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The map label for this gene is rffA [H]

Identifier: 218692070

GI number: 218692070

Start: 4411074

End: 4412204

Strand: Direct

Name: rffA [H]

Synonym: ECED1_4476

Alternate gene names: 218692070

Gene position: 4411074-4412204 (Clockwise)

Preceding gene: 218692069

Following gene: 218692071

Centisome position: 84.67

GC content: 54.64

Gene sequence:

>1131_bases
ATGATTCCATTTAACGCACCGCCGGTGGTGGGAACCGAACTCGACTATATGCAGTCGGCAATGGGTAGCGGCAAACTGTG
CGGCGATGGTGGTTTTACCCGTCGCTGCCAGCAGTGGCTGGAGCAACGTTTTGGCAGCGCCAAAGTGTTACTGACGCCAT
CCTGTACCGCTTCGCTGGAGATGGCGGCGCTGCTGCTCGATATCCAGCCTGGCGATGAAGTGATCATGCCGAGCTATACC
TTTGTCTCCACCGCCAATGCCTTTGTGCTGCGTGGCGCGAAAATCGTCTTTGTGGATGTTCGCCCGGACACCATGAACAT
CGACGAAACGCTGATTGAAGCGGCGATCACCGACAAAACGCGCGTTATCGTGCCGGTCCATTACGCGGGTGTGGCCTGCG
AAATGGACACCATTATGGCGTTGGCGAAGAAGCATAATCTGTTTGTGGTGGAAGATGCCGCTCAGGGCGTGATGTCCACT
TACAAAGGGCGTGCGCTGGGAACCATTGGTCATATTGGCTGCTTTAGCTTCCATGAAACCAAAAACTACACGGCGGGTGG
TGAAGGCGGCGCGACGCTGATTAACGATAAAGCGTTGATCGAACGAGCCGAGATCATCCGTGAAAAAGGCACAAACCGCA
GCCAGTTCTTCCGTGGTCAGGTCGATAAATATACCTGGCGCGATATTGGCTCCAGCTATTTGATGTCCGATCTGCAAGCT
GCGTACCTGTGGGCGCAACTGGAAGCTGCGGATCGTATCAACCAGCAACGTCTGGCGCTGTGGCAAAACTACTACGATGC
GCTGGCGCCTCTGGCAAAAAATGGGCGTATCGAGCTGCCGTCGATTCCCGATGGCTGCGTGCAGAACGCGCATATGTTCT
ACATTAAACTGCGGGATATTGATGACCGGAGCGCATTGATTAACTTTCTGAAAGAAGCGGAAATCATGGCGGTGTTCCAT
TACATTCCGCTGCACGGTTGCCCTGCGGGGGAACGCTTTGGTGAGTTCCACGGTGAAGATCGCTACACCACCAAAGAGAG
CGAGCGCCTGCTGCGCCTGCCGTTGTTCTACAACCTGTCGCCCGTCAATCAGCGTACGGTAATTGCGACCTTGTTGAACT
ACTTCTCCTGA

Upstream 100 bases:

>100_bases
CGGGTGGCGACTCAGATGGGCAACACCGCCGCGCTTAAACGATACATACAAAGTGGTGCGAATGTAGAAAGCACCGCGTA
CTGGTTATACAGGTGATCAC

Downstream 100 bases:

>100_bases
TATGTCGTTGGCAAAAGCGTCCTTGTGGACGGCGGCCAGTACACTGGTCAAGATTGGTGCCGGGTTACTGGTCGGTAAGT
TGCTGGCAGTGTCATTTGGT

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRFGSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYT
FVSTANAFVLRGAKIVFVDVRPDTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
AYLWAQLEAADRINQQRLALWQNYYDALAPLAKNGRIELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFH
YIPLHGCPAGERFGEFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS

Sequences:

>Translated_376_residues
MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRFGSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYT
FVSTANAFVLRGAKIVFVDVRPDTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
AYLWAQLEAADRINQQRLALWQNYYDALAPLAKNGRIELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFH
YIPLHGCPAGERFGEFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS
>Mature_376_residues
MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRFGSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYT
FVSTANAFVLRGAKIVFVDVRPDTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
AYLWAQLEAADRINQQRLALWQNYYDALAPLAKNGRIELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFH
YIPLHGCPAGERFGEFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=376, Percent_Identity=99.468085106383, Blast_Score=782, Evalue=0.0,
Organism=Escherichia coli, GI145693159, Length=379, Percent_Identity=31.9261213720317, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 41964; Mature: 41964

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRFGSAKVLLTPSCTASLE
CCCCCCCCCCCCCHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHE
MAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRPDTMNIDETLIEAAITDKT
EEEEEEEECCCCCEEECCCHHHHCCCEEEEECCEEEEEEECCCCCCHHHHHHHHHHCCCC
RVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMSTYKGRALGTIGHIGCFSFHET
EEEEEEEECCEEEHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCEEECHHHHEEEEEECC
KNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
CCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHCCHHHHHHHHHH
AYLWAQLEAADRINQQRLALWQNYYDALAPLAKNGRIELPSIPDGCVQNAHMFYIKLRDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHCCCEEEEEEEECC
DDRSALINFLKEAEIMAVFHYIPLHGCPAGERFGEFHGEDRYTTKESERLLRLPLFYNLS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCHHHHEEECCEEECCC
PVNQRTVIATLLNYFS
CCCHHHHHHHHHHHCC
>Mature Secondary Structure
MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRFGSAKVLLTPSCTASLE
CCCCCCCCCCCCCHHHHHHHCCCCCEECCCCHHHHHHHHHHHHCCCEEEEECCCCCHHHE
MAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRPDTMNIDETLIEAAITDKT
EEEEEEEECCCCCEEECCCHHHHCCCEEEEECCEEEEEEECCCCCCHHHHHHHHHHCCCC
RVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMSTYKGRALGTIGHIGCFSFHET
EEEEEEEECCEEEHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCEEECHHHHEEEEEECC
KNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
CCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHCCHHHHHHHHHH
AYLWAQLEAADRINQQRLALWQNYYDALAPLAKNGRIELPSIPDGCVQNAHMFYIKLRDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCHHHHCCCEEEEEEEECC
DDRSALINFLKEAEIMAVFHYIPLHGCPAGERFGEFHGEDRYTTKESERLLRLPLFYNLS
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCHHHHEEECCEEECCC
PVNQRTVIATLLNYFS
CCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]