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The map label for this gene is iclR [H]

Identifier: 218692294

GI number: 218692294

Start: 4679654

End: 4680478

Strand: Reverse

Name: iclR [H]

Synonym: ECED1_4724

Alternate gene names: 218692294

Gene position: 4680478-4679654 (Counterclockwise)

Preceding gene: 218692299

Following gene: 218692289

Centisome position: 89.84

GC content: 54.91

Gene sequence:

>825_bases
ATGGTCGCACCCATTCCCGCAAAACGCGGCAGAAAACCCGCCGTTGCCACCGCACCAGCGACTGGACAGGTTCAGTCTTT
AACGCGTGGCCTGAAATTACTGGAGTGGATTGCCGAATCCAATGGCAGTGTGGCACTCACGGAACTGGCACAGCAAGCCG
GGTTACCCAATTCCACGACCCACCGCCTGCTAACCTCGATGCAACAGCAGGGCTTTGTCCGTCAGGTCGGCGAACTGGGT
CACTGGGCGATCGGCGCACACGCCTTTATGGTCGGCAGCAGCTTTCTCCAGAGCCGTAATTTGTTAGCGATTGTTCACCC
TATCCTGCGCAATCTAATGGAAGAGTCTGGCGAAACGGTCAATATGGCGGTGCTTGATCAAAGCGATCACGAAGCGATTA
TTATCGACCAGGTACAGTGTACTCACCTGATGCGAATGTCCGCGCCTATCGGCGGTAAATTGCCGATGCACGCTTCCGGT
GCGGGTAAAGCCTTTCTGGCCCAACTGAGCGAAGAACAGGTGACGAAGCTGCTGCACCGCAAAGGGTTACATGCCTATAC
CCACGCGACGCTGGTGTCTCCTGTGCATTTAAAAGAAGATCTCGCCCAAACGCGCAAACGGGGTTATTCATTTGACGATG
AGGAACATGCGCTGGGGCTGCGTTGCCTTGCAGCGTGTATTTTCGATGAGCACCGCGAACCGTTTGCCGCAATTTCAATT
TCCGGACCGATTTCACGTATTACCGATGACCGTGTGACCGAGTTTGGCGCGATGGTGATTAAAGCGGCGAAGGAAGTGAC
GCTGTCGTACGGTGGAATACGCTGA

Upstream 100 bases:

>100_bases
ATTTGTTCAACATTAACTCATCGGATCTGTTCAGCAACTATTGCATTCGCTGATAATAAAAATGAAAATGATTTCCACGA
TACAGAAAAAGGAGACTGTC

Downstream 100 bases:

>100_bases
CTTTTTCGCCTCTGATTTATACGAATCGAACGCTAAACCGCTGCCTGCGCCGATACGCATAAACATCTTCCACATGCCCT
TCACGTATGCGGTTTTGTAG

Product: transcriptional repressor IclR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTTHRLLTSMQQQGFVRQVGELG
HWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
SGPISRITDDRVTEFGAMVIKAAKEVTLSYGGIR

Sequences:

>Translated_274_residues
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTTHRLLTSMQQQGFVRQVGELG
HWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
SGPISRITDDRVTEFGAMVIKAAKEVTLSYGGIR
>Mature_274_residues
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTTHRLLTSMQQQGFVRQVGELG
HWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
SGPISRITDDRVTEFGAMVIKAAKEVTLSYGGIR

Specific function: Regulation of the glyoxylate bypass operon (aceBAK), which encodes isocitrate lyase, malate synthase as well as isocitrate dehydrogenase kinase/phosphorylase. Glyoxylate disrupts the interaction with the promoter by favoring the inactive dimeric form. Pyr

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI87082359, Length=274, Percent_Identity=98.9051094890511, Blast_Score=560, Evalue=1e-161,
Organism=Escherichia coli, GI1786715, Length=264, Percent_Identity=42.0454545454545, Blast_Score=220, Evalue=7e-59,
Organism=Escherichia coli, GI1788131, Length=248, Percent_Identity=29.4354838709677, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1786468, Length=232, Percent_Identity=30.6034482758621, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789999, Length=227, Percent_Identity=33.4801762114537, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790752, Length=249, Percent_Identity=26.9076305220884, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788581, Length=247, Percent_Identity=24.6963562753036, Blast_Score=85, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29724; Mature: 29724

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTT
CCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHH
HRLLTSMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEE
NMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASGAGKAFLAQLSEEQVTKLLHR
EEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
SGPISRITDDRVTEFGAMVIKAAKEVTLSYGGIR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSVALTELAQQAGLPNSTT
CCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHH
HRLLTSMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLLAIVHPILRNLMEESGETV
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEE
NMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASGAGKAFLAQLSEEQVTKLLHR
EEEEEECCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
KGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHALGLRCLAACIFDEHREPFAAISI
CCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
SGPISRITDDRVTEFGAMVIKAAKEVTLSYGGIR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185227; 1995431; 8265357; 9278503; 1995429 [H]