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The map label for this gene is yibK

Identifier: 218691893

GI number: 218691893

Start: 4218352

End: 4218825

Strand: Direct

Name: yibK

Synonym: ECED1_4292

Alternate gene names: 218691893

Gene position: 4218352-4218825 (Clockwise)

Preceding gene: 218691892

Following gene: 218691899

Centisome position: 80.97

GC content: 54.85

Gene sequence:

>474_bases
ATGCTAAACATCGTACTTTACGAACCAGAAATTCCGCCAAATACTGGCAACATCATCCGTCTTTGCGCTAATACCGGCTT
TCGTCTGCATATCATCGAACCGATGGGATTTGCCTGGGACGATAAGCGCCTGCGCCGCGCGGGGCTGGACTATCACGAGT
TTACCGCCGTTACGCGTCATCATGACTATCGCGCGTTTCTCGAAGCAGAAAATCCCCAGCGTCTGTTTGCCCTCACCACG
AAAGGTACGCCTGCTCACAGCGCCGTAAGCTATCAGGATGGCGATTATCTGATGTTCGGCCCGGAAACACGCGGCCTGCC
AGCGAGCATTCTTGATGCCCTGCCCGCTGAACAAAAAATTCGTATTCCAATGGTGCCGGACAGCCGCAGCATGAACCTGT
CCAATGCGGTGTCGGTGGTGGTGTATGAAGCCTGGCGCCAGCTGGGGTATCCGGGAGCGGTGTTGAGAGATTAA

Upstream 100 bases:

>100_bases
GTAAAGAGTTGCCTGCGGCACTGGCTCCGATGGCGAAAGGGAATGCAGCTTAAGGGTTAGACGAATATCTGCTATCCTGC
CCCCGCACTAAGGGGGCAGT

Downstream 100 bases:

>100_bases
GATGCGGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCACGATCACAGGACATCAGATCCCATCACCATACTCAAACG
TATGGTTAATACCGTTGAAG

Product: putative tRNA/rRNA methyltransferase YibK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTT
KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD

Sequences:

>Translated_157_residues
MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTT
KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD
>Mature_157_residues
MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTT
KGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily

Homologues:

Organism=Escherichia coli, GI1790034, Length=157, Percent_Identity=100, Blast_Score=327, Evalue=2e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRML_ECOL6 (P0AGJ8)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_756290.1
- ProteinModelPortal:   P0AGJ8
- SMR:   P0AGJ8
- EnsemblBacteria:   EBESCT00000042209
- GeneID:   1038081
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c4428
- GeneTree:   EBGT00050000011381
- HOGENOM:   HBG309728
- OMA:   EAWRQNG
- ProtClustDB:   PRK10358
- GO:   GO:0005737
- HAMAP:   MF_01885
- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537
- PIRSF:   PIRSF029256
- TIGRFAMs:   TIGR00185

Pfam domain/function: PF00588 SpoU_methylase

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17726; Mature: 17726

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: BINDING 78-78 BINDING 100-100 BINDING 122-122 BINDING 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRH
CEEEEEECCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHEECCC
HDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKI
CCEEEEEECCCCCEEEEEEECCCCCCCEEEECCCCEEEECCCCCCCCHHHHHCCCCCCEE
RIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRLRRAGLDYHEFTAVTRH
CEEEEEECCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCHHHHHHHCCCHHHHHEECCC
HDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPETRGLPASILDALPAEQKI
CCEEEEEECCCCCEEEEEEECCCCCCCEEEECCCCEEEECCCCCCCCHHHHHCCCCCCEE
RIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157