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The map label for this gene is ilvI

Identifier: 218693547

GI number: 218693547

Start: 86050

End: 87774

Strand: Direct

Name: ilvI

Synonym: EC55989_0074

Alternate gene names: 218693547

Gene position: 86050-87774 (Clockwise)

Preceding gene: 218693546

Following gene: 218693548

Centisome position: 1.67

GC content: 52.87

Gene sequence:

>1725_bases
ATGGAGATGTTGTCTGGAGCCGAGATGGTCGTCCGATCGCTTATCGATCAGGGCGTTAAACAAGTATTCGGTTATCCCGG
AGGCGCAGTCCTTGATATTTACGATGCATTGCATACCGTGGGTGGTATCGATCATGTTTTAGTTCGTCATGAGCAGGCGG
CAGTGCATATGGCCGATGGTCTGGCGCGCGCGACCGGGGAAGTCGGCGTCGTGCTGGTAACGTCGGGTCCAGGGGCGACC
AATGCGATTACCGGCATCGCCACCGCTTATATGGATTCCATTCCATTAGTTGTCCTTTCCGGGCAGGTAGCGACCTCGTT
GATAGGTTACGATGCCTTTCAGGAGTGCGACATGGTGGGGATTTCGCGACCGGTGGTTAAACACAGTTTTCTGGTTAAGC
AAACGGAAGACATTCCGCAGGTGCTGAAAAAGGCTTTCTGGCTGGCGGCAAGTGGTCGCCCAGGACCAGTGGTCGTTGAT
TTACCGAAAGATATTCTTAATCCGGCGAACAAATTACCCTATGTCTGGCCTGAGTCGGTCAGTATGCGTTCTTACAATCC
CACTACTACCGGACATAAAGGGCAAATTAAGCGCGCTCTGCAAACGCTGGTAGCGGCAAAAAAACCGGTTGTCTACGTAG
GCGGTGGGGCAATCATGGCGGGCTGCCATCAGCAGTTGAAAGAAACGGTGGAGGCGTTGAATCTGCCCGTTGTTTCCTCA
TTGATGGGGCTGGGGGCGTTTCCGGCAACGCATCGTCAGGCGCTGGGCATGCTGGGAATGCACGGTACCTACGAAGCCAA
TATGACGATGCATAACGCGGATGTGATTTTCGCCGTCGGGGTACGATTTGATGACCGAACGACGAACAATCTGGCAAAGT
ACTGCCCAAATGCCACTGTTCTGCATATCGATATTGATCCTACTTCCATTTCTAAAACCGTGACTGCGGATATCCCGATT
GTGGGGGATGCTCGCCAGGTCCTCGAACAAATGCTTGAACTCTTGTCGCAAGAATCCGCCCATCAACCACTGGATGAGAT
CCGCGACTGGTGGCAGCAAATTGAACAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAAC
CGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTCGGGCAGCACCAGATG
TTTGCTGCACTTTATTATCCATTCGACAAACCGCGTCGCTGGATCAATTCCGGTGGCCTCGGCACGATGGGTTTTGGTTT
ACCTGCGGCACTGGGCGTCAAAATGGCGTTGCCAGAAGAAACCGTGGTTTGCGTCACTGGCGACGGCAGTATTCAGATGA
ACATCCAGGAACTGTCTACCGCGTTGCAATACGAGTTGCCGGTACTGGTGGTGAATCTCAATAACCGCTATCTGGGGATG
GTGAAACAGTGGCAGGACATGATCTATTCCGGCCGTCATTCACAATCTTATATGCAATCACTACCCGATTTCGTCCGTCT
GGCGGAAGCCTATGGTCACGTCGGGATCCAGATCTCTCATCCGCATGAGCTGGAAAGCAAACTTAGCGAGGCGCTGGAAC
AGGTGTGCAATAATCGCCTGGTGTTTGTTGATGTTACCGTCGATGGCAGTGAGCACGTCTACCCGATGCAGATTCGCGGG
GGCGGAATGGATGAAATGTGGTTAAGCAAAACGGAGAGAACCTGA

Upstream 100 bases:

>100_bases
TTATTACCCTGCGTTTATGTCTCTGGCTGCCAATTGCTTAAGCAAGATCGGACGGTTAATGTGTTTTACACATTTTTTCC
GTCAAACAGTGAGGCAGGCC

Downstream 100 bases:

>100_bases
TTATGCGCCGGATATTATCAGTCTTACTCGAAAATGAATCAGGCGCGTTATCCCGCGTGATTGGCCTTTTTTCCCAGCGT
GGCTACAACATTGAAAGCCT

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS-III; ALS-III; Acetohydroxy-acid synthase III large subunit [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAIMAGCHQQLKETVEALNLPVVSS
LMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPI
VGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRG
GGMDEMWLSKTERT

Sequences:

>Translated_574_residues
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAIMAGCHQQLKETVEALNLPVVSS
LMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPI
VGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRG
GGMDEMWLSKTERT
>Mature_574_residues
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGAT
NAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAIMAGCHQQLKETVEALNLPVVSS
LMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPI
VGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVTSDVGQHQM
FAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRG
GGMDEMWLSKTERT

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=565, Percent_Identity=27.9646017699115, Blast_Score=192, Evalue=6e-49,
Organism=Homo sapiens, GI21361361, Length=580, Percent_Identity=25.6896551724138, Blast_Score=164, Evalue=2e-40,
Organism=Escherichia coli, GI87081685, Length=574, Percent_Identity=99.4773519163763, Blast_Score=1179, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=566, Percent_Identity=39.2226148409894, Blast_Score=410, Evalue=1e-115,
Organism=Escherichia coli, GI1786717, Length=568, Percent_Identity=33.9788732394366, Blast_Score=315, Evalue=7e-87,
Organism=Escherichia coli, GI1787096, Length=561, Percent_Identity=30.8377896613191, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1788716, Length=556, Percent_Identity=24.6402877697842, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17531299, Length=547, Percent_Identity=27.6051188299817, Blast_Score=174, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17531301, Length=561, Percent_Identity=27.0944741532977, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17542570, Length=576, Percent_Identity=25.6944444444444, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6323755, Length=583, Percent_Identity=42.8816466552316, Blast_Score=445, Evalue=1e-126,
Organism=Saccharomyces cerevisiae, GI6320816, Length=478, Percent_Identity=24.8953974895398, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI19922626, Length=508, Percent_Identity=28.5433070866142, Blast_Score=190, Evalue=2e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 62946; Mature: 62946

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVG
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCC
ISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVDLPKDILNPANKLPYVWPESV
CCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEECCHHHCCHHHCCCEECCCCC
SMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAIMAGCHQQLKETVEALNLPVVSS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHH
LMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATV
HHHCCCCCHHHHHHHHHCCCCCCEECCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEE
LHIDIDPTSISKTVTADIPIVGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRAR
EEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QCLKYDTHSEKIKPQAVIETLWRLTKGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGL
HHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHEECCCHHHHHHHCCCCC
GTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHEECCEEEEECCCCHHHH
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPHELESKLSEALEQVCNNRL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCE
VFVDVTVDGSEHVYPMQIRGGGMDEMWLSKTERT
EEEEEEECCCCCEEEEEECCCCCCHHHHHHCCCC
>Mature Secondary Structure
MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGIDHVLVRHEQAAVHMADG
CCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
LARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQVATSLIGYDAFQECDMVG
HHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCCCCC
ISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVDLPKDILNPANKLPYVWPESV
CCCHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEECCHHHCCHHHCCCEECCCCC
SMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAIMAGCHQQLKETVEALNLPVVSS
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHH
LMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVIFAVGVRFDDRTTNNLAKYCPNATV
HHHCCCCCHHHHHHHHHCCCCCCEECCEEEECCCEEEEEEEEECCCCHHHHHHHCCCCEE
LHIDIDPTSISKTVTADIPIVGDARQVLEQMLELLSQESAHQPLDEIRDWWQQIEQWRAR
EEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
QCLKYDTHSEKIKPQAVIETLWRLTKGDAYVTSDVGQHQMFAALYYPFDKPRRWINSGGL
HHHHCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHEECCCHHHHHHHCCCCC
GTMGFGLPAALGVKMALPEETVVCVTGDGSIQMNIQELSTALQYELPVLVVNLNNRYLGM
CCCCCCCHHHHCEEEECCCCEEEEEECCCCEEEEHHHHHHHHHEECCEEEEECCCCHHHH
VKQWQDMIYSGRHSQSYMQSLPDFVRLAEAYGHVGIQISHPHELESKLSEALEQVCNNRL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHCCCE
VFVDVTVDGSEHVYPMQIRGGGMDEMWLSKTERT
EEEEEEECCCCCEEEEEECCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6308579; 1630901; 9278503; 3891724; 9298646 [H]