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The map label for this gene is cof

Identifier: 218693909

GI number: 218693909

Start: 494724

End: 495542

Strand: Direct

Name: cof

Synonym: EC55989_0460

Alternate gene names: 218693909

Gene position: 494724-495542 (Clockwise)

Preceding gene: 218693906

Following gene: 218693910

Centisome position: 9.6

GC content: 51.4

Gene sequence:

>819_bases
ATGGCTCGTCTGGCAGCATTTGATATGGATGGCACTTTATTGATGCCTGACCATCATTTAGGTGAGAAAACCCTCTCTAC
TTTGGCGCGACTGCGTGAACGCGACATTACCCTCACTTTTGCCACGGGGCGTCATGCGCTGGAGATGCAGCATATTCTCG
GGGCGCTATCGCTGGATGCATATTTGATTACCGGCAACGGAACGCGCGTGCATTCTCTGGAAGGTGAACTTTTACATCGT
GATGATTTACCTGCGGATGTCGCGGAGCTGGTGCTGTATCAGCAATGGGATACCCGAGCCAGCATGCATATCTTCAATGA
CGACGGTTGGTTTACCGGGAAAGAGATCCCTGCGTTGTTGCAGGCATTTGTCTATAGCGGTTTTCGTTATCAGATAATCG
ATGTCAAAAAAATGCCACTCGGCAGAGTCACCAAGATCTGCTTCTGTGGCGGTCACGACGATCTTACACGCTTGCAGATC
CAGCTATACGAAGCATTAGGCGAGCGTGCACATTTGTGTTTTTCCGCCACGGATTGCCTCGAAGTGCTGCCGGTGGGCTG
CAATAAAGGCGCTGCATTGACGGTGCTGACCCAACATTTAGGTTTATCGTTGCGCGATTGCATGGCCTTTGGTGATGCGA
TGAACGATCGCGAAATGTTAGGCAGCGTCGGTAGCGGGTTTATTATGGGCAATGCGATGCCGCAACTGCGCGCGGAGCTC
CCGCATTTACCGGTGATTGGACATTGCCGAAATCAGGCTGTCTCTCACTATTTGACGCACTGGCTGGACTATCCACATCT
ACCTTATTCCCCCGAATAA

Upstream 100 bases:

>100_bases
TATAAACCCGGAACAATATTATTTAACTATTCACTATTACTTCCGTATATATCAGGTGATACTCAATCACCATTAACCGT
GTCACAGAGTGGAGAAAGAA

Downstream 100 bases:

>100_bases
CGAGATCCCTTCCAGCACCGGGCAATTGCCCGGTTTTTTTTGCGTTGAATTTGTCATTTTGTGCCGTGGTGTTTAAACCG
CACAGAATAAATTGTCGTGA

Product: thiamin pyrimidine pyrophosphate hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 271

Protein sequence:

>272_residues
MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDAYLITGNGTRVHSLEGELLHR
DDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAEL
PHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE

Sequences:

>Translated_272_residues
MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDAYLITGNGTRVHSLEGELLHR
DDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQI
QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAEL
PHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE
>Mature_271_residues
ARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRD
DLPADVAELVLYQQWDTRASMHIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQIQ
LYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAELP
HLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE

Specific function: Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081741, Length=272, Percent_Identity=98.8970588235294, Blast_Score=554, Evalue=1e-159,
Organism=Escherichia coli, GI48994981, Length=260, Percent_Identity=38.8461538461538, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI2367265, Length=243, Percent_Identity=26.3374485596708, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: NA

Molecular weight: Translated: 30340; Mature: 30209

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS01228 COF_1 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDA
CCCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCE
YLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKEIPALL
EEEECCCCEEEECCCCEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEECCHHHHHHH
QAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQIQLYEALGERAHLCFSATDCL
HHHHHCCCEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHEEEECHHHHH
EVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAEL
HHEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHCCCCCEEECCCCHHHHHCC
PHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE
CCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGRHALEMQHILGALSLDA
CCEEEECCCCEEECCCCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCE
YLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASMHIFNDDGWFTGKEIPALL
EEEECCCCEEEECCCCEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCCEECCHHHHHHH
QAFVYSGFRYQIIDVKKMPLGRVTKICFCGGHDDLTRLQIQLYEALGERAHLCFSATDCL
HHHHHCCCEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCHHEEEECHHHHH
EVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDREMLGSVGSGFIMGNAMPQLRAEL
HHEECCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHCCCCCEEECCCCHHHHHCC
PHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE
CCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA