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The map label for this gene is citH [H]

Identifier: 218692252

GI number: 218692252

Start: 4620308

End: 4621624

Strand: Direct

Name: citH [H]

Synonym: ECED1_4671

Alternate gene names: 218692252

Gene position: 4620308-4621624 (Clockwise)

Preceding gene: 218692251

Following gene: 218692253

Centisome position: 88.69

GC content: 52.24

Gene sequence:

>1317_bases
ATGACGGCGATCGTAAATGAAACCGTGAGTGTGAAGAAAAAGCCCAGCGGCAGGCGGGTGATCTTTGCCTCGGCGTTTGG
CAATGCGCTCGAATTTTTTGATTTTGGCGTTTACAACTTTTTTGTGGTCTATATCAGTACGCTTTTCTTCCCACCCAGTG
CCGATCATAACGTCGCGCTGTTACTGGCATTTGCAACGTTTGGTGTGAGCTTTTTTATGCGTCCGCTCGGTGGGATTATC
GTTGGTGCCTGGGCCGATCGCTTCGGGCGTAAACCGGCGATGGTGTTCACTATTGCTCTGATGAGCCTCGGTACGCTGAT
GATCGGCATCGCACCGACGTATGAAACGGCGGGCTATTGGGGAACCGCAACGCTGGTGCTGGCGCGTCTAATTCAGGGTG
TTGCGGCAGGTGGTGAAGTCGGGGCTTCAATGTCGCTACTGGTGGAGTCAGCTCCGGCAAATCGTCGCGGCTTTTACAGC
AGTTGGTCACTGGCGACCCAAGGCCTGGCAACAACTTTTGGCGGTGTTGTCGCTTTAGGTTTAAGCGCATGGCTACCGTT
CGCCACAGGTTCAGAAACCGTAATGGCAGAATGGGGCTGGCGTGTGCCGTTTTTTATTGGCGTGTTATTAGCACCTATCG
GTTGCTGGTTGCGTCTGAGCCTGGAAAATGACGTACCTGAGCCAGTGCGCAATAAAAAAGCTGCTACCAGCGAAAGCGCG
TTTTCTCTGCTCCTGCAACATAAGGCGACTATCGTTAATGGCGTTTTACTCGCTATTGGTAGCACGGTAGCAACCTATAT
TTCCCTCTTTTATTACGGCACCTGGGCGGCGAAGTATCTGGCAATGCCTCAACACTATTCTCATGCAGCGATGTTACTGG
CGGGGGTAATTACGTTTGTGGGCGCGCTGTTGGTGGGGATGCTATGTGATTCGGTTGGGCGTAAAAAGCTGATTTTAATC
TCCCGTGTGATGGTGCTTCTCTGTAGCTGGCCGTCATTCTGGCTGTTGGTGAATTACCCAAGCCCTGGCATGTTGCTGAC
GGTGGTTTTCGTGATGGTCAGCTTTACCACGCTTGGCGGTGTGCCAGTGATGTTGTTGATCTCTGAACTGTTGCCGAAAC
GGATTCGGGCATTAGGTTTCGCGCTGGTTTATAGCATTGGCGTAGCAATCTTTGGCGGTTTTGCGCAATATTTCGCCACG
CAATCCATCGTGTTGCTGGATAGTCTGACGGCTCCGGCGTGGTATCTGGGTGGAGGCACGTTGCTGTCGATGCTGGCGTT
GTTGTACGTAAAAGAACCGGCCAAAGAATTGCAGTAA

Upstream 100 bases:

>100_bases
AAGCCTGGTCCCGGCAAGCAGCTACTGGGGCGCACTGGTTGAAGCCTGGCTGCAATAAATAAAACAACACACAACAACAC
TTTTTGGCAAGGAACATGGA

Downstream 100 bases:

>100_bases
ATATGTTTTATTGCGTTACCGTTCATTCACAACACTGGAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTA
CTTTTGGCAAGGATTCAGAC

Product: putative proton/ carboxylic acid permease

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVYISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGII
VGAWADRFGRKPAMVFTIALMSLGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLRLSLENDVPEPVRNKKAATSESA
FSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWAAKYLAMPQHYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILI
SRVMVLLCSWPSFWLLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFGGFAQYFAT
QSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ

Sequences:

>Translated_438_residues
MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVYISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGII
VGAWADRFGRKPAMVFTIALMSLGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLRLSLENDVPEPVRNKKAATSESA
FSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWAAKYLAMPQHYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILI
SRVMVLLCSWPSFWLLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFGGFAQYFAT
QSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ
>Mature_437_residues
TAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVYISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIV
GAWADRFGRKPAMVFTIALMSLGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYSS
WSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLRLSLENDVPEPVRNKKAATSESAF
SLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWAAKYLAMPQHYSHAAMLLAGVITFVGALLVGMLCDSVGRKKLILIS
RVMVLLCSWPSFWLLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFGGFAQYFATQ
SIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=439, Percent_Identity=32.5740318906606, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1790550, Length=447, Percent_Identity=30.2013422818792, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1788292, Length=426, Percent_Identity=27.9342723004695, Blast_Score=158, Evalue=7e-40,
Organism=Escherichia coli, GI1789941, Length=429, Percent_Identity=31.4685314685315, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI87082404, Length=386, Percent_Identity=25.9067357512953, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI87082231, Length=325, Percent_Identity=26.1538461538462, Blast_Score=86, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47109; Mature: 46978

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVYISTLFFPPSADHNVAL
CCCCCCCCHHCCCCCCCCEEEEECHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
LLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMSLGTLMIGIAPTYETAGYW
HHHHHHHHHHHHHHHHHHEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYSSWSLATQGLATTFGGVVALG
HHHHHHHHHHHHHHCCCCCCCCCHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLRLSLENDVPEPVRNKKAATSESA
HHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHEEEEECCCCCCCHHHCCCCCCHHHH
FSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWAAKYLAMPQHYSHAAMLLAGVITFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GALLVGMLCDSVGRKKLILISRVMVLLCSWPSFWLLVNYPSPGMLLTVVFVMVSFTTLGG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCC
VPVMLLISELLPKRIRALGFALVYSIGVAIFGGFAQYFATQSIVLLDSLTAPAWYLGGGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCEEECCCHH
LLSMLALLYVKEPAKELQ
HHHHHHHHHHHCHHHHCC
>Mature Secondary Structure 
TAIVNETVSVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVYISTLFFPPSADHNVAL
CCCCCCCHHCCCCCCCCEEEEECHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
LLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMSLGTLMIGIAPTYETAGYW
HHHHHHHHHHHHHHHHHHEEEEHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCC
GTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYSSWSLATQGLATTFGGVVALG
HHHHHHHHHHHHHHCCCCCCCCCHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLRLSLENDVPEPVRNKKAATSESA
HHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHEEEEECCCCCCCHHHCCCCCCHHHH
FSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWAAKYLAMPQHYSHAAMLLAGVITFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
GALLVGMLCDSVGRKKLILISRVMVLLCSWPSFWLLVNYPSPGMLLTVVFVMVSFTTLGG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCC
VPVMLLISELLPKRIRALGFALVYSIGVAIFGGFAQYFATQSIVLLDSLTAPAWYLGGGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEEECCCCCEEECCCHH
LLSMLALLYVKEPAKELQ
HHHHHHHHHHHCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2186908 [H]