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The map label for this gene is yadI

Identifier: 218693595

GI number: 218693595

Start: 144630

End: 145070

Strand: Direct

Name: yadI

Synonym: EC55989_0123

Alternate gene names: 218693595

Gene position: 144630-145070 (Clockwise)

Preceding gene: 218693594

Following gene: 218693596

Centisome position: 2.81

GC content: 49.66

Gene sequence:

>441_bases
ATGTTAGGTTGGGTAATTACCTGTCACGATGACCGGGCGCAAGAGATACTGGATGCGCTGGAGAAAAAACATGGGGCGCT
TCTTCAGTGCCGGGCCGTGAATTTCTGGCGTGGGTTAAGCTCTAATATGCTCAGCCGCATGATGTGCGATGCTCTGCATG
AAGCGGACTCTGGTGAGGGTGTCATCTTCTTAACCGATATAGCCGGAGCGCCACCGTACCGCGTGGCTTCATTATTAAGC
CACAAACACTCCCGTTGCGAAGTGATTTCTGGTGTCACGTTACCGTTAATTGAACAGATGATGGCTTGCCGTGAAACCAT
GACCAGTTCAGAGTTTCGCGAGCGTATTGTCGAACTGGGTGGGCCGGAGGTGAGTAGTCTTTGGCACCAACAACAAAAAA
ATCCGCCTTTCGTCCTCAAACATAATTTGTATGAGTATTAA

Upstream 100 bases:

>100_bases
ATTTCCTCTCCTCTGGATTTGGGGGAGAGGAGTTTTGACGGCTATCACCCTTTATCAACAATGGTCAGGGTAGACTGATT
TTCGGCTAAGGAGGAAGGCG

Downstream 100 bases:

>100_bases
CCCACGATTCTTATAGCGCTTTTGCTACAATAAAAGCGTTGTTTCACCCTCGGTTATTTTTTCATGTACAAACAAGCTGT
TATTCTCCTGCTGATGCTGT

Product: putative PTS Enzyme IIA

Products: protein histidine; sugar phosphate

Alternate protein names: Putative PTS system EIIA component [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLS
HKHSRCEVISGVTLPLIEQMMACRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY

Sequences:

>Translated_146_residues
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLS
HKHSRCEVISGVTLPLIEQMMACRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY
>Mature_146_residues
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLS
HKHSRCEVISGVTLPLIEQMMACRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane [H]

COG id: COG2893

COG function: function code G; Phosphotransferase system, mannose/fructose-specific component IIA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1786321, Length=146, Percent_Identity=99.3150684931507, Blast_Score=305, Evalue=9e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004701 [H]

Pfam domain/function: PF03610 EIIA-man [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16526; Mature: 16526

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS51096 PTS_EIIA_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
8.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGEG
CCCEEEEECCHHHHHHHHHHHHHCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCC
VIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMACRETMTSSEFRERIVELG
EEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPEVSSLWHQQQKNPPFVLKHNLYEY
CCHHHHHHHHHCCCCCEEECCCCCCC
>Mature Secondary Structure
MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNMLSRMMCDALHEADSGEG
CCCEEEEECCHHHHHHHHHHHHHCCCEEEHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCC
VIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMACRETMTSSEFRERIVELG
EEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GPEVSSLWHQQQKNPPFVLKHNLYEY
CCHHHHHHHHHCCCCCEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503 [H]