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The map label for this gene is cmr

Identifier: 218694316

GI number: 218694316

Start: 945048

End: 946280

Strand: Direct

Name: cmr

Synonym: EC55989_0887

Alternate gene names: 218694316

Gene position: 945048-946280 (Clockwise)

Preceding gene: 218694313

Following gene: 218694320

Centisome position: 18.33

GC content: 52.15

Gene sequence:

>1233_bases
ATGCAAAATAAATTAGCTTCCGGTGCCAGGCTTGGACGTCAGGCGTTACTTTTCCCTCTCTGTCTGGTGCTTTACGAATT
TTCAACCTATATCGGCAACGATATGATTCAACCCGGTATGTTGGCCGTGGTGGAACAATATCAGGCGGGCATTGATTGGG
TTCCTACTTCAATGACCGCATATCTGGCGGGCGGGATGTTTTTACAATGGCTCCTGGGGCCGCTGTCGGATCGTATTGGT
CGCCGTCCGGTGATGCTGGCGGGGGTGGTGTGGTTTATCATCACCTGTCTGGCAATATTACTGGCGCAAAACATTGAACA
ATTCACTCTGTTGCGCTTCTTGCAGGGCATAAGCCTCTGTTTCATTGGCGCTGTGGGATACGCCGCAATTCAGGAATCCT
TCGAAGAGGCGGTTTGTATCAAGATCACCGCGCTGATGGCGAACGTGGCGCTGATTGCTCCGCTACTTGGTCCGCTGGTG
GGCGCGGCGTGGATCCATGTGCTGCCCTGGGAAGGGATGTTTGTCTTGTTTGCCGCATTGGCAGCGATCTCCTTTTTCGG
TCTGCAACAAGCCATGCCTGAAACCGCCACGCGTATAGGCGAGAAACTGTCACTGAAAGAACTCGGTCGTGACTATAAGC
TGGTGCTGAAGAACGGCCGCTTTGTGGCGGGGGCGCTGGCGCTGGGATTCGTTAGCCTGCCATTGCTGGCGTGGATCGCC
CAGTCGCCGATTATCATCATTACCGGCGAGCAGTTGAGCAGCTATGAATATGGCTTGCTGCAAGTGCCTATTTTCGGGGC
GTTAATTGCGGGTAACTTGCTGTTAGCGCGTCTGACCTCGCGCCGCACCGTACGTTCGCTGATTATTATGGGCGGCTGGC
CGATTATTATTGGTCTGTTGGTCGCTGCTGCGGCAACGGTTATCTCATCGCACGCGTATTTATGGATGACCGCCGGGTTA
AGTATTTATGCTTTCGGCATTGGTCTGGCGAATGCGGGACTGGTGCGATTAACCCTGTTTGCCAGCGATATGAGTAAAGG
TACGGTTTCTGCGGCGATGGGAATGCTGCAAATGCTGATCTTTACCGTTGGTATTGAAATCAGCAAACATGCCTGGCTGA
ACGGGGGCAACGGACAGTTTAATCTCTTCAACCTTGTCAACGGAATTTTGTGGCTGTCGCTGATGGTTATCTTTTTAAAA
GATAAACAGATGGGAAATTCTCACGAAGGGTAA

Upstream 100 bases:

>100_bases
GTAAGAATGTGCTTAACCGTGGTTTCAGCTACAAAATTCGCTTTCTCGTTAGCTGTGCTTTTATTAAACTCTGCGCGATT
ATTATTGGCGAAGAAATTGC

Downstream 100 bases:

>100_bases
AAAAATGCCTGATTGCTTTGTGCGATCAGGCATTCTCGAATTAATGGTGATGGTCGTCAATCTGGTGTTCGATAACCATC
CCTTCACCTACGCTGGCAAG

Product: multidrug efflux system protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Chloramphenicol resistance pump cmr [H]

Number of amino acids: Translated: 410; Mature: 410

Protein sequence:

>410_residues
MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLAGVVWFIITCLAILLAQNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIA
QSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIIIGLLVAAAATVISSHAYLWMTAGL
SIYAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWLSLMVIFLK
DKQMGNSHEG

Sequences:

>Translated_410_residues
MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLAGVVWFIITCLAILLAQNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIA
QSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIIIGLLVAAAATVISSHAYLWMTAGL
SIYAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWLSLMVIFLK
DKQMGNSHEG
>Mature_410_residues
MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIG
RRPVMLAGVVWFIITCLAILLAQNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIA
QSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIIIGLLVAAAATVISSHAYLWMTAGL
SIYAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWLSLMVIFLK
DKQMGNSHEG

Specific function: Efflux pump driven by the proton motive force. Confers resistance to basic, aromatic and lipophilic drugs as well as neutral, non-aromatic, zwitterionic or non-aromatic hydrophilic antibiotics [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787065, Length=410, Percent_Identity=99.0243902439024, Blast_Score=806, Evalue=0.0,
Organism=Escherichia coli, GI1790794, Length=376, Percent_Identity=41.4893617021277, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI48994889, Length=217, Percent_Identity=25.8064516129032, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788509, Length=303, Percent_Identity=23.4323432343234, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1790146, Length=168, Percent_Identity=27.9761904761905, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44305; Mature: 44305

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGIDWVPTSMTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
YLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLAQNIEQFTLLRFLQGISLC
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
FIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVLPWEGMFVLFAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIA
HHHHHHHHHHHCHHHHHHHHCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
QSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIIIGLL
CCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMLQMLI
HHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCHHEEEEEHHHCCCHHHHHHHHHHHHHH
FTVGIEISKHAWLNGGNGQFNLFNLVNGILWLSLMVIFLKDKQMGNSHEG
HHHCCCEECCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVVEQYQAGIDWVPTSMTA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCHHHHH
YLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLAQNIEQFTLLRFLQGISLC
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
FIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLVGAAWIHVLPWEGMFVLFAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
AAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKNGRFVAGALALGFVSLPLLAWIA
HHHHHHHHHHHCHHHHHHHHCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHH
QSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARLTSRRTVRSLIIMGGWPIIIGLL
CCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVRLTLFASDMSKGTVSAAMGMLQMLI
HHHHHHHHHCCCCEEEECCCHHHHHHHHHHCCCHHEEEEEHHHCCCHHHHHHHHHHHHHH
FTVGIEISKHAWLNGGNGQFNLFNLVNGILWLSLMVIFLKDKQMGNSHEG
HHHCCCEECCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]