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The map label for this gene is djlA

Identifier: 218693527

GI number: 218693527

Start: 59291

End: 60106

Strand: Direct

Name: djlA

Synonym: EC55989_0055

Alternate gene names: 218693527

Gene position: 59291-60106 (Clockwise)

Preceding gene: 218693520

Following gene: 218693534

Centisome position: 1.15

GC content: 52.82

Gene sequence:

>816_bases
ATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTTTGGGGCGTAGTGTTAGGCCT
GTTAATTGGCCATATGTTTGATAAAGCCCGTAGCCGTAAAATGGCGTGGTTCGCCAACCAGCGTGAGCGTCAGGCGCTGT
TTTTTGCCACCACTTTTGAAGTGATGGGGCATTTAACCAAATCCAAAGGTCGCGTCACGGAGGCTGATATTCATATCGCC
AGCCAGTTGATGGACCGAATGAATCTTCATGGCGCTTCCCGTACTGCGGCGCAAAATGCGTTCCGGGTGGGAAAATCAGA
CAATTACCCGCTGCGCGAAAAGATGCGCCAGTTTCGCAGTGTCTGCTTTGGTCGTTTTGACTTAATTCGTATGTTTCTGG
AGATCCAGATTCAGGCGGCGTTTGCTGATGGTTCACTGCACCCGAATGAACGGGCGGTGCTGTATGTCATTGCTGAAGAA
TTAGGGATCTCCCGCGCCCAGTTTGACCAGTTTTTGCGCATGATGCAGGGCGGTGCACAGTTTGGCGGCGGTTATCAGCA
GCAATCTGGCGGTGGTAACTGGCAGCAAGCGCAGCGTGGCCCGACGCTGGAAGATGCCTGTAATGTGCTGGGCGTGAAGC
CGACGGATGATGCGACCACCATCAAACGTGCCTACCGTAAGCTGATGAGTGAACACCATCCCGATAAGCTGGTGGCGAAA
GGTTTGCCGCCTGAGATGATGGAGATGGCGAAGCAGAAAGCGCAGGAAATTCAGCAGGCATATGAGCTGATAAAGCAGCA
GAAAGGGTTTAAATAA

Upstream 100 bases:

>100_bases
ATAGGCGATGTCCCACAATTGACCGCAGCCGGAAAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCG
TGTGTCGATTTGGGGAATAT

Downstream 100 bases:

>100_bases
GTTTTAAACTGTAGGCCTGATAAGACGCGGCCCGCGTCGCATCAGGCAATCGGCGCACAATTGCCGGATGCGGCGTGAAC
GCCTTATCCGCCTACCGAAC

Product: Dna-J like membrane chaperone protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWFANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIA
SQLMDRMNLHGASRTAAQNAFRVGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDATTIKRAYRKLMSEHHPDKLVAK
GLPPEMMEMAKQKAQEIQQAYELIKQQKGFK

Sequences:

>Translated_271_residues
MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWFANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIA
SQLMDRMNLHGASRTAAQNAFRVGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDATTIKRAYRKLMSEHHPDKLVAK
GLPPEMMEMAKQKAQEIQQAYELIKQQKGFK
>Mature_271_residues
MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWFANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIA
SQLMDRMNLHGASRTAAQNAFRVGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDATTIKRAYRKLMSEHHPDKLVAK
GLPPEMMEMAKQKAQEIQQAYELIKQQKGFK

Specific function: Regulatory dnaK co-chaperone. Direct interaction between dnaK and djlA is needed for the induction of the wcaABCDE operon, involved in the synthesis of a colanic acid polysaccharide capsule, possibly through activation of the rcsB/rcsC phosphotransfer sig

COG id: COG1076

COG function: function code O; DnaJ-domain-containing proteins 1

Gene ontology:

Cell location: Cell inner membrane; Single-pass type III membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 J domain

Homologues:

Organism=Escherichia coli, GI1786241, Length=271, Percent_Identity=99.6309963099631, Blast_Score=560, Evalue=1e-161,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DJLA_ECO57 (Q7AHS5)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D85487
- PIR:   D90636
- RefSeq:   NP_285752.1
- RefSeq:   NP_308087.1
- ProteinModelPortal:   Q7AHS5
- SMR:   Q7AHS5
- EnsemblBacteria:   EBESCT00000024867
- EnsemblBacteria:   EBESCT00000060099
- GeneID:   913460
- GeneID:   956731
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0064
- KEGG:   ecs:ECs0060
- GeneTree:   EBGT00050000010193
- HOGENOM:   HBG508481
- OMA:   LRACWRM
- ProtClustDB:   PRK09430
- BioCyc:   ECOL83334:ECS0060-MONOMER
- HAMAP:   MF_01153
- InterPro:   IPR001623
- InterPro:   IPR007791
- InterPro:   IPR015609
- InterPro:   IPR003095
- Gene3D:   G3DSA:1.10.287.110
- PANTHER:   PTHR11821
- PRINTS:   PR00625
- SMART:   SM00271

Pfam domain/function: PF00226 DnaJ; PF05099 TerB; SSF46565 DnaJ_N

EC number: NA

Molecular weight: Translated: 30566; Mature: 30566

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS00636 DNAJ_1; PS50076 DNAJ_2

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10fae7c0)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWFANQRERQALFFATTFE
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
VMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFRVGKSDNYPLREKMRQFRS
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEELGISRAQFDQFLRMMQGGAQ
HHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHHCCHH
FGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDATTIKRAYRKLMSEHHPDKLVAK
CCCCCCCCCCCCCHHHHHCCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHC
GLPPEMMEMAKQKAQEIQQAYELIKQQKGFK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure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CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796