473684211, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI270288818, Length=228, Percent_Identity=31.5789473684211, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI40316918, Length=209, Percent_Identity=29.1866028708134, Blast_Score=82, Evalue=2e-15,
Organism=Escherichia coli, GI87081985, Length=479, Percent_Identity=98.5386221294363, Blast_Score=978, Evalue=0.0,
Organism=Escherichia coli, GI2367212, Length=161, Percent_Identity=34.7826086956522, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=320, Percent_Identity=33.4375, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI25143471, Length=193, Percent_Identity=32.1243523316062, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71998419, Length=322, Percent_Identity=27.0186335403727, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115534021, Length=229, Percent_Identity=29.2576419213974, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=296, Percent_Identity=36.8243243243243, Blast_Score=167, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6319447, Length=206, Percent_Identity=32.0388349514563, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI22024126, Length=333, Percent_Identity=34.8348348348348, Blast_Score=152, Evalue=7e-37,
Organism=Drosophila melanogaster, GI21355201, Length=208, Percent_Identity=33.6538461538462, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24668781, Length=175, Percent_Identity=35.4285714285714, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI21356513, Length=175, Percent_Identity=35.4285714285714, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMF_ECO24 (A7ZMV8)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001463137.1
- ProteinModelPortal:   A7ZMV8
- SMR:   A7ZMV8
- STRING:   A7ZMV8
- EnsemblBacteria:   EBESCT00000021738
- GeneID:   5590679
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_2064
- eggNOG:   COG0144
- GeneTree:   EBGT00050000009912
- HOGENOM:   HBG726909
- ProtClustDB:   PRK11933
- BioCyc:   ECOL331111:ECE24377A_2064-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01579
- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023
- TIGRFAMs:   TIGR00446

Pfam domain/function: PF01189 Nol1_Nop2_Fmu

EC number: =2.1.1.178

Molecular weight: Translated: 53180; Mature: 53049

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: ACT_SITE 247-247 BINDING 149-149 BINDING 176-176 BINDING 194-194

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPY
CCCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEHHHHHHHHCCC
GWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADDNAPQR
CCEECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEEECCCCHHH
VMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHANISRCGISNVALTHFDGRVF
HHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEECCEEH
GAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGG
HHHHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
TLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDC
EEEEEEEECCCCCCCEEEEEEECCCCCHHHEEECCCCCCCCCCHHCCCCCEEECCEEECC
EGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKDREAGQIRQAAASVGLNWDGNLRLWQR
CCHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEE
DKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALATPDNVNAFELTPQE
CCEEEEEEECHHHHHHHHHHHHHCEEEEECCCCCCEEECCEEEEEECCCCCCEEEECHHH
AEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECCC
>Mature Secondary Structure 
AQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVNTLKISVADFLQLTAPY
CCCEEECCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCEEEEEEEEEHHHHHHHHCCC
GWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEASSMLPVAALFADDNAPQR
CCEECCCCCCCCCEEEECCCCCCCCCCCCHHHHHHEEEEECCCCCCCEEEEEECCCCHHH
VMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHANISRCGISNVALTHFDGRVF
HHHHHCCCCCCCEEEEEEECCCCCEEEECCCCCEEEEEECCHHHCCCCCEEEEEECCEEH
GAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESNQEIAATQRELIDSAFHALRPGG
HHHHHHHHHHHEECCCCCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
TLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGDLFPGANKALTEEGFLHVFPQIYDC
EEEEEEEECCCCCCCEEEEEEECCCCCHHHEEECCCCCCCCCCHHCCCCCEEECCEEECC
EGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKDREAGQIRQAAASVGLNWDGNLRLWQR
CCHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEE
DKELWLFPVGIEALIGKVRFSRLGIKLAETHNKGYRWQHEAVIALATPDNVNAFELTPQE
CCEEEEEEECHHHHHHHHHHHHHCEEEEECCCCCCEEECCEEEEEECCCCCCEEEECHHH
AEEWYRGRDVYPQAAPVADDVLVTFQHQPIGLAKRIGSRLKNSYPRELVRDGKLFTGNA
HHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA