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The map label for this gene is rsmD

Identifier: 218691750

GI number: 218691750

Start: 4046516

End: 4047112

Strand: Direct

Name: rsmD

Synonym: ECED1_4138

Alternate gene names: 218691750

Gene position: 4046516-4047112 (Clockwise)

Preceding gene: 218691744

Following gene: 218691751

Centisome position: 77.67

GC content: 54.1

Gene sequence:

>597_bases
ATGAAAAAACCGAATCATTCCGGCAGCGGCCAAATCCGCATTATTGGCGGGCAGTGGCGAGGCCGTAAACTCCCGGTTCC
TGATAGTCCAGGTCTGCGCCCCACCACCGACCGCGTACGCGAAACGTTGTTTAACTGGCTGGCTCCGGTCATTGTTGACG
CCCAATGTCTGGATTGCTTCGCCGGGAGCGGCGCGCTGGGGCTGGAAGCGTTATCGCGCTACGCTGCGGGGGCCACGTTG
ATTGAGATGGATCGTGCGGTTTCTCAGCAGTTAATTAAGAATCTGGCGACACTAAAAGCAGGCAATGCACGCGTGGTGAA
CAGCAACGCGATGTCATTCCTGGCGCAAAAAGGCACACCGCATAATATCGTGTTTGTCGATCCACCGTTCCGCCGTGGCT
TGTTAGAAGAGACGATAAATTTACTGGAAGATAACGGCTGGCTGGCTGACGAAGCCCTGATTTATGTCGAAAGCGAAGTC
GAAAACGGTCTGCCCACTGTTCCGGCAAACTGGTCATTACATCGGGAAAAAGTGGCGGGTCAGGTGGCTTATCGGCTGTA
TCAACGCGAAGCACAAGGAGAAAGTGATGCTGATTAA

Upstream 100 bases:

>100_bases
GTACATAGCAGCTAAAAAAATGATGAAATAGTCTATCACTTAACTTAATTCACATCACCGCCTGCAAGTATGTGTTATCT
GGCGGATTGAGCAATTTATC

Downstream 100 bases:

>100_bases
TATTGGTCGTTTGTTAATGCTCTGTGTTTGGGGATTTTTAATCCTCAACCTGGTGCATCCCTTCCCACGCCCGCTGAATA
TTTTCGTTAACGTGGCGCTG

Product: 16S rRNA m(2)G966-methyltransferase

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATL
IEMDRAVSQQLIKNLATLKAGNARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD

Sequences:

>Translated_198_residues
MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATL
IEMDRAVSQQLIKNLATLKAGNARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD
>Mature_198_residues
MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATL
IEMDRAVSQQLIKNLATLKAGNARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family

Homologues:

Organism=Escherichia coli, GI1789875, Length=198, Percent_Identity=100, Blast_Score=408, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMD_ECOL6 (P0ADY0)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_756120.1
- ProteinModelPortal:   P0ADY0
- SMR:   P0ADY0
- MINT:   MINT-1267276
- EnsemblBacteria:   EBESCT00000041944
- GeneID:   1037074
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c4258
- GeneTree:   EBGT00050000011512
- HOGENOM:   HBG706795
- OMA:   ISGQWRS
- ProtClustDB:   PRK10909
- BRENDA:   2.1.1.52
- InterPro:   IPR002052
- InterPro:   IPR004398
- PIRSF:   PIRSF004553
- TIGRFAMs:   TIGR00095

Pfam domain/function: PF03602 Cons_hypoth95

EC number: =2.1.1.171

Molecular weight: Translated: 21678; Mature: 21678

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVIVDAQCLDCF
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGNARVVNSNAMSFLAQKGTP
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCC
HNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEVENGLPTVPANWSLHREKVAG
CEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHCCCCCCCCCCCCHHHHHHHH
QVAYRLYQREAQGESDAD
HHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLFNWLAPVIVDAQCLDCF
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGNARVVNSNAMSFLAQKGTP
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCC
HNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEVENGLPTVPANWSLHREKVAG
CEEEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECHHCCCCCCCCCCCCHHHHHHHH
QVAYRLYQREAQGESDAD
HHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157