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The map label for this gene is ftsI

Identifier: 218693553

GI number: 218693553

Start: 91833

End: 93599

Strand: Direct

Name: ftsI

Synonym: EC55989_0080

Alternate gene names: 218693553

Gene position: 91833-93599 (Clockwise)

Preceding gene: 218693552

Following gene: 218693554

Centisome position: 1.78

GC content: 53.99

Gene sequence:

>1767_bases
ATGAAAGCAGCGGCGAAAACGCAGAAACCAAAACGTCAGGAAGAACATGCCAACTTTATTAGTTGGCGTTTTGCGTTGTT
ATGCGGCTGTATTCTCCTGGCGCTGGCTTTTCTGCTCGGACGCGTAGCGTGGTTACAAGTTATCTCCCCGGATATGCTGG
TGAAAGAGGGCGACATGCGTTCTCTTCGCGTTCAGCAAGTTTCCACCTCCCGCGGCATGATTACTGACCGTTCTGGTCGC
CCGTTAGCGGTGAGCGTGCCGGTAAAAGCGATTTGGGCTGACCCGAAAGAAGTGCATGACGCTGGCGGTATCAGCGTCGG
TGACCGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTTTCAGCCCGCATTAACGCCAACCCGAAAG
GGCGCTTTATTTATCTGGCGCGTCAGGTGAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCAT
CTGCGTGAAGAGTCTCGCCGTTACTATCCGTCCGGCGAAGTGACTGCTCACCTCATCGGCTTTACTAACGTCGATAGTCA
AGGGATTGAGGGCGTTGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCT
ATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGGCGCTGAGTATTGATGAACGCCTG
CAGGCGCTGGTTTATCGCGAACTGAACAACGCGGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGT
CAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCTAACAATCTGAGCGGCACGCCGAAAGAGGCGA
TGCGTAACCGTACCATCACCGACGTGTTTGAACCGGGCTCAACGGTTAAACCGATGGTGGTAATGACCGCGTTGCAACGT
GGCGTGGTGCGGGAAAACTCGGTACTCAATACCATTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCACGCTA
CAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAGCTGGCGTTAGCGATGCCGTCCT
CAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTA
TATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACA
GTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTC
CCGGTGAACGTGTCTTCCCGGAATCCATTGTCCGCACTGTGGTGCATATGATGGAAAGCGTGGCGCTACCAGGCGGCGGC
GGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTA
CATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACG
ATCCGCAGGCGGGTAAATACTACGGCGGCGCCGTTTCCGCGCCGGTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACC
ATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGGACAGGTGGCAG
ATCGTAA

Upstream 100 bases:

>100_bases
TAGCCGGGTGGAAAGGATCGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAA
AATAAGGATAAACGCGACGC

Downstream 100 bases:

>100_bases
TTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGG
CTGCGGCGGGCGATCTCTTT

Product: transpeptidase involved in septal peptidoglycan synthesis (penicillin-binding protein 3)

Products: NA

Alternate protein names: Penicillin-binding protein 3; PBP-3; Peptidoglycan glycosyltransferase 3

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAWLQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGR
PLAVSVPVKAIWADPKEVHDAGGISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIEDISSTDSQAAHNLALSIDERL
QALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMANSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQR
GVVRENSVLNTIPYRINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLGLVGERSGL
YPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVDPPVPGERVFPESIVRTVVHMMESVALPGGG
GVKAAIKGYRIAIKTGTAKKVGPDGRYINKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRT
MNIEPDALTTGDKNEFVINQGEGTGGRS

Sequences:

>Translated_588_residues
MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAWLQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGR
PLAVSVPVKAIWADPKEVHDAGGISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIEDISSTDSQAAHNLALSIDERL
QALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMANSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQR
GVVRENSVLNTIPYRINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLGLVGERSGL
YPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVDPPVPGERVFPESIVRTVVHMMESVALPGGG
GVKAAIKGYRIAIKTGTAKKVGPDGRYINKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRT
MNIEPDALTTGDKNEFVINQGEGTGGRS
>Mature_588_residues
MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAWLQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGR
PLAVSVPVKAIWADPKEVHDAGGISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIEDISSTDSQAAHNLALSIDERL
QALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMANSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQR
GVVRENSVLNTIPYRINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLGLVGERSGL
YPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVDPPVPGERVFPESIVRTVVHMMESVALPGGG
GVKAAIKGYRIAIKTGTAKKVGPDGRYINKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRT
MNIEPDALTTGDKNEFVINQGEGTGGRS

Specific function: Cell wall formation. Essential for the formation of a septum of the murein sacculus. Synthesis of cross-linked peptidoglycan from the lipid intermediates

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side. Note=The bulk of the molecule, except for the N-terminal membrane anchor region, protrudes into the periplasmic space, where it acts on murein (By similarity)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family

Homologues:

Organism=Escherichia coli, GI1786272, Length=588, Percent_Identity=100, Blast_Score=1202, Evalue=0.0,
Organism=Escherichia coli, GI1786854, Length=636, Percent_Identity=25, Blast_Score=139, Evalue=7e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FTSI_ECO57 (P0AD69)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   H85490
- PIR:   H90639
- RefSeq:   NP_285780.1
- RefSeq:   NP_308115.1
- ProteinModelPortal:   P0AD69
- SMR:   P0AD69
- MINT:   MINT-1031936
- EnsemblBacteria:   EBESCT00000024576
- EnsemblBacteria:   EBESCT00000058256
- GeneID:   913535
- GeneID:   956770
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0094
- KEGG:   ecs:ECs0088
- GeneTree:   EBGT00050000010128
- HOGENOM:   HBG712588
- OMA:   QASFVSW
- ProtClustDB:   PRK15105
- BioCyc:   ECOL83334:ECS0088-MONOMER
- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase; SSF56519 PBP_dimer; SSF56601 PBP_transp_fold

EC number: =2.4.1.129

Molecular weight: Translated: 63878; Mature: 63878

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: ACT_SITE 307-307

Signals:

None

Transmembrane regions:

HASH(0x171be384)-;

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAWLQVISPDMLVKEGDMR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCH
SLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAGGISVGDRWKALANALNIP
HHHHHHHHCCCCEEECCCCCCEEEECCCCEEECCHHHHHCCCCCCCCHHHHHHHHHHCCC
LDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIHLREESRRYYPSGEVTAHLIG
HHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEEEE
FTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIEDISSTDSQAAHNLALSIDERL
EECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEHHHHH
QALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMANSPSYNPNNLSGTPKEAMRNRTIT
HHHHHHHHHCCEEECCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCH
DVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPYRINGHEIKDVARYSELTLTGVLQKSS
HHCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCEEECCCHHHHHHHHHHHHHEEEECCCC
NVGVSKLALAMPSSALVDTYSRFGLGKATNLGLVGERSGLYPQKQRWSDIERATFSFGYG
CCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCC
LMVTPLQLARVYATIGSYGIYRPLSITKVDPPVPGERVFPESIVRTVVHMMESVALPGGG
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
GVKAAIKGYRIAIKTGTAKKVGPDGRYINKYIAYTAGVAPASQPRFALVVVINDPQAGKY
CCCEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCC
YGGAVSAPVFGAIMGGVLRTMNIEPDALTTGDKNEFVINQGEGTGGRS
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCH
>Mature Secondary Structure
MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAWLQVISPDMLVKEGDMR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCH
SLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAGGISVGDRWKALANALNIP
HHHHHHHHCCCCEEECCCCCCEEEECCCCEEECCHHHHHCCCCCCCCHHHHHHHHHHCCC
LDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIHLREESRRYYPSGEVTAHLIG
HHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEEEE
FTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIEDISSTDSQAAHNLALSIDERL
EECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHEEEHHHHH
QALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMANSPSYNPNNLSGTPKEAMRNRTIT
HHHHHHHHHCCEEECCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCH
DVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPYRINGHEIKDVARYSELTLTGVLQKSS
HHCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCEEECCCHHHHHHHHHHHHHEEEECCCC
NVGVSKLALAMPSSALVDTYSRFGLGKATNLGLVGERSGLYPQKQRWSDIERATFSFGYG
CCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCC
LMVTPLQLARVYATIGSYGIYRPLSITKVDPPVPGERVFPESIVRTVVHMMESVALPGGG
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
GVKAAIKGYRIAIKTGTAKKVGPDGRYINKYIAYTAGVAPASQPRFALVVVINDPQAGKY
CCCEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCC
YGGAVSAPVFGAIMGGVLRTMNIEPDALTTGDKNEFVINQGEGTGGRS
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796