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The map label for this gene is amiB [H]

Identifier: 218692503

GI number: 218692503

Start: 4913830

End: 4915176

Strand: Direct

Name: amiB [H]

Synonym: ECED1_4954

Alternate gene names: 218692503

Gene position: 4913830-4915176 (Clockwise)

Preceding gene: 218692502

Following gene: 218692504

Centisome position: 94.32

GC content: 54.12

Gene sequence:

>1347_bases
TTGAAAGGTGGCGGGATGATGTATCGCATCAGAAATTGGTTGGTAGCGACGTTGCTGCTGCTGTGCGCGCCGGTGGGTGC
CGCGACGCTCTCTGATATTCAGGTTTCTAACGGTAATCAACAGGCACGGATAACGTTGAGTTTTATTGGCGATCCTGATT
ATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGGGTAATTCAGGGGCTGCCGTTGTTG
TTTAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCT
CACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCTATTACACTGTTGTCTTTACGATTAACGCCGATG
CGCCGCCATCGCCTCCGCCAATGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCG
CGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATCAGCAGTAATACGGTAACGCGTCCGGCAGCGCGCGC
GACGGCTAACACAGGCGATAAAATTATCATCGCTATTGATGCCGGACACGGCGGTCAGGACCCTGGCGCTATCGGCCCTG
GTGGTACACGGGAGAAAAATGTCACCATCGCCATTGCGCGTAAATTACGTACCTTGCTCAATAACGATCCGATGTTTAAG
GGCGTTTTAACCCGTGACGGGGATTATTTTATCTCGGTGATGGGGCGCAGTGATGTGGCACGTAAGCAAAACGCCAATTT
CCTTGTGTCGATTCATGCCGATGCCGCACCAAACCGTAGTGCCACCGGTGCCTCTGTATGGGTGCTCTCTAACCGTCGCG
CCAACAGCGAAATGGCAAGCTGGCTGGAGCAGCACGAGAAACAGTCGGAGCTACTGGGCGGAGCGGGCGATGTGCTGGCG
AACAGTCAGTCTGACCCCTATTTAAGCCAGGCGGTGCTGGATTTACAGTTCGGTCATTCCCAGCGGGTAGGGTATGATGT
AGCGACCAGTATGATCAATCAGTTGCAACGCATTGGCGAAATACATAAACGCCGACCAGAACACGCGAGTCTCGGCGTTC
TGCGTTCGCCGGATATTCCGTCAGTACTGGTCGAAACCGGTTTTATCAGTAACAACAGCGAAGAACGTTTGCTGGCGAGC
GACGATTACCAACAACAGCTGGCAGAAGCCATTTACAAAGGCCTGCGCAATTACTTCCTTGCGCATCCGATGCAATCTGC
GCCGCAGGGTGCAACGGCACAAACGGCCAGTACGGTGACGACGCCAGATCGCACGCTGCCAAACTAA

Upstream 100 bases:

>100_bases
CGAAATACACATTGATTATCAGGCACAAGGCCGTGAGGCGCGCGTGAGTGCGGTTTCCTCTGCGGGTGAATTGTTGCTGG
CGCGTTTAGCCGGTTAACCT

Downstream 100 bases:

>100_bases
GGACGATTGATGCCAATTCAGGTCTTACCGCCACAACTGGCGAACCAGATTGCCGCAGGTGAGGTGGTCGAGCGACCTGC
GTCGGTAGTCAAAGAACTGG

Product: N-acetylmuramoyl-l-alanine amidase II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MKGGGMMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQSKRTVALDIKQTGVIQGLPLL
FSGNNLVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQNGSYYTVVFTINADAPPSPPPMVAKRVETPAVVAPRVSEPA
RNPFKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFK
GVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLA
NSQSDPYLSQAVLDLQFGHSQRVGYDVATSMINQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLAS
DDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN

Sequences:

>Translated_448_residues
MKGGGMMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQSKRTVALDIKQTGVIQGLPLL
FSGNNLVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQNGSYYTVVFTINADAPPSPPPMVAKRVETPAVVAPRVSEPA
RNPFKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFK
GVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLA
NSQSDPYLSQAVLDLQFGHSQRVGYDVATSMINQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLAS
DDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN
>Mature_448_residues
MKGGGMMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQSKRTVALDIKQTGVIQGLPLL
FSGNNLVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQNGSYYTVVFTINADAPPSPPPMVAKRVETPAVVAPRVSEPA
RNPFKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFK
GVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLA
NSQSDPYLSQAVLDLQFGHSQRVGYDVATSMINQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLAS
DDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division. Can also act as powerful autolysin in the presence of murein synthesis inhibitors [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=445, Percent_Identity=97.752808988764, Blast_Score=870, Evalue=0.0,
Organism=Escherichia coli, GI87082163, Length=225, Percent_Identity=40.8888888888889, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1788776, Length=244, Percent_Identity=38.5245901639344, Blast_Score=174, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 48261; Mature: 48261

Theoretical pI: Translated: 9.92; Mature: 9.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGGGMMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQS
CCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCCCCCCC
KRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQN
CCEEEEEECCCCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHCC
GSYYTVVFTINADAPPSPPPMVAKRVETPAVVAPRVSEPARNPFKTESNRTTGVISSNTV
CCEEEEEEEEECCCCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCCCCCCEEEEEECCCC
TRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFK
CCCHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCEE
GVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMAS
EEEECCCCEEEEEECCCHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCHHHHH
WLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFGHSQRVGYDVATSMINQLQRIGE
HHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
IHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLASDDYQQQLAEAIYKGLRNYFL
HHHCCCCCCCCCEEECCCCCCHHHEECCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHH
AHPMQSAPQGATAQTASTVTTPDRTLPN
HCCCCCCCCCCCCCCCCEECCCCCCCCC
>Mature Secondary Structure
MKGGGMMYRIRNWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFIGDPDYAFSHQS
CCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEEEECCCCCCCCCCC
KRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDLTENGKTEAVKRQN
CCEEEEEECCCCCCCCCCEEEECCHHHHHHHCCCCCCCEEEEEEEEECCCCCCHHHHHCC
GSYYTVVFTINADAPPSPPPMVAKRVETPAVVAPRVSEPARNPFKTESNRTTGVISSNTV
CCEEEEEEEEECCCCCCCCCHHHHHCCCCCEECCCCCCCCCCCCCCCCCCEEEEEECCCC
TRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIAIARKLRTLLNNDPMFK
CCCHHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCCCCEE
GVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSATGASVWVLSNRRANSEMAS
EEEECCCCEEEEEECCCHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEEECCCCCHHHHH
WLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFGHSQRVGYDVATSMINQLQRIGE
HHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
IHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLLASDDYQQQLAEAIYKGLRNYFL
HHHCCCCCCCCCEEECCCCCCHHHEECCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHH
AHPMQSAPQGATAQTASTVTTPDRTLPN
HCCCCCCCCCCCCCCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7511774; 7610040; 9278503; 1594459 [H]