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The map label for this gene is proB

Identifier: 218693725

GI number: 218693725

Start: 293744

End: 294847

Strand: Direct

Name: proB

Synonym: EC55989_0266

Alternate gene names: 218693725

Gene position: 293744-294847 (Clockwise)

Preceding gene: 218693723

Following gene: 218693726

Centisome position: 5.7

GC content: 55.98

Gene sequence:

>1104_bases
ATGAGTGACAGCCAGACGCTGGTGGTAAAACTCGGCACCAGTGTGCTAACAGGCGGATCGCGCCGTCTGAACCGTGCCCA
TATCGTTGAACTTGTTCGCCAGTGCGCGCAGTTACATGCCGCCGGGCATCGGATTGTTATTGTGACGTCGGGCGCGATCG
CCGCCGGACGTGAGCACCTGGGTTACCCGGAACTGCCAGCGACTATCGCCTCGAAACAACTGCTGGCAGCGGTAGGGCAG
AGTCGACTGATTCAACTGTGGGAACAGCTGTTTTCGATTTATGGCATTCACGTCGGGCAAATGCTGCTGACCCGTGCTGA
TATGGAAGACCGTGAACGCTTCCTGAACGCCCGCGACACCCTGCGTGCGTTGCTCGATAACAATATCGTTCCGGTAATCA
ATGAGAACGATGCTGTCGCTACGGCAGAGATTAAAGTCGGCGATAACGATAACCTTTCTGCGCTGGCGGCGATTCTGGCG
GGTGCCGATAAACTGTTGCTGCTGACCGATCAAAAAGGTTTGTATACCGCTGACCCGCGCAGCAATCCGCAGGCAGAACT
GATTAAAGATGTTTACGGCATTGATGACGCACTGCGCGCGATTGCCGGTGACAGCGTTTCAGGCCTCGGAACTGGCGGCA
TGAGTACCAAATTGCAGGCCGCTGACGTGGCTTGCCGTGCGGGTATCGACACCATTATTGCCGCGGGCAGCAAGCCGGGC
GTTATTGGTGATGTGATGGAAGGCATTTCCGTCGGTACGCTGTTCCATGCCCAGGCGACTCCGCTTGAAAACCGTAAACG
CTGGATTTTCGGTGCGCCGCCGGCGGGTGAAATCACGGTAGATGAAGGGGCAACTGCCGCCATTCTTGAACGCGGCAGCT
CCCTGTTGCCGAAAGGCATTAAAAGCGTGACTGGCAATTTCTCGCGTGGTGAAGTCATCCGCATTTGCAACCTCGAAGGT
CGCGATATCGCCCACGGCGTCAGTCGTTACAACAGCGATGCATTACGCCGTATTGCCGGGCACCACTCGCAAGAAATTGA
TGCAATACTGGGATATGAATACGGCCCGGTTGCCGTTCACCGTGATGACATGATTACCCGTTAA

Upstream 100 bases:

>100_bases
AAAACGCCATGCTTTGCTTGCAGATGGTTGGCAACCGACGACAGTCCTGCTAAAACGTTCGTTTGATATCATTTTTCCTA
AAATTGAATGGCAGAGAATC

Downstream 100 bases:

>100_bases
GGAGCAGGCTGATGCTGGAACAAATGGGCATTGCCGCGAAGCAAGCCTCGTATAAATTAGCGCAACTCTCCAGCCGCGAA
AAAAATCGCGTGCTGGAAAA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQ
SRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPG
VIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAGEITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEG
RDIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR

Sequences:

>Translated_367_residues
MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQ
SRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPG
VIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAGEITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEG
RDIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR
>Mature_366_residues
SDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQS
RLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILAG
ADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPGV
IGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAGEITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGR
DIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=288, Percent_Identity=34.0277777777778, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI21361368, Length=290, Percent_Identity=33.7931034482759, Blast_Score=134, Evalue=1e-31,
Organism=Escherichia coli, GI1786437, Length=367, Percent_Identity=100, Blast_Score=739, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17569969, Length=272, Percent_Identity=34.1911764705882, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17569967, Length=272, Percent_Identity=34.1911764705882, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321822, Length=418, Percent_Identity=35.6459330143541, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6320506, Length=417, Percent_Identity=34.2925659472422, Blast_Score=216, Evalue=6e-57,
Organism=Drosophila melanogaster, GI21357643, Length=298, Percent_Identity=33.8926174496644, Blast_Score=135, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39057; Mature: 38926

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHL
CCCCCEEEEEECCHHHCCCCHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHC
GYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQKGLYTADPR
HHHHHCCCCEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCEECCCC
SNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPG
CCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHCCCHHEEECCCCCC
VIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAGEITVDEGATAAILERGSSLLPKGI
CHHHHHHCCHHCEEEECCCCCCCCCCCEEEECCCCCCEEECCCCHHHHHHCCCHHHHHHH
KSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVH
HHHCCCCCCCCEEEEECCCCCHHHHHHHHHCHHHHHHHHCCCCHHHHHHHCCCCCCEEEE
RDDMITR
CCCCCCC
>Mature Secondary Structure 
SDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHRIVIVTSGAIAAGREHL
CCCCEEEEEECCHHHCCCCHHCCHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHHC
GYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADMEDRERFLNARDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
LRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQKGLYTADPR
HHHHHCCCCEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCEECCCC
SNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPG
CCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHCCCHHEEECCCCCC
VIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAGEITVDEGATAAILERGSSLLPKGI
CHHHHHHCCHHCEEEECCCCCCCCCCCEEEECCCCCCEEECCCCHHHHHHCCCHHHHHHH
KSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVH
HHHCCCCCCCCEEEEECCCCCHHHHHHHHHCHHHHHHHHCCCCHHHHHHHCCCCCCEEEE
RDDMITR
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA