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The map label for this gene is aes [H]

Identifier: 218692174

GI number: 218692174

Start: 4532597

End: 4533580

Strand: Reverse

Name: aes [H]

Synonym: ECED1_4589

Alternate gene names: 218692174

Gene position: 4533580-4532597 (Counterclockwise)

Preceding gene: 218692179

Following gene: 218692167

Centisome position: 87.02

GC content: 51.12

Gene sequence:

>984_bases
GTGAATTTCCCTGTATCCATCCAGCAAAAACGGGTGTATAAAACTGTACATTCATACAGCAAAATGAGTTCTACTATGGC
ACTGGAAAAAGGAATTGCAGAGTTAGTCGAAGAGTTTATCGCTGCGGGTCGCCCCTCATCGCGCCAACAAAGTATTACGC
AGCGCAGAGAGGGCTATATCGCCAGCGCTGTCCTTGCGGGTGAGACAGAAACGCGGGTAGATATTCAGACTATCGAACTT
GAAGGGATGACCTTACGAATAGTGTCACCGCTGAATGCCCCGACATTACTCCCGACGATAATTTATTACTACGGTGGCTG
TTTTGTTAGCGGCGGGTTCGCAACTCACGACAACCAGCTGCGCCAGTTAGCCTATTACGGGCAATGTCGGATTATTGCCG
TCCAGTATCGGCTGGCACCGGAACATACTTTCCCCGCCGCCCATGATGATGCACAACGAGGTGCAGAATTAGTCCGGCAA
CACGCCGAACGACTCGGCGTTGATAAACAACGAATAACTCTTGCCGGTGACAGTGCGGGAGGGCATCTGGCGCTGGTAAC
TGCTTTGCGATTGAAGCGTGCTGGCGAGTGGCAACCAGCACAACTCATCTTAATTTATCCTATGCTTGATGCGACTGCGC
ATTTCGAAAGCTACATACGCAATGGTCACGATTACATCATCACACGCGACACTTTACTGAGCGGCTTTGAGATGTACCTG
CCAGGCATTGAACGCAGGCATCCTGAAGCCAGCCCTATTTGGCGCAATGATTTCAATGGCTTGCCACCTGTACATATCAT
TACCGCTGAATACGACCCACTTTGCGATGAAGGTGAAGCGTTGTATCACCGCATGACAGGGCAAGGAGTGCAATGCACCT
GCCAACGCTACCTTGGCGTTATCCACGGATTTTTTCAGTTAGGTGGTATCAGTGAGGCTGCGCGATCGGCGCTACGGGAT
GTTGCCTGGAGAGCGGGAAGGTAA

Upstream 100 bases:

>100_bases
CCATTGAAACCACTAGCTTACTGATAGTGCTCCAGCAAAACCACGTCTCTGCCGGGTTTGTCTTTACATTTGCGAGGCGC
TTTCCAGATTTTTCACGCCC

Downstream 100 bases:

>100_bases
CTCCGGACCAGGGCCCAGAAAAGGTTTTCAGATTGAAAACCTTTTCAGTCAAGTAAAACGCCCCTACTCTTCTTCGTTTC
CGCCCTCTTCATACGGACCA

Product: putative lipase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNFPVSIQQKRVYKTVHSYSKMSSTMALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGETETRVDIQTIEL
EGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYGQCRIIAVQYRLAPEHTFPAAHDDAQRGAELVRQ
HAERLGVDKQRITLAGDSAGGHLALVTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYL
PGIERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVIHGFFQLGGISEAARSALRD
VAWRAGR

Sequences:

>Translated_327_residues
MNFPVSIQQKRVYKTVHSYSKMSSTMALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGETETRVDIQTIEL
EGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYGQCRIIAVQYRLAPEHTFPAAHDDAQRGAELVRQ
HAERLGVDKQRITLAGDSAGGHLALVTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYL
PGIERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVIHGFFQLGGISEAARSALRD
VAWRAGR
>Mature_327_residues
MNFPVSIQQKRVYKTVHSYSKMSSTMALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGETETRVDIQTIEL
EGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYGQCRIIAVQYRLAPEHTFPAAHDDAQRGAELVRQ
HAERLGVDKQRITLAGDSAGGHLALVTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYL
PGIERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVIHGFFQLGGISEAARSALRD
VAWRAGR

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=266, Percent_Identity=27.4436090225564, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI21328446, Length=118, Percent_Identity=35.5932203389831, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786682, Length=285, Percent_Identity=30.8771929824561, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17567059, Length=265, Percent_Identity=26.7924528301887, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71996133, Length=170, Percent_Identity=30.5882352941176, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI72001146, Length=113, Percent_Identity=32.7433628318584, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17540028, Length=148, Percent_Identity=30.4054054054054, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115533410, Length=133, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI115533412, Length=133, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24656084, Length=110, Percent_Identity=35.4545454545455, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24656076, Length=110, Percent_Identity=35.4545454545455, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20130169, Length=110, Percent_Identity=35.4545454545455, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 36550; Mature: 36550

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFPVSIQQKRVYKTVHSYSKMSSTMALEKGIAELVEEFIAAGRPSSRQQSITQRREGYI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCE
ASAVLAGETETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQL
EEEEEECCCCCEEEEEEEEECCEEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCHHHH
RQLAYYGQCRIIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAG
HHHHHHCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
GHLALVTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYL
CHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHC
PGIERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGV
CCHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHH
IHGFFQLGGISEAARSALRDVAWRAGR
HHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFPVSIQQKRVYKTVHSYSKMSSTMALEKGIAELVEEFIAAGRPSSRQQSITQRREGYI
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCE
ASAVLAGETETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQL
EEEEEECCCCCEEEEEEEEECCEEEEEECCCCCCHHHHHHHHHHCCCEEECCCCCCHHHH
RQLAYYGQCRIIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAG
HHHHHHCCEEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
GHLALVTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYL
CHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHC
PGIERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGV
CCHHHCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHHHHHHH
IHGFFQLGGISEAARSALRDVAWRAGR
HHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA