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The map label for this gene is yfkN [H]

Identifier: 218692227

GI number: 218692227

Start: 4586204

End: 4587469

Strand: Direct

Name: yfkN [H]

Synonym: ECED1_4646

Alternate gene names: 218692227

Gene position: 4586204-4587469 (Clockwise)

Preceding gene: 218692224

Following gene: 218692228

Centisome position: 88.03

GC content: 49.13

Gene sequence:

>1266_bases
ATGAATACTATGCCGTTTGATGCTGTCACAATAGGCAACCATGAATTTGACCACGGCTGGGACAACACATTATTACAGTT
GAGCCAGGCAAAATTCCCTATTGTGCAGGGCAATATTTTTTATCAGAACAGCAGTAAATCATTCTGGGATAAGCCCTATA
CCATCATCGAAAAAGACGGCGTGAAAATTGGCGTTATAGGTTTGCACGGAGTATTTGCCTTCAATGATACGGTATCTGCT
GCCACGCGCGTGGGTATTGAAGCGCGCGATGAAATTAAATGGCTACAACGTTATATCGATGAACTCAAAGGCAAGGTTGA
TTTGACCGTCGCCCTGATCCACGAAGGTGTTCCAGCCCGCCAGTCCAGTATGGGAGGCACAGATGTGCGTCGCGCGTTGG
ATAAAGATATTCAGACGGCAAGTCAGGTGAAAGGGCTGGATATTTTGATCACCGGACATGCACATGTGGGTACGCCGGAA
CCGATTAAAGTCGGCAATACGCTCATCCTCTCCACTGACAGCGGCGGGATTGATGTGGGCAAACTGGTTCTCGACTACAA
AGAGAAGCCGCATAATTTTACGGTGAAAAACTTCGAGCTTAAAACCATTTACGCTGACGAGTGGAAGCCCGATCCGCAAA
CGAAACAGGTGATCGACGGCTGGAATAAAAAGCTTGATGAAGTGGTGCAACAAACGGTGGCGCAATCGCCGGTTGAATTA
AAACGTGCCTATGGTGAATCGGCTTCCCTCGGGAACCTGGCGGCTGACGCTTTACTGGCAGCAGCGGGTAAAAATACCCA
ACTGGCGTTAACAAACTCTGGTGGTATCCGCAATGAGATCCCCGCAGGGGCGATCACGATGGGCGGCGTCATCAGTACCT
TCCCGTTCCCCAACGAACTGGTGACGATGGAACTCACGGGTAAACAATTACGCAGTCTGATGGAACACGGCGCAAGTTTA
AGCAATGGCGTTTTACAGGTATCGAAAGGCCTGGAAATGAAGTACGACAGCAGCAAGCCGGTTGGTCAGCGGGTAATCAC
ACTGACTCTGAATGGCAAACCCATTGAAGATGCGACGGTTTATCACATTGCTACTCAGAGTTTCCTTGCTGATGGTGGAG
ATGGTTTTACCGCCTTTACCGAAGGGAAAGCGCGTAACACAACGGGCGGTTATTACGTTTATCACGCGGTGGTTGATTAC
TTCAAAGCGGGCAACACCATTACGGATGAACAGCTCAACGGTATGCGCGTGAAGGATATCAAATAA

Upstream 100 bases:

>100_bases
CAAAATAAAAGGTGGCTTTATTTTTGGCTTTCTCTTGTTTTACTAACGTGGATATATTGGCAAAGCCACCAATGTCGCGT
TTTCCGGTATTATCGATATT

Downstream 100 bases:

>100_bases
AAGTGCGCACTGAATTGACTCAACTGTTTGTTACCCGTTGATTGCGCAGATCCTGTATTCGGCCTACGCGAGGGCGCAGG
CCGAATCAGATGCCTTAACA

Product: putative phosphoesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNTMPFDAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIGVIGLHGVFAFNDTVSA
ATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPE
PIKVGNTLILSTDSGGIDVGKLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPVEL
KRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNELVTMELTGKQLRSLMEHGASL
SNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDY
FKAGNTITDEQLNGMRVKDIK

Sequences:

>Translated_421_residues
MNTMPFDAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIGVIGLHGVFAFNDTVSA
ATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPE
PIKVGNTLILSTDSGGIDVGKLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPVEL
KRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNELVTMELTGKQLRSLMEHGASL
SNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDY
FKAGNTITDEQLNGMRVKDIK
>Mature_421_residues
MNTMPFDAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDGVKIGVIGLHGVFAFNDTVSA
ATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPE
PIKVGNTLILSTDSGGIDVGKLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPVEL
KRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNELVTMELTGKQLRSLMEHGASL
SNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATVYHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDY
FKAGNTITDEQLNGMRVKDIK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=438, Percent_Identity=25.7990867579909, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI1786687, Length=428, Percent_Identity=24.7663551401869, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1790658, Length=461, Percent_Identity=22.9934924078091, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI221329836, Length=461, Percent_Identity=26.4642082429501, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24641187, Length=435, Percent_Identity=27.1264367816092, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI19922446, Length=441, Percent_Identity=25.6235827664399, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24654424, Length=441, Percent_Identity=25.6235827664399, Blast_Score=108, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24652512, Length=411, Percent_Identity=25.3041362530414, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI161076508, Length=411, Percent_Identity=25.3041362530414, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19921980, Length=411, Percent_Identity=25.3041362530414, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI19922444, Length=473, Percent_Identity=24.7357293868922, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI28573524, Length=463, Percent_Identity=22.6781857451404, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 45814; Mature: 45814

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00786 5_NUCLEOTIDASE_2 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTMPFDAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDG
CCCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCEEECEEEEECCCCCCCCCCCEEEECCC
VKIGVIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPAR
CEEEEEEEEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCC
QSSMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEECCCEEEEEECCCCCCHH
KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPVEL
HEEEECCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
KRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNEL
HHHCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCE
VTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATV
EEEEECHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCCCCCCEEEEEEECCCCCCCCEE
YHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQLNGMRVKDI
EEEEHHHHHCCCCCCCEEEECCCCCCCCCCEEHHHHHHHHHHCCCEECHHCCCCEEEECC
K
C
>Mature Secondary Structure
MNTMPFDAVTIGNHEFDHGWDNTLLQLSQAKFPIVQGNIFYQNSSKSFWDKPYTIIEKDG
CCCCCCCEEEECCCCCCCCCCHHHHHHHCCCCCEEECEEEEECCCCCCCCCCCEEEECCC
VKIGVIGLHGVFAFNDTVSAATRVGIEARDEIKWLQRYIDELKGKVDLTVALIHEGVPAR
CEEEEEEEEEEEEECCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEECCCCCC
QSSMGGTDVRRALDKDIQTASQVKGLDILITGHAHVGTPEPIKVGNTLILSTDSGGIDVG
CCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCEECCCEEEEEECCCCCCHH
KLVLDYKEKPHNFTVKNFELKTIYADEWKPDPQTKQVIDGWNKKLDEVVQQTVAQSPVEL
HEEEECCCCCCCEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHH
KRAYGESASLGNLAADALLAAAGKNTQLALTNSGGIRNEIPAGAITMGGVISTFPFPNEL
HHHCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCE
VTMELTGKQLRSLMEHGASLSNGVLQVSKGLEMKYDSSKPVGQRVITLTLNGKPIEDATV
EEEEECHHHHHHHHHCCCCCCCCEEEECCCCEEEECCCCCCCCEEEEEEECCCCCCCCEE
YHIATQSFLADGGDGFTAFTEGKARNTTGGYYVYHAVVDYFKAGNTITDEQLNGMRVKDI
EEEEHHHHHCCCCCCCEEEECCCCCCCCCCEEHHHHHHHHHHCCCEECHHCCCCEEEECC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]