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The map label for this gene is yoaE

Identifier: 218695378

GI number: 218695378

Start: 2050436

End: 2051992

Strand: Reverse

Name: yoaE

Synonym: EC55989_1989

Alternate gene names: 218695378

Gene position: 2051992-2050436 (Counterclockwise)

Preceding gene: 218695385

Following gene: 218695373

Centisome position: 39.81

GC content: 53.82

Gene sequence:

>1557_bases
ATGGAATTCTTAATGGACCCCTCAATTTGGGCGGGGCTACTCACGCTTGTTGTTCTCGAAATTGTGCTGGGTATCGATAA
CCTGGTCTTCATCGCCATTCTTGCTGACAAACTGCCGCCAAAACAACGCGATAAAGCGCGTTTGCTGGGGTTATCACTGG
CGCTGATTATGCGTCTGGGGCTGCTGTCGCTGATTTCATGGATGGTCACGCTGACCAAACCGCTATTTACCGTCATGGAT
TTCTCCTTCTCCGGACGCGACCTGATTATGTTGTTCGGGGGGATATTCTTGCTGTTCAAAGCAACAACCGAACTGCATGA
ACGGCTGGAAAACCGCGATCATGATTCCGGCCACGGTAAAGGCTACGCCAGTTTCTGGGTGGTCGTCACACAGATTGTCA
TCCTTGACGCCGTCTTCTCGTTGGATGCGGTAATTACTGCAGTAGGGATGGTTAACCATCTGCCGGTGATGATGGCGGCG
GTAGTGATTGCGATGGCGGTTATGTTGCTGGCATCCAAACCGCTGACGCGATTCGTTAACCAGCACCCCACGGTGGTGGT
GCTCTGTCTGAGCTTCCTGTTAATGATTGGTCTGAGTCTGGTGGCAGAAGGTTTCGGTTTCCACATTCCGAAAGGTTACC
TGTATGCCGCGATTGGCTTCTCGATCATCATCGAAGTGTTTAACCAGATTGCGCGTCGCAACTTTATTCGCCACCAGTCG
ACTTTGCCGCTGCGAGCGCGTACTGCCGATGCCATCCTGCGTTTGATGGGCGGGAAACGTCAGGCCAATGTTCAGCACGA
TGCCGATAACCCGATGCCGATGCCGATCCCGGAAGGTGCATTTGCCGAAGAAGAACGTTACATGATTAACGGCGTACTGA
CGCTGGCGTCGCGTTCTCTGCGCGGGATCATGACGCCGCGCGGTGAAATAAGCTGGGTTGACGCTAATCTCGGGGTCGAT
GAAATCCGCGAGCAACTGCTCTCTTCACCGCACAGTCTGTTCCCGGTATGTCGCGGTGAACTGGATGAAATCATTGGTAT
TGTACGTGCTAAAGAACTGCTGGTGGCGCTGGAAGAGGGCGTTGATGTGGCGGCGATTGCTTCGGCGTCTCCGGCGATTA
TCGTCCCGGAAACCCTCGATCCGATCAACCTGTTGGGCGTGCTGCGTCGTGCTCGCGGGAGCTTTGTTATCGTGACCAAC
GAGTTTGGTGTGGTACAAGGTCTGGTCACGCCGCTGGATGTGCTGGAAGCCATTGCGGGTGAATTCCCGGACGCTGACGA
AACGCCGGAAATCATTACTGATGGTGACGGCTGGCTGGTAAAAGGCGGTACAGATTTGCATGCCTTGCAGCAGGCGCTTG
ATGTTGAGCACCTTGCCGATGACGATGATATCGCGACGGTCGCGGGCCTCGTGATCTCGGCAAATGGTCACATTCCCCGT
GTGGGCGATGTGATTGATGTAGGGCCACTGCATATCACCATCATTGAAGCCAATGATTATCGTGTTGATCTGGTTCGCAT
TGTTAAAGAGCAACCGGCGCACGATGAAGATGAGTAA

Upstream 100 bases:

>100_bases
ACCTTTCTGGCTCTCTATGCCGCACCTTTCGTTTGCATTTTGTCGTTACGCCTGCATTATTTCTGGCGTCGAATAGCTAT
TCCTTAAGCAGGAGCTTGTC

Downstream 100 bases:

>100_bases
GCATTAACGTAACGGCATAATGGGCGTGATATGTCCATTATGCCGGGCGGGCGGCGGTTGGCTGCCCGCCAGCCATTTGG
GAAAATCACGTAGCGGCATC

Product: putative membrane protein fused with conserved domain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRDKARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMD
FSFSGRDLIMLFGGIFLLFKATTELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAIGFSIIIEVFNQIARRNFIRHQS
TLPLRARTADAILRLMGGKRQANVQHDADNPMPMPIPEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVD
EIREQLLSSPHSLFPVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGSFVIVTN
EFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQALDVEHLADDDDIATVAGLVISANGHIPR
VGDVIDVGPLHITIIEANDYRVDLVRIVKEQPAHDEDE

Sequences:

>Translated_518_residues
MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRDKARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMD
FSFSGRDLIMLFGGIFLLFKATTELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAIGFSIIIEVFNQIARRNFIRHQS
TLPLRARTADAILRLMGGKRQANVQHDADNPMPMPIPEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVD
EIREQLLSSPHSLFPVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGSFVIVTN
EFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQALDVEHLADDDDIATVAGLVISANGHIPR
VGDVIDVGPLHITIIEANDYRVDLVRIVKEQPAHDEDE
>Mature_518_residues
MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRDKARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMD
FSFSGRDLIMLFGGIFLLFKATTELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAIGFSIIIEVFNQIARRNFIRHQS
TLPLRARTADAILRLMGGKRQANVQHDADNPMPMPIPEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVD
EIREQLLSSPHSLFPVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGSFVIVTN
EFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQALDVEHLADDDDIATVAGLVISANGHIPR
VGDVIDVGPLHITIIEANDYRVDLVRIVKEQPAHDEDE

Specific function: Unknown

COG id: COG1253

COG function: function code R; Hemolysins and related proteins containing CBS domains

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 CBS domains

Homologues:

Organism=Homo sapiens, GI310128564, Length=213, Percent_Identity=26.7605633802817, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788119, Length=518, Percent_Identity=100, Blast_Score=1033, Evalue=0.0,
Organism=Escherichia coli, GI87082033, Length=520, Percent_Identity=50.1923076923077, Blast_Score=445, Evalue=1e-126,
Organism=Escherichia coli, GI1789197, Length=232, Percent_Identity=48.7068965517241, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1790664, Length=240, Percent_Identity=26.6666666666667, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1786879, Length=225, Percent_Identity=31.5555555555556, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI145693175, Length=235, Percent_Identity=29.7872340425532, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YOAE_ECO57 (P0AEC2)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E90944
- RefSeq:   NP_288252.1
- RefSeq:   NP_310552.1
- ProteinModelPortal:   P0AEC2
- EnsemblBacteria:   EBESCT00000025777
- EnsemblBacteria:   EBESCT00000057824
- GeneID:   914066
- GeneID:   961789
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z2859
- KEGG:   ecs:ECs2525
- GeneTree:   EBGT00050000010862
- HOGENOM:   HBG470183
- OMA:   QIARHNF
- ProtClustDB:   CLSK866497
- BioCyc:   ECOL83334:ECS2525-MONOMER
- InterPro:   IPR016169
- InterPro:   IPR000644
- InterPro:   IPR005496
- InterPro:   IPR005170
- Gene3D:   G3DSA:3.30.465.10

Pfam domain/function: PF00571 CBS; PF03471 CorC_HlyC; PF03741 TerC

EC number: NA

Molecular weight: Translated: 56529; Mature: 56529

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS51371 CBS

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x19bb6070)-; HASH(0x1a3b132c)-; HASH(0x1a0a7dd4)-; HASH(0x1a0de9f4)-; HASH(0x95004c8)-; HASH(0x19d53f80)-; HASH(0x1a0bc6f8)-; HASH(0x1a2107a4)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCEEECCCEEEEEEECCCCHHHHHHHHHHCCCCCCCH
>Mature Secondary Structure
MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRDKARLLGLSLALIMRLG
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKATTELHERLENRDHDSGHGK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAAVVIAMAVMLLASKPLTRFVN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHC
QHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAIGFSIIIEVFNQIARRNFIRHQS
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TLPLRARTADAILRLMGGKRQANVQHDADNPMPMPIPEGAFAEEERYMINGVLTLASRSL
CCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPVCRGELDEIIGIVRAKELLVALEEG
HHCCCCCCCEEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
VDVAAIASASPAIIVPETLDPINLLGVLRRARGSFVIVTNEFGVVQGLVTPLDVLEAIAG
CCEEEECCCCCCEEECCCCCHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHC
EFPDADETPEIITDGDGWLVKGGTDLHALQQALDVEHLADDDDIATVAGLVISANGHIPR
CCCCCCCCCCEEECCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHEEEECCCCCCC
VGDVIDVGPLHITIIEANDYRVDLVRIVKEQPAHDEDE
CCCEEECCCEEEEEEECCCCHHHHHHHHHHCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796