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The map label for this gene is nrfE [H]

Identifier: 218692366

GI number: 218692366

Start: 4763371

End: 4765029

Strand: Direct

Name: nrfE [H]

Synonym: ECED1_4802

Alternate gene names: 218692366

Gene position: 4763371-4765029 (Clockwise)

Preceding gene: 218692365

Following gene: 218692367

Centisome position: 91.44

GC content: 56.3

Gene sequence:

>1659_bases
GTGTTGACCCCGTTGACGGCCTTCGCGGGAGTGCGGTTGCGCTGGCCTGCCATGATGCGACTCACTTGCATCGGCATTCT
GGCGCAGTTCGCGCTCCTGCTGCTCGCCTTTGGCGTACTGACGTATTGTTTTCTCATCAGCGATTTCTCGGTCATTTATG
TCGCCCAACATAGCTATAGCCTGCTGTCGTGGGAACTCAAACTGGCGGCGGTGTGGGGCGGTCATGAAGGTTCGCTGCTG
CTTTGGGTGCTGCTGCTTTCCGCCTGGAGCGCGCTGTTTGCCTGGCATTATCGACAGCAAACCGATCCGCTATTTCCGCT
CACGTTAACGGTTTTATCGCTGATACTTGCCGCACTGCTGCTGTTTGTTGTCGGATGGTCCGATCCTTTTGTGAGGATAT
TTCCGCCCGCAATAGAAGGGCGCGATCTCAACCCAATGCTGCAACATCCCGGTCTTATCTTTCATCCACCGCTGCTTTAT
CTCGGCTACGGCGGTTTGATGGTGGCGGCGAGCGTGGCGCTGGCGAGTTTACTGCGCGGCGAGTTTGATGGTACCTGCGC
CCGAATTTGCTGGCGTTGGGCACTACCTGGCTGGAGTGCATTAACCGCAGGGATCATTCTCGGTTCCTGGTGGGCTTATT
GCGAACTGGGCTGGGGCGGTTGGTGGTTTTGGGATCCGGTTGAAAACGCGTCGTTATTACCCTGGCTTTCATCTACTGCG
TTGCTACACAGTTTGTCTTTGACACGCCAGCGGGGGATTTTCCGCCACTGGTCGCTGTTGCTGGCGATAGTAACTCTGAT
GCTGTCGCTGCTGGGCACCTTAATTGTCCGTTCTGGCATTCTGGTTTCGGTTCATGCGTTCGCGCTGGATAACGTCCGCG
CCGTGCCGTTGTTCAGCCTGTTTGCACTGATTAGCCTTGCGTCTCTGGCTCTGTATGGCTGGCGAGCGCGGGACGGTGGC
CCGGCGGTGCGTTTTTCGGGGTTATCGCGGGAAATGTTAATCCTCGCTACGCTGTTGCTGTTTTGCGCAGTGCTACTGAT
CGTGCTGGTGGGAACGCTTTATCCGATGATTTACGGCCTGCTGGGCTGGGGACGCCTCTCCGTTGGCGCGCCGTATTTTA
ACCGCGCAACGTTGCCGTTTGCTCTGTTGATGCTGGTGGTGATTGTGCTGGCGACGTTTGTCTCTGGCAAACGTGCTCAG
CTTCCGGCGCTGCTGGCGCATGCGGGCGTGCTGTTATTTGCCGCGGGGATCGTGGTTTCCAGCGTCAGCCGTCAGGAGAT
CAGCCTGAATTTACAGCCGGGTCAGCAGGTGACGCTGGCAGGATACACCTTCCGTTTTGAGCGCCTCGATCTGCAAGCCA
AAGGCAATTACACCAGCGAAAAAGCGATAGTGGCACTGTTTGACCATCAGCAACGCATTGGTGAATTAACGCCGGAGCGG
CGTTTTTACGAAGCACGTCGTCAGCAAATGATGGAACCGTCAATTCGCTGGAATGGCATCCATGACTGGTATGCGGTCAT
GGGCGAGAAAACCGGAGCGGATCGTTACGCTTTTCGTTTGTATGTACAAAGCGGTGTGCGCTGGATCTGGGGGGGAGGAT
TGTTGATGATTTCGGGCGCATTGTTAAGCGGATGGCGGGGAAGGAAGCGCGATGAATAA

Upstream 100 bases:

>100_bases
GGGCAGTTAACGGTGGCGTAAGCCAGAACAGAGGTGGTTTCTGGACGTATTCCTTCCTGAAGTCGGTTTTCTGGCGTTGT
TGTTAAGTCTCGGGGTCAAC

Downstream 100 bases:

>100_bases
AGGGCTTCTCACGCTACTGCTGTTATTTACCTGTTTTGCGCGCGCTCAGGTTGTAGACACCTGGCAATTCGCCAATCCGC
AACAACAGGAACAGGCGTTA

Product: heme lyase subunit NrfE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLL
LWVLLLSAWSALFAWHYRQQTDPLFPLTLTVLSLILAALLLFVVGWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLY
LGYGGLMVAASVALASLLRGEFDGTCARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSSTA
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGWRARDGG
PAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFALLMLVVIVLATFVSGKRAQ
LPALLAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPER
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMISGALLSGWRGRKRDE

Sequences:

>Translated_552_residues
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLL
LWVLLLSAWSALFAWHYRQQTDPLFPLTLTVLSLILAALLLFVVGWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLY
LGYGGLMVAASVALASLLRGEFDGTCARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSSTA
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGWRARDGG
PAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFALLMLVVIVLATFVSGKRAQ
LPALLAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPER
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMISGALLSGWRGRKRDE
>Mature_552_residues
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLL
LWVLLLSAWSALFAWHYRQQTDPLFPLTLTVLSLILAALLLFVVGWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLY
LGYGGLMVAASVALASLLRGEFDGTCARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSSTA
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGWRARDGG
PAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPYFNRATLPFALLMLVVIVLATFVSGKRAQ
LPALLAHAGVLLFAAGIVVSSVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPER
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMISGALLSGWRGRKRDE

Specific function: May be required for the biogenesis of c-type cytochromes. Possible subunit of a heme lyase [H]

COG id: COG1138

COG function: function code O; Cytochrome c biogenesis factor

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ccmF/cycK/ccl1/nrfE/ccsA family [H]

Homologues:

Organism=Escherichia coli, GI1790511, Length=552, Percent_Identity=97.2826086956522, Blast_Score=997, Evalue=0.0,
Organism=Escherichia coli, GI1788524, Length=584, Percent_Identity=38.527397260274, Blast_Score=338, Evalue=5e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002541
- InterPro:   IPR003567
- InterPro:   IPR003568
- InterPro:   IPR003570 [H]

Pfam domain/function: PF01578 Cytochrom_C_asm [H]

EC number: NA

Molecular weight: Translated: 61455; Mature: 61455

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYS
CCCCCHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCE
LLSWELKLAAVWGGHEGSLLLWVLLLSAWSALFAWHYRQQTDPLFPLTLTVLSLILAALL
EEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
LFVVGWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRG
HHHHCCCCCCEEEECCCCCCCCCCHHHHCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHC
EFDGTCARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSSTA
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCHHHHEEECCCCEEEECCCCCCCCCHHHHHHH
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHCCCCHHHHHHH
FALISLASLALYGWRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGL
HHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGWGRLSVGAPYFNRATLPFALLMLVVIVLATFVSGKRAQLPALLAHAGVLLFAAGIVVS
HCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPER
CCCHHHEEEEECCCCEEEEEEEEEEEEEEEEEECCCCCCCHHEEEEHHHHHHHHCCCHHH
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMISGA
HHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHEEEEHHHCCEEEECCCHHHHHHH
LLSGWRGRKRDE
HHHCCCCCCCCC
>Mature Secondary Structure
MLTPLTAFAGVRLRWPAMMRLTCIGILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYS
CCCCCHHHCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCE
LLSWELKLAAVWGGHEGSLLLWVLLLSAWSALFAWHYRQQTDPLFPLTLTVLSLILAALL
EEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
LFVVGWSDPFVRIFPPAIEGRDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRG
HHHHCCCCCCEEEECCCCCCCCCCHHHHCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHC
EFDGTCARICWRWALPGWSALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSSTA
CCCCHHHHHHHHHCCCCHHHHHHHHHHHCHHHHEEECCCCEEEECCCCCCCCCHHHHHHH
LLHSLSLTRQRGIFRHWSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEHHHHHCCCCHHHHHHH
FALISLASLALYGWRARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGL
HHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGWGRLSVGAPYFNRATLPFALLMLVVIVLATFVSGKRAQLPALLAHAGVLLFAAGIVVS
HCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
SVSRQEISLNLQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPER
CCCHHHEEEEECCCCEEEEEEEEEEEEEEEEEECCCCCCCHHEEEEHHHHHHHHCCCHHH
RFYEARRQQMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMISGA
HHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCHHHEEEEHHHCCEEEECCCHHHHHHH
LLSGWRGRKRDE
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8057835; 8265357; 9278503 [H]