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The map label for this gene is prpC

Identifier: 218693796

GI number: 218693796

Start: 375112

End: 376281

Strand: Direct

Name: prpC

Synonym: EC55989_0339

Alternate gene names: 218693796

Gene position: 375112-376281 (Clockwise)

Preceding gene: 218693795

Following gene: 218693797

Centisome position: 7.28

GC content: 55.38

Gene sequence:

>1170_bases
ATGAGCGACACAACGATCCTGCAAAACAGTACCCATGTCATTAAACCGAAAAAATCGGTAGCACTTTCTGGCGTTCCGGC
GGGCAATACGGCGCTCTGCACCGTGGGTAAAAGTGGCAATGACCTGCATTACCGTGGCTACGATATTCTTGATCTGGCGG
AACATTGCGAATTTGAAGAAGTGGCGCACCTGCTGATCCACGGCAAACTGCCGACCCGTGACGAACTCGCCGCTTACAAA
ACGAAACTGAAAGCCCTGCGTGGTTTACCAGCTAATGTGCGTACCGTGCTGGAAGCCTTACCGGCGGCGTCGCACCCGAT
GGATGTCATGCGCACCGGCGTTTCCGCGCTCGGCTGCACACTGCCAGAAAAAGAGGGGCACACTGTCTCTGGCGCGCGGG
ATATTGCCGACAAACTGCTGGCATCACTTAGCTCGATTCTCCTTTATTGGTATCACTACAGTCACAACGGCGAACGCATC
CAGCCGGAAACCGATGACGACTCCATCGGCGGTCACTTCCTGCATCTGCTGCACGGCGAAAAGCCATCGCAAAGCTGGGA
AAAGGCGATGCATATCTCGCTGGTGCTGTACGCCGAACACGAGTTTAACGCCTCCACCTTTACCAGTCGGGTGATTGCGG
GCACCGGCTCTGATATGTATTCCGCGATTATTGGCGCGATTGGCGCACTGCGCGGGCCAAAACACGGCGGGGCGAATGAA
GTGTCGCTGGAGATCCAGCAACGCTACGAAACGCCGGACGAAGCCGAAGCAGATATCCGCAAGCGCGTGGAAAACAAAGA
AGTGGTCATTGGTTTTGGTCATCCGGTTTACACCATCGCTGACCCGCGCCACCAGGTGATTAAACGTGTGGCGAAGCAGC
TCTCGCAGGAAGGCGGCTCGCTGAAGATGTACAACATCGCCGATCGCCTGGAAACGGTGATGTGGGAGAGCAAAAAGATG
TTCCCCAATCTCGACTGGTTCTCTGCTGTTTCCTACAACATGATGGGCGTTCCCACCGAGATGTTCACACCACTGTTTGT
TATCGCCCGCGTCACCGGCTGGGCGGCGCACATTATCGAACAACGTCAGGACAACAAAATTATCCGTCCTTCCGCCAATT
ATGTTGGACCGGAAGACCGCCCGTTTGTCGCGCTGGATAAGCGCCAGTAA

Upstream 100 bases:

>100_bases
ACTACCAGTACGAAGCGAAGCTCGACGACCTGTTTGCCCGTAGCCAGGTGAAATAAAACACAACGAAAAATATCTCGACC
CTACAAATGACGAGGACAAT

Downstream 100 bases:

>100_bases
ACCTCTACGAATAACAATAAGGAAACGTACCCAATGTCAGCTCAAATCAACAACATCCGCCCGGAATTTGATCGTGAAAT
CGTTGATATCGTCGATTACG

Product: methylcitrate synthase

Products: NA

Alternate protein names: Citrate synthase 2; Methylcitrate synthase [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHYRGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYK
TKLKALRGLPANVRTVLEALPAASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSDMYSAIIGAIGALRGPKHGGANE
VSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPVYTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKM
FPNLDWFSAVSYNMMGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ

Sequences:

>Translated_389_residues
MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHYRGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYK
TKLKALRGLPANVRTVLEALPAASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSDMYSAIIGAIGALRGPKHGGANE
VSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPVYTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKM
FPNLDWFSAVSYNMMGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ
>Mature_388_residues
SDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHYRGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKT
KLKALRGLPANVRTVLEALPAASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERIQ
PETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSDMYSAIIGAIGALRGPKHGGANEV
SLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPVYTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMF
PNLDWFSAVSYNMMGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ

Specific function: Catalyzes the synthesis of 2-methylcitrate from propionyl-CoA and oxaloacetate. Also catalyzes the condensation of oxaloacetate with acetyl-CoA but with a lower specificity [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786527, Length=389, Percent_Identity=99.7429305912596, Blast_Score=809, Evalue=0.0,
Organism=Escherichia coli, GI1786939, Length=371, Percent_Identity=31.5363881401617, Blast_Score=171, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17555174, Length=358, Percent_Identity=25.4189944134078, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319850, Length=376, Percent_Identity=23.936170212766, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324328, Length=331, Percent_Identity=25.6797583081571, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640124, Length=330, Percent_Identity=24.2424242424242, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24640126, Length=330, Percent_Identity=24.2424242424242, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.5 [H]

Molecular weight: Translated: 43104; Mature: 42972

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHYRGYDILDLAEHCEFEE
CCCCEEEECCCEEECCCCCEEECCCCCCCCEEEEECCCCCCCEECCCHHHHHHHHCCHHH
VAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPAASHPMDVMRTGVSALGCT
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
LPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERIQPETDDDSIGGHFLHLLHGE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHEEEEECCC
KPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSDMYSAIIGAIGALRGPKHGGANE
CCCHHHHHHEEEEEEEEEECCCCCHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCCCCC
VSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPVYTIADPRHQVIKRVAKQLSQEGGS
EEEEHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHCCCC
LKMYNIADRLETVMWESKKMFPNLDWFSAVSYNMMGVPTEMFTPLFVIARVTGWAAHIIE
EEEEEHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QRQDNKIIRPSANYVGPEDRPFVALDKRQ
HHCCCCEECCCCCCCCCCCCCEEEECCCC
>Mature Secondary Structure 
SDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHYRGYDILDLAEHCEFEE
CCCEEEECCCEEECCCCCEEECCCCCCCCEEEEECCCCCCCEECCCHHHHHHHHCCHHH
VAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPAASHPMDVMRTGVSALGCT
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCC
LPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERIQPETDDDSIGGHFLHLLHGE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCHHEEEEECCC
KPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSDMYSAIIGAIGALRGPKHGGANE
CCCHHHHHHEEEEEEEEEECCCCCHHHHHHHEECCCCHHHHHHHHHHHHCCCCCCCCCCC
VSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPVYTIADPRHQVIKRVAKQLSQEGGS
EEEEHHHHCCCCCHHHHHHHHHCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHCCCC
LKMYNIADRLETVMWESKKMFPNLDWFSAVSYNMMGVPTEMFTPLFVIARVTGWAAHIIE
EEEEEHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QRQDNKIIRPSANYVGPEDRPFVALDKRQ
HHCCCCEECCCCCCCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 8508809; 9325432 [H]