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The map label for this gene is rspA [H]

Identifier: 218692247

GI number: 218692247

Start: 4613614

End: 4614888

Strand: Direct

Name: rspA [H]

Synonym: ECED1_4666

Alternate gene names: 218692247

Gene position: 4613614-4614888 (Clockwise)

Preceding gene: 218692246

Following gene: 218692248

Centisome position: 88.56

GC content: 49.41

Gene sequence:

>1275_bases
ATGGTAGTAGGCGTAAAATTAAGTTTGAAGTGTCTATTTACAATACAGGAGATTGCAATAATGAAAATTATTGCTGTGGA
TGTTTTTGTTACTTGTCCCGGGCGTAATTTCGTTACGCTTAAAATAACCACTGAAAGCGGACTTTGTGGATTAGGAGATG
CCACGTTAAATGGTCGCGAATTGTCGGTCGCTTCTTATCTGAAAGATCATCTTTGTCCTCAATTAATTGGCCGCGATGCC
AGCCGTATCGAAGATATCTGGCAATTCTTTTATAAAGGAGCTTACTGGCGACGTGGTCCCGTCACGATGTCGGCCATTTC
TGCCATTGATATGGCGTTGTGGGATATCAAAGCTAAAGCGGCAAATATGCCATTGTATCAACTGCTTGGTGGCGCTTCTC
GTGAAGGCGTGATGGTTTATTGCCACACCACCGGGCGGACGATTGATGAAGTCCTTGAAGACTATGCAAAGCACCAGCAG
ATGGGCTTTAAGGCGATTCGTGTGCAGTGTGGTGTACCGGGTATGCAAACCACCTATGGTCTGGCGAAGGGCAAAGGACT
GGCGTATGAACCAGCAACCAAAGGCCTGTGGCCAGAAGAGCAGTTGTGGTCTTCAGAAAAGTATCTCGATTTTACGCCGA
AGTTATTTGAAGCGGTGCGTAATAAGTTCGGTTTTAACGAACATCTGTTGCATGACATGCACCATCGCCTGACCCCCATC
GAAGCGGCGCGCTTTGGGAGGAGCATTGAGGATTACCGCCTGTTCTGGATGGAAGATCCGACGCCTGCCGAGAACCAGGA
ATGTTTTCGTCTGATTCGCCAGCATACCGTGACGCCGATTGCGGTCGGTGAAGTGTTTAACAGTATCTGGGACTGCAAGC
AGTTAATCGAAGAGCAGCTGATTGACTATATTCGCACGACGATTACCCATGCAGGCGGCATTACCGGGATGCGACGGATT
GCCGATTTTGCATCGCTTTATCAGGTACGTACCGGTTCGCATGGCCCTTCAGATTTATCGCCTGTTTGCCATGCGGCGGC
GCTGCATTTTGACCTCTGGGTGCCAAACTTTGGCGTTCAGGAGTACATGGGCTATTCCGAACAAATGCTTGAAGTCTTCC
CGCACAGCTGGCGCTTCGATGAAGGTTATATGCACCCAGGCGATGAACCGGGGCTGGGTATTTCTTTCGATGAGAAACTG
GCGGCGAAATATCCGTATGACCCGGCGTATCTCCCCGTCGCACGACTGGAAGATGGCACATTGTGGAACTGGTAA

Upstream 100 bases:

>100_bases
GGCTAATTATTCAGGTGGTTTTTGAGGGTGTTTTTTCTCCTCGGTAGAAATTTTTATTTTTAATGTGATCACGGTTGAAT
AATCACTTTTTCTCTCTTGT

Downstream 100 bases:

>100_bases
TAGCGGTTCGCTTAATTATTTTCTGAATATTCATCAAATCGATTAATTCCGCTCAAGGAGCTAAAAATGGACGTTGATGT
GTCAACCAGTGTTGCGGGTA

Product: putative enolase/dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MVVGVKLSLKCLFTIQEIAIMKIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVASYLKDHLCPQLIGRDA
SRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQ
MGFKAIRVQCGVPGMQTTYGLAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPI
EAARFGRSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTITHAGGITGMRRI
ADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKL
AAKYPYDPAYLPVARLEDGTLWNW

Sequences:

>Translated_424_residues
MVVGVKLSLKCLFTIQEIAIMKIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVASYLKDHLCPQLIGRDA
SRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQ
MGFKAIRVQCGVPGMQTTYGLAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPI
EAARFGRSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTITHAGGITGMRRI
ADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKL
AAKYPYDPAYLPVARLEDGTLWNW
>Mature_424_residues
MVVGVKLSLKCLFTIQEIAIMKIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVASYLKDHLCPQLIGRDA
SRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAANMPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQ
MGFKAIRVQCGVPGMQTTYGLAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPI
EAARFGRSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQLIDYIRTTITHAGGITGMRRI
ADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKL
AAKYPYDPAYLPVARLEDGTLWNW

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=404, Percent_Identity=91.3366336633663, Blast_Score=797, Evalue=0.0,
Organism=Escherichia coli, GI48994953, Length=414, Percent_Identity=29.4685990338164, Blast_Score=177, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 48041; Mature: 48041

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVGVKLSLKCLFTIQEIAIMKIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRE
CEEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCC
LSVASYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKA
HHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHH
ANMPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
CCCCHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHH
LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPI
HHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHH
EAARFGRSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQL
HHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHH
IDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEECCCCCHH
EYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLPVARLEDGT
HHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEECCCCC
LWNW
CCCC
>Mature Secondary Structure
MVVGVKLSLKCLFTIQEIAIMKIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRE
CEEEEEEHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCC
LSVASYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKA
HHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHH
ANMPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
CCCCHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHH
LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPI
HHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHH
EAARFGRSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQL
HHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHH
IDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNFGVQ
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHEEECCCCCHH
EYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLPVARLEDGT
HHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEECCCCC
LWNW
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]