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The map label for this gene is treF [H]

Identifier: 218691803

GI number: 218691803

Start: 4106077

End: 4107726

Strand: Direct

Name: treF [H]

Synonym: ECED1_4197

Alternate gene names: 218691803

Gene position: 4106077-4107726 (Clockwise)

Preceding gene: 218691798

Following gene: 218691805

Centisome position: 78.82

GC content: 54.36

Gene sequence:

>1650_bases
ATGCTCAATCAGAAAATTCAAAACCCTAATCCAGACGAACTGATGATCGAGGTCGATCTCTGCTATGAGCTGGACCCGTA
TGAATTAAAACTGGATGAGATGATCGAGGCAGAACCGGAACCCGAGATGATTGAAGGGCTGCCCGCCTCTGATGCGCTGA
CGCCTGCCGATCGCTATCTCGAACTGTTCGAGCATGTTCAGTCGGAGAAAATTTTCCCCGACAGTAAAACCTTTCCCGAC
TGCGCACCCAAAATGGACCCGCTGGATATTTTAATCCGCTACCGTAAAGTGCGCCGTCATCGTGATTTTGACTTGCGCAA
GTTTGTTGAAAACCACTTCTGGCTGCCGGAGGTCTACTCCAGCGAGTATGTATCGGACCCGCAAAATTCCCTGAAAGAGC
ATATCGACCAGCTGTGGCCGGTGCTAACCCGTGAACCACAGGATCACATTCCGTGGTCTTCTCTGCTGGCGCTGCCGCAG
TCATATATTGTCCCGGGCGGCCGTTTTAGCGAAACCTACTATTGGGATTCCTATTTCACCATGCTGGGGCTGGCGGAAAG
TGGTCGGGAAGATTTGCTGAAATGCATGGCCGATAACTTCGCCTGGATGATCGAAAACTACGGCCACATCCCCAACGGCA
ACCGCACCTATTATTTGAGCCGCTCGCAACCACCGGTTTTTGCGCTGATGGTGGAGTTGTTTGAAGAAGATGGTGTACGC
GGCGCGCGCCGCTATCTCGACCACCTGAAAATGGAATATGCCTTCTGGATGGACGGTGCAGAATCGTTGATCCCTAATCA
GGCCTATCGCCATGTTGTGCGGATGCCGGACGGCTCGCTGCTCAACCGTTACTGGGACGATCGCGACACGCCGCGTGACG
AATCCTGGCTTGAGGACGTTGAAACCGCGAAACATTCTGGTCGCCCGCCTAACGAGGTGTACCGCGATTTACGTGCGGGG
GCGGCCTCCGGTTGGGATTACTCTTCCCGTTGGCTGCGTGATACTGGTCGTCTGGCGAGCATTCGTACCACCCAGTTCAT
CCCCATCGATCTGAATGCCTTCCTGTTTAAACTGGAGAGCGCCATCGCCAACATCTCGGCGCTGAAAGGCGAGAAAGAGA
CAGAAGCACTGTTCCGCCAGAAGGCCAGTGCCCGTCGCGATGCGGTAAACCGTTACCTCTGGGATGATGAAAACGGCATC
TACCGCGATTACGACTGGCGACGCGAGCAACTGGCGCTGTTTTCCGCTGCCGCCATTGTGCCGCTCTATGTCGGTATGGC
GAACCATGAACAGGCCGATCGTCTGGCAAACGCCGTGCGCAGCCGGTTACTGACACCTGGCGGGATTCTGGCAAGCGAGT
ACGAAACCGGTGAACAGTGGGATAAACCCAACGGCTGGGCACCGTTACAATGGATGGCAATTCAGGGATTTAAGATGTAC
GGCGATGACCTTCTGGGTGATGAAATCGCGCGCAACTGGCTGAAGACGGTGAATCAGTTCTATCTGGAACAGCACAAACT
GATCGAGAAATACCATATTGCCGATGGTGTTCCTCGCGAAGGCGGCGGTGGCGAGTATCCGTTGCAGGATGGGTTTGGCT
GGACTAACGGTGTGGTACGCCGTTTAATTGGTTTGTACGGCGAACCATAA

Upstream 100 bases:

>100_bases
CGTGATCCACCGCACGCTTTGTCGCCCACCAGGCGGAGCGAATGACTACCCTTAAAGAAAAGCCCGATAATTAGCGACGA
ATTTCGGAGGTCGGATCCTT

Downstream 100 bases:

>100_bases
TATTTTTACAGCCAGCCGCTAACTTCCTGCTGGCTGTAAAATTATCCTCTTCAGGAGGAGATATTTAACATCATTGCCGC
CTGGGTGCGATTTTTGACTT

Product: trehalase

Products: NA

Alternate protein names: Alpha,alpha-trehalase; Alpha,alpha-trehalose glucohydrolase [H]

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSEKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDLLGDEIARNWLKTVNQFYLEQHKLIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP

Sequences:

>Translated_549_residues
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSEKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDLLGDEIARNWLKTVNQFYLEQHKLIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP
>Mature_549_residues
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYLELFEHVQSEKIFPDSKTFPD
CAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAG
AASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGI
YRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
GDDLLGDEIARNWLKTVNQFYLEQHKLIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP

Specific function: Hydrolyzes trehalose to glucose. Could be involved, in cells returning to low osmolarity conditions, in the utilization of the accumulated cytoplasmic trehalose, which was synthesized in response to high osmolarity [H]

COG id: COG1626

COG function: function code G; Neutral trehalase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 37 family [H]

Homologues:

Organism=Homo sapiens, GI116284412, Length=525, Percent_Identity=34.0952380952381, Blast_Score=254, Evalue=1e-67,
Organism=Escherichia coli, GI1789936, Length=549, Percent_Identity=99.6357012750455, Blast_Score=1127, Evalue=0.0,
Organism=Escherichia coli, GI1787447, Length=487, Percent_Identity=50.9240246406571, Blast_Score=499, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI17542196, Length=527, Percent_Identity=31.6888045540797, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI25141398, Length=529, Percent_Identity=31.1909262759924, Blast_Score=237, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17565078, Length=510, Percent_Identity=31.3725490196078, Blast_Score=233, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI25148109, Length=546, Percent_Identity=30.03663003663, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI71987755, Length=570, Percent_Identity=28.0701754385965, Blast_Score=192, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6320204, Length=473, Percent_Identity=27.6955602536998, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319473, Length=481, Percent_Identity=28.6902286902287, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24656680, Length=550, Percent_Identity=31.0909090909091, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24656675, Length=550, Percent_Identity=31.0909090909091, Blast_Score=229, Evalue=3e-60,
Organism=Drosophila melanogaster, GI24656661, Length=525, Percent_Identity=31.4285714285714, Blast_Score=228, Evalue=1e-59,
Organism=Drosophila melanogaster, GI17933716, Length=525, Percent_Identity=31.4285714285714, Blast_Score=228, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24656670, Length=525, Percent_Identity=31.4285714285714, Blast_Score=228, Evalue=1e-59,
Organism=Drosophila melanogaster, GI24656685, Length=486, Percent_Identity=31.8930041152263, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI22024178, Length=533, Percent_Identity=25.891181988743, Blast_Score=180, Evalue=2e-45,
Organism=Drosophila melanogaster, GI45551104, Length=376, Percent_Identity=26.063829787234, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI28573474, Length=297, Percent_Identity=26.9360269360269, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR001661
- InterPro:   IPR018232 [H]

Pfam domain/function: PF01204 Trehalase [H]

EC number: =3.2.1.28 [H]

Molecular weight: Translated: 63782; Mature: 63782

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00927 TREHALASE_1 ; PS00928 TREHALASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYL
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCHHHHHH
ELFEHVQSEKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYS
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
SEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFT
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCEEEHHHHH
MLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDV
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
ETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLES
HHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECCEEEEECHHHHHHHHHH
AIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGIYRDYDWRREQLALFSAAAIV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
PLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEECCHHHH
GDDLLGDEIARNWLKTVNQFYLEQHKLIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
RLIGLYGEP
HHHHHCCCC
>Mature Secondary Structure
MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMIEGLPASDALTPADRYL
CCCCCCCCCCCCCEEEEEEEEECCCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCHHHHHH
ELFEHVQSEKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLPEVYS
HHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHH
SEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQSYIVPGGRFSETYYWDSYFT
HHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCHHHCCCCCCCCCCEEEHHHHH
MLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVELFEEDGVR
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCH
GARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDTPRDESWLEDV
HHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCHHHHHHCCCCCCCCCHHHHHHH
ETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDTGRLASIRTTQFIPIDLNAFLFKLES
HHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCEEEEECCEEEEECHHHHHHHHHH
AIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGIYRDYDWRREQLALFSAAAIV
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
PLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPNGWAPLQWMAIQGFKMY
HHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHEECCHHHH
GDDLLGDEIARNWLKTVNQFYLEQHKLIEKYHIADGVPREGGGGEYPLQDGFGWTNGVVR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHH
RLIGLYGEP
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA