Click here to switch to the map view.

The map label for this gene is fruR

Identifier: 218693549

GI number: 218693549

Start: 88448

End: 89452

Strand: Direct

Name: fruR

Synonym: EC55989_0076

Alternate gene names: 218693549

Gene position: 88448-89452 (Clockwise)

Preceding gene: 218693548

Following gene: 218693550

Centisome position: 1.72

GC content: 54.93

Gene sequence:

>1005_bases
GTGAAACTGGATGAAATCGCTCGGCTGGCGGGAGTGTCGCGGACCACTGCAAGCTATGTTATTAACGGCAAAGCGAAGCA
ATACCGTGTGAGCGACAAAACCGTTGAAAAAGTCATGGCTGTGGTGCGTGAGCACAATTACCACCCGAACGCCGTGGCAG
CTGGGCTTCGTGCTGGACGCACACGTTCTATTGGTCTTGTGATCCCCGATCTGGAGAACACCAGCTATACCCGCATCGCT
AACTATCTTGAGCGCCAGGCGCGGCAACGGGGTTATCAACTGCTGATTGCCTGCTCAGAAGATCAGCCAGACAACGAAAT
GCGCTGCATTGAGCACCTTTTACAGCGTCAGGTTGATGCCATTATTGTTTCGACGTCGTTGCCACCTGAGCATCCTTTTT
ATCAACGCTGGGCTAACGACCCGTTCCCGATTGTCGCGCTGGACCGCGCCCTCGATCGTGAACATTTCACCAGCGTGGTT
GGTGCCGATCAGGATGATGCCGAAATGCTGGCGGAAGAGTTACGTAAGTTTCCCGCCGAGACGGTGCTTTATCTTGGCGC
GCTGCCGGAGCTTTCTGTCAGCTTCCTGCGTGAACAAGGTTTCCGTACTGCCTGGAAAGATGATCCGCGCGAAGTGCATT
TCCTGTATGCCAACAGCTATGAGCGGGAGGCGGCTGCCCAGTTATTCGAAAAATGGCTGGAAACGCATCCGATGCCGCAG
GCGCTGTTCACAACGTCGTTTGCGTTGTTGCAAGGGGTGATGGATGTCACGCTACGTCGCGATGGTAAACTGCCTTCTGA
CCTGGCAATTGCCACCTTTGGCGATAACGAACTGCTCGACTTCTTACAGTGCCCGGTGCTGGCAGTGGCTCAACGTCACC
GCGATGTCGCAGAGCGTGTGCTGGAGATTGTCCTGGCAAGCCTGGACGAACCGCGTAAGCCAAAACCTGGTTTAACGCGC
ATTAAACGTAATCTCTATCGCCGCGGTGTGCTCAGCCGTAGCTAA

Upstream 100 bases:

>100_bases
AAGCAGTTGCCGCAGTTAATTTTCTGCGCTTAGATGTTAATGAATTTAACCCATACCAGTACAATGGCTATGGTTTTTAC
ATTTTACGCAAGGGGCAATT

Downstream 100 bases:

>100_bases
GCCGCGAACAAAAATACGCGCCAGGTGAATTTCCCTCTGGCGCGTAGAGTACGGGAATGGACATCAATATCCTTAAAGTA
AATAAGACTATTCCTGACTA

Product: DNA-binding transcriptional regulator FruR

Products: NA

Alternate protein names: Catabolite repressor/activator

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIA
NYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAAQLFEKWLETHPMPQ
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTR
IKRNLYRRGVLSRS

Sequences:

>Translated_334_residues
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIA
NYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAAQLFEKWLETHPMPQ
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTR
IKRNLYRRGVLSRS
>Mature_334_residues
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIA
NYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAAQLFEKWLETHPMPQ
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTR
IKRNLYRRGVLSRS

Specific function: Represses some operons but activates others. It is implicated in the regulation of a large number of operons encoding enzymes which comprise central pathways of carbon metabolism. Binds D-fructose as an inducer

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1786268, Length=334, Percent_Identity=100, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1790194, Length=319, Percent_Identity=27.5862068965517, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787580, Length=289, Percent_Identity=23.5294117647059, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=22.2222222222222, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1790369, Length=312, Percent_Identity=23.0769230769231, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790715, Length=284, Percent_Identity=26.056338028169, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1789068, Length=304, Percent_Identity=24.6710526315789, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1789202, Length=128, Percent_Identity=32.03125, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788474, Length=207, Percent_Identity=26.5700483091787, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FRUR_ECO57 (P0ACP3)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85490
- PIR:   D90639
- RefSeq:   NP_285776.1
- RefSeq:   NP_308111.1
- ProteinModelPortal:   P0ACP3
- MINT:   MINT-1252410
- EnsemblBacteria:   EBESCT00000025852
- EnsemblBacteria:   EBESCT00000058150
- GeneID:   913527
- GeneID:   956764
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0090
- KEGG:   ecs:ECs0084
- GeneTree:   EBGT00050000009239
- HOGENOM:   HBG298357
- OMA:   ADSAMEL
- ProtClustDB:   PRK11303
- BioCyc:   ECOL83334:ECS0084-MONOMER
- GO:   GO:0005622
- InterPro:   IPR012781
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- SMART:   SM00354
- TIGRFAMs:   TIGR02417

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 38000; Mature: 38000

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGR
CCHHHHHHHHCCCHHHHHHEECCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
TRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDA
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHE
IIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVVGADQDDAEMLAEELRKFPAE
EEEECCCCCCCCHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHH
TVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAAQLFEKWLETHPMPQ
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCH
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVREHNYHPNAVAAGLRAGR
CCHHHHHHHHCCCHHHHHHEECCCCCEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
TRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQPDNEMRCIEHLLQRQVDA
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHE
IIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVVGADQDDAEMLAEELRKFPAE
EEEECCCCCCCCHHHHHCCCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHH
TVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYANSYEREAAAQLFEKWLETHPMPQ
HHHHHHCCHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCH
ALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDNELLDFLQCPVLAVAQRHRDVAERV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
LEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796