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The map label for this gene is ampD

Identifier: 218693576

GI number: 218693576

Start: 119037

End: 119588

Strand: Direct

Name: ampD

Synonym: EC55989_0103

Alternate gene names: 218693576

Gene position: 119037-119588 (Clockwise)

Preceding gene: 218693571

Following gene: 218693577

Centisome position: 2.31

GC content: 53.8

Gene sequence:

>552_bases
ATGTTGTTAGAACAGGGGTGGCTGGTTGGCGCGCGCCGCGTTCCCTCACCACATTACGATTGCCGCCCGGATGACGAAAC
ACCCACCCTGCTGGTGGTGCACAATATTAGCCTGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTG
GAACTATTGATCCGCAGGCACATCCTTTCTTTGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGT
GATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTGCATGGCATGCGGGAGTCTCTCAGTATCAGGGGCGCGAACG
CTGCAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTG
CGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGAT
CGGAAAACCGATCCCGGTCCTGCATTTGATTGGGCACGGTTTCGTGTGCTGGTCAGCAAGGAGACAACATGA

Upstream 100 bases:

>100_bases
GGCGAGGCGGCATGTTAAAACTCCAGATAGCTAACGAATCATAAGGTAGAAACATGCTACTCTGAACCGGGTATTAGCAC
CACATATAAGGAGATCCTGC

Downstream 100 bases:

>100_bases
CGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTT
GAAGCGTTCTTTCGGCGGGT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFGGPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSQYQGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
RKTDPGPAFDWARFRVLVSKETT

Sequences:

>Translated_183_residues
MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFGGPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSQYQGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
RKTDPGPAFDWARFRVLVSKETT
>Mature_183_residues
MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFGGPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSQYQGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
RKTDPGPAFDWARFRVLVSKETT

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family

Homologues:

Organism=Escherichia coli, GI1786300, Length=183, Percent_Identity=100, Blast_Score=381, Evalue=1e-107,
Organism=Escherichia coli, GI1787092, Length=127, Percent_Identity=39.3700787401575, Blast_Score=78, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AMPD_ECOLI (P13016)

Other databases:

- EMBL:   X15237
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   L28105
- PIR:   S05569
- RefSeq:   AP_000771.1
- RefSeq:   NP_414652.1
- ProteinModelPortal:   P13016
- SMR:   P13016
- STRING:   P13016
- EnsemblBacteria:   EBESCT00000004951
- EnsemblBacteria:   EBESCT00000014606
- GeneID:   948877
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW0106
- KEGG:   eco:b0110
- EchoBASE:   EB0039
- EcoGene:   EG10041
- eggNOG:   COG3023
- GeneTree:   EBGT00050000009506
- HOGENOM:   HBG700567
- OMA:   VQFVSCD
- ProtClustDB:   PRK11789
- BioCyc:   EcoCyc:EG10041-MONOMER
- BioCyc:   MetaCyc:EG10041-MONOMER
- Genevestigator:   P13016
- GO:   GO:0005737
- InterPro:   IPR002502
- Gene3D:   G3DSA:3.40.80.10
- SMART:   SM00644

Pfam domain/function: PF01510 Amidase_2; SSF55846 Amidase_2

EC number: =3.5.1.28

Molecular weight: Translated: 20536; Mature: 20536

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFGGPWIDALFTGTIDPQA
CCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHEEECCCCCCC
HPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQYQGRERCNDFSIGIELEGT
CHHHHHHHHHHHEEEEEEECCCCEEEEECCCCHHHHHHHHHHCCHHCCCCEEEEEEECCC
DTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPDRKTDPGPAFDWARFRVLVSK
CEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEC
ETT
CCC
>Mature Secondary Structure
MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFGGPWIDALFTGTIDPQA
CCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHEEECCCCCCC
HPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQYQGRERCNDFSIGIELEGT
CHHHHHHHHHHHEEEEEEECCCCEEEEECCCCHHHHHHHHHHCCHHCCCCEEEEEEECCC
DTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPDRKTDPGPAFDWARFRVLVSK
CEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEC
ETT
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503; 7959070