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The map label for this gene is cynT

Identifier: 218693803

GI number: 218693803

Start: 384978

End: 385637

Strand: Direct

Name: cynT

Synonym: EC55989_0346

Alternate gene names: 218693803

Gene position: 384978-385637 (Clockwise)

Preceding gene: 218693801

Following gene: 218693804

Centisome position: 7.47

GC content: 57.27

Gene sequence:

>660_bases
GTGAAAGAGATTATTGATGGATTCCTTAAATTCCAGCGCGAGGCATTTCCGAAGCGGGAAGCCTTGTTTAAACAGCTGGC
GACACAGCAAAGCCCGCGCACACTTTTTATCTCCTGCTCCGACAGCCGTCTGGTCCCTGAGCTGGTGACGCAACGTGAGC
CTGGCGATCTGTTCGTTATTCGCAACGCGGGCAATATCGTCCCTTCCTACGGGCCGGAACCCGGTGGCGTTTCTGCTTCG
GTGGAGTATGCCGTCGCTGCGCTTCGGGTATCTGACATTGTGATTTGTGGTCATTCCAACTGTGGCGCGATGACCGCCAT
TGCCAGCTGTCAGTGCATGGACCATATGCCTGCCGTCTCCCACTGGCTGCGTTATGCCGATTCAGCCCGCGTCGTTAATG
AGGCGCGCCCGCATTCCGATTTACCGTCAAAAGCTGCGGCGATGGTACGTGAAAACGTCATTGCTCAGTTGGCTAATTTG
CAAACTCATCCATCGGTGCGCCTGGCGCTCGAAGAGGGGCGGATCGCCCTGCACGGCTGGGTCTACGACATTGAAAGCGG
CAGCATCGCAGCTTTTGACGGCGCAACCCGCCAGTTTGTGCCACTGGCCGCTAATCCTCGCGTTTGTGCCATACCGCTAC
GCCAACCGACCGCAGCGTAA

Upstream 100 bases:

>100_bases
TATAAGTAAATCCAATAGAACTCATCATAAATGAGACTTTTACCTTATGACAATCGGCGAGTAGTCTGCCTCTCATTCCA
GAGACAGACAGAGGTTAACG

Downstream 100 bases:

>100_bases
CCTTATTTTTAAACCATCAGGAGATCCACCATGATTCAGTCACAAATTAACCGCAATATTCGTCTTGATCTTGCCGATGC
CATTTTGCTCAGCAAAGCTA

Product: carbonic anhydrase

Products: NA

Alternate protein names: Carbonate dehydratase 1

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRLVPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSAS
VEYAVAALRVSDIVICGHSNCGAMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTAA

Sequences:

>Translated_219_residues
MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRLVPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSAS
VEYAVAALRVSDIVICGHSNCGAMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTAA
>Mature_219_residues
MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRLVPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSAS
VEYAVAALRVSDIVICGHSNCGAMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTAA

Specific function: Reversible hydration of carbon dioxide. Carbon dioxide formed in the bicarbonate-dependent decomposition of cyanate by cyanase (CynS) diffuses out of the cell faster than it would be hydrated to bicarbonate, so the apparent function of this enzyme is to c

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family

Homologues:

Organism=Escherichia coli, GI1786534, Length=219, Percent_Identity=100, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1786318, Length=180, Percent_Identity=32.7777777777778, Blast_Score=89, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI25147564, Length=237, Percent_Identity=29.1139240506329, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI115532990, Length=237, Percent_Identity=26.5822784810127, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324292, Length=178, Percent_Identity=29.7752808988764, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24645213, Length=245, Percent_Identity=23.6734693877551, Blast_Score=83, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYNT_ECO57 (P0ABF0)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85528
- PIR:   H90677
- RefSeq:   NP_286080.1
- RefSeq:   NP_308419.1
- ProteinModelPortal:   P0ABF0
- SMR:   P0ABF0
- EnsemblBacteria:   EBESCT00000027255
- EnsemblBacteria:   EBESCT00000058004
- GeneID:   914494
- GeneID:   957265
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0435
- KEGG:   ecs:ECs0392
- GeneTree:   EBGT00050000009995
- HOGENOM:   HBG711150
- OMA:   LRSYPCV
- ProtClustDB:   CLSK866979
- BioCyc:   ECOL83334:ECS0392-MONOMER
- InterPro:   IPR001765
- InterPro:   IPR015892
- Gene3D:   G3DSA:3.40.1050.10
- PANTHER:   PTHR11002
- SMART:   SM00947

Pfam domain/function: PF00484 Pro_CA; SSF53056 Prok_plnt_COanhd

EC number: =4.2.1.1

Molecular weight: Translated: 23764; Mature: 23764

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1; PS00705 PROK_CO2_ANHYDRASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRLVPELVTQREPGDLFVI
CHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCCEEEE
RNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCGAMTAIASCQCMDHMPAVS
ECCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHH
HWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANLQTHPSVRLALEEGRIALHGW
HHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEE
VYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTAA
EEEECCCCEEEECCCCCEEEEECCCCCEEEEECCCCCCC
>Mature Secondary Structure
MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRLVPELVTQREPGDLFVI
CHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCCEEEE
RNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCGAMTAIASCQCMDHMPAVS
ECCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEEECCCCCCHHHHHHHHHHHHCCHHHH
HWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANLQTHPSVRLALEEGRIALHGW
HHHHHCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEE
VYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTAA
EEEECCCCEEEECCCCCEEEEECCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796