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The map label for this gene is fadA [H]

Identifier: 218692137

GI number: 218692137

Start: 4484484

End: 4485647

Strand: Reverse

Name: fadA [H]

Synonym: ECED1_4547

Alternate gene names: 218692137

Gene position: 4485647-4484484 (Counterclockwise)

Preceding gene: 218692138

Following gene: 218692134

Centisome position: 86.1

GC content: 57.73

Gene sequence:

>1164_bases
ATGGAACAGGTTGTCATTGTCGATGCAATTCGCACCCCGATGGGCCGTTCGAAGGGCGGTGCTTTTCGTAACGTGCGTGC
GGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCAGCGGCCCTCGACGATATTT
ACTGGGGTTGTGTGCAGCAAACACTGGAGCAGGGTTTTAACATCGCCCGTAACGCGGCACTGTTGGCTGAAGTGCCGCAC
TCTGTCCCGGCGGTTACCGTTAATCGCTTGTGTGGTTCATCCATGCAGGCACTGCATGACGCAGCACGAATGATCATGAC
CGGCGATGCGCAGGCATGTCTGGTTGGCGGCGTGGAGCATATGGGCCATGTGCCAATGAGTCACGGCGTCGATTTTCACC
CCGGCCTTAGCCGTAATGTCGCCAAAGCGGCGGGCATGATGGGCTTAACGGCAGAAATGCTGGCGCGTATGCATGGTATC
AGCCGTGAAATGCAGGATGCCTTTGCCGCGCGGTCACACGCCCGCGCGTGGGCCGCCACGCAGTCAGGCGCATTTAAAAA
TGAAATCATCCCGACCGGTGGTCACGATGCCGACGGCGTATTGAAGCAGTTTAATTACGACGAAGTGATTCGCCCGGAAA
CCACCGTGGAAGCCCTCGCCACACTGCGTCCGGCGTTTGATCCCGTCAGCGGTACGGTAACGGCGGGTACATCTTCTGCG
CTTTCCGATGGCGCAGCTGCCATGCTGGTGATGAGTGAAAGCCGCGCCCGTGAATTAGGTCTTAAGCCGCGCGCCCGCGT
GCGTTCGATGGCGGTTGTGGGTTGTGACCCATCTATTATGGGTTATGGTCCGGTTCCGGCCTCGAAGCTGGCGCTGAAAA
AAGCGGGGCTTTCTGCCAGCGATATCGGCGTATTCGAGATGAACGAAGCCTTTGCCGCGCAGATCCTGCCGTGTATTAAA
GATCTCGGGCTAATAGAGCAGATTGACGAGAAGATCAACCTCAACGGTGGCGCGATCGCGCTGGGTCATCCGCTGGGTTG
TTCCGGTGCGCGTATCAGCACCACGCTGCTGAATCTGATGGAACACAAAGACGTCCAGTTTGGTCTGGCGACGATGTGTA
TCGGTCTGGGTCAGGGCATTGCAACGGTGTTTGAGCGGGTTTAA

Upstream 100 bases:

>100_bases
GGAAGGTCTGCGTAATAAAGCACGTCATAACGAACCGTACTATCCCCCGGTTGAGCCAGCCCGTCCGGTTGGCGACCTGA
AAACGGCTTAAGGAGTCACA

Downstream 100 bases:

>100_bases
CCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACGGTTCAGAACTTTTGTAGGCCCGATAAGACGCGGCAGCGT
CGCATCGGGCACTGTATGCC

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
LSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIK
DLGLIEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV

Sequences:

>Translated_387_residues
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
LSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIK
DLGLIEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV
>Mature_387_residues
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
LSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIK
DLGLIEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=42.9292929292929, Blast_Score=255, Evalue=4e-68,
Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=38.4810126582278, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI148539872, Length=400, Percent_Identity=38.75, Blast_Score=231, Evalue=9e-61,
Organism=Homo sapiens, GI4504327, Length=429, Percent_Identity=34.032634032634, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI4557237, Length=402, Percent_Identity=34.5771144278607, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI194353979, Length=393, Percent_Identity=31.5521628498728, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI48994986, Length=387, Percent_Identity=98.7080103359173, Blast_Score=778, Evalue=0.0,
Organism=Escherichia coli, GI1787663, Length=405, Percent_Identity=45.1851851851852, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI1788554, Length=405, Percent_Identity=43.4567901234568, Blast_Score=270, Evalue=2e-73,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=41.3965087281795, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI1788683, Length=418, Percent_Identity=35.4066985645933, Blast_Score=191, Evalue=8e-50,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=41.3965087281795, Blast_Score=264, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=33.953488372093, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17535921, Length=403, Percent_Identity=33.7468982630273, Blast_Score=190, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI25147385, Length=395, Percent_Identity=31.6455696202532, Blast_Score=161, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17535917, Length=388, Percent_Identity=27.5773195876289, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6322031, Length=391, Percent_Identity=39.8976982097187, Blast_Score=232, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6325229, Length=408, Percent_Identity=34.8039215686275, Blast_Score=189, Evalue=6e-49,
Organism=Drosophila melanogaster, GI24655093, Length=402, Percent_Identity=40.2985074626866, Blast_Score=255, Evalue=4e-68,
Organism=Drosophila melanogaster, GI17648125, Length=402, Percent_Identity=39.3034825870647, Blast_Score=234, Evalue=9e-62,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=34.032634032634, Blast_Score=201, Evalue=8e-52,
Organism=Drosophila melanogaster, GI24640423, Length=406, Percent_Identity=31.7733990147783, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40806; Mature: 40806

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQ
CCCEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEH
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHCHHH
MGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGISREMQDAFAARSHARAWAAT
HCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHH
LSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSAS
HCCCCEEEEEECHHHHHHCCCCCHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHCCCCCC
DIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLM
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
EHKDVQFGLATMCIGLGQGIATVFERV
HHCCHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQ
CCCEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEH
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCCHHHHHCHHH
MGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGISREMQDAFAARSHARAWAAT
HCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVSGTVTAGTSSA
CCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHH
LSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSAS
HCCCCEEEEEECHHHHHHCCCCCHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHCCCCCC
DIGVFEMNEAFAAQILPCIKDLGLIEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLM
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
EHKDVQFGLATMCIGLGQGIATVFERV
HHCCHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA