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The map label for this gene is aroL

Identifier: 218693849

GI number: 218693849

Start: 431621

End: 432145

Strand: Direct

Name: aroL

Synonym: EC55989_0397

Alternate gene names: 218693849

Gene position: 431621-432145 (Clockwise)

Preceding gene: 218693848

Following gene: 218693850

Centisome position: 8.37

GC content: 51.81

Gene sequence:

>525_bases
ATGACACAACCTCTTTTTCTGATCGGGCCTCGGGGCTGTGGTAAAACAACGGTCGGAATGGCCCTTGCCGATTCGCTTAA
CCGTCGGTTTGTCGATACCGATCAGTGGTTGCAATCACAGCTCAATATGACGGTCGCGGAGATCGTCGAAAGGGAAGAGT
GGGCGGGATTTCGCGCCAGAGAAACGGCGGCGCTGGAAGCGGTAACTGCGCCATCCACCGTTATCGCTACAGGCGGCGGC
ATTATTCTGACGGAATTTAATCGTCACTTTATGCAAAATAACGGGATCGTGGTTTATTTGTGTGCGCCAGTATCAGTCCT
GGTTAACCGACTGCAAGCTGCACCGGAAGAAGATTTACGGCCAACCTTAACGGGAAAACCGCTGAGCGAAGAAGTTCAGG
AAGTGCTGGAAGAACGCGATGCGCTATATCGCGAAGTTGCGCATATTATCATCGACGCAACAAACGAACCCAGCCAGGTG
ATTTCTGAAATTCGTAGCGCCCTGGCACAGACGATCAATTGTTGA

Upstream 100 bases:

>100_bases
TTTTTTCTTTACAATCGAAATTGTACTAGTTTGATGGTATGATCGCTATTCTCATGACACCGGCTTTCGCCGCATTGCGA
CCTATTGGGGAAAACCCACG

Downstream 100 bases:

>100_bases
TTTTAGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAG
CATATATAGTGACGATTGAA

Product: shikimate kinase II

Products: NA

Alternate protein names: SK 2

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWAGFRARETAALEAVTAPSTVIATGGG
IILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
ISEIRSALAQTINC

Sequences:

>Translated_174_residues
MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWAGFRARETAALEAVTAPSTVIATGGG
IILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
ISEIRSALAQTINC
>Mature_173_residues
TQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGI
ILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQVI
SEIRSALAQTINC

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family. AroL subfamily

Homologues:

Organism=Escherichia coli, GI1786587, Length=174, Percent_Identity=100, Blast_Score=356, Evalue=1e-100,
Organism=Escherichia coli, GI87082255, Length=160, Percent_Identity=33.75, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROL_ECO24 (A7ZID7)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001461566.1
- ProteinModelPortal:   A7ZID7
- SMR:   A7ZID7
- STRING:   A7ZID7
- EnsemblBacteria:   EBESCT00000021803
- GeneID:   5589344
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_0415
- eggNOG:   COG0703
- GeneTree:   EBGT00050000009881
- HOGENOM:   HBG335815
- OMA:   VEKEGWE
- ProtClustDB:   PRK03731
- BioCyc:   ECOL331111:ECE24377A_0415-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01269
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 19151; Mature: 19020

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 34-34 BINDING 58-58 BINDING 79-79 BINDING 120-120 BINDING 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWAGFRAR
CCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCHH
ETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLR
HHHHHHHHCCCCEEEECCCCEEEEHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCC
PTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQVISEIRSALAQTINC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMTVAEIVEREEWAGFRAR
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHHHHHHHHHCCCCHH
ETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCAPVSVLVNRLQAAPEEDLR
HHHHHHHHCCCCEEEECCCCEEEEHHHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCCC
PTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQVISEIRSALAQTINC
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA