Click here to switch to the map view.

The map label for this gene is aufF [H]

Identifier: 218691708

GI number: 218691708

Start: 3999574

End: 4000368

Strand: Reverse

Name: aufF [H]

Synonym: ECED1_4093

Alternate gene names: 218691708

Gene position: 4000368-3999574 (Counterclockwise)

Preceding gene: 218691709

Following gene: 218691707

Centisome position: 76.79

GC content: 39.25

Gene sequence:

>795_bases
ATGTCCAACCAGGGGATGCCAATGCGTCAGTATCGTTTATTGTCACCTATGATTAAGGTAGTCATAGTATTATTTTCCCT
TTTATTCTCTACAGCACAAGCAGGCTTAGTGATCGGTGGAACGCGTTTTGCGTATCCGGAAAATCAGTCCTCAATATCTG
TTGAACTCAAAAATACTTCAGACAGAGATATGTTAGTCAAGGTTGCAGTCTCCCCTGATGATGCTCGTCAGTTGACGGGA
ACGGTTGAATCTTTGCCCTCATATTCTGAAACGGTATTTATCGCAACTCCGCCACTGTTTGTTTTGAAGCCTGACAAAAA
TACAAAAATAAGAATTAATCGTGTTGGTGGCAGTTTACCTACCGACCGGGAAAGTTTATTTATACTGAATGTTGCAGCAT
TACCAACGTTGGAAAATAGTCATTCAGTCAAAATTGATAATCAATTGCAAATAGCAGTCCGCAATCGGATGAAAATATTC
TACCGGCCATCAAATTTGTCTGAAGACCCGAATATGTCATATCAAAAATTACGCTGGGCACGAAAAAATGAAATGGTTAC
CGTTTATAATCCAGGCCCCAGATATGTGACACTCTATAACCTACATGTTGATGGTAAAGTTATAGATGGTGGTATGGTCG
CACCATTTAGCCATCGCCAACAATCATGGTGTAAATCGCAAGGGGTTTGTGAAATAGCATGGCAAACTTTGGATGTTTAT
AATAATGTATTGCCAATTTTGAAAGTAAAATTAAATCCTTCACAGTTGGGTAATGCTGCCAACTTACTGATTTAG

Upstream 100 bases:

>100_bases
GGTAGTATTAGTAATGATGTTAAAAGCATCGTTTTTTCACTTACACCATCAGTTGATAATATATTGCATTTTTTTGCGGC
TTACAAAGCATTAAAAAATA

Downstream 100 bases:

>100_bases
TGTATGATGGTGATTTTAAGGTGCTTGCGTGGCTTCCATTTCCATCAGATGTCCTTCCTGCTCCGCTACTGAAGGCGTGG
TGCGTAACGGCAAAAGCACT

Product: putative fimbrial chaperone AufF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MSNQGMPMRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSSISVELKNTSDRDMLVKVAVSPDDARQLTG
TVESLPSYSETVFIATPPLFVLKPDKNTKIRINRVGGSLPTDRESLFILNVAALPTLENSHSVKIDNQLQIAVRNRMKIF
YRPSNLSEDPNMSYQKLRWARKNEMVTVYNPGPRYVTLYNLHVDGKVIDGGMVAPFSHRQQSWCKSQGVCEIAWQTLDVY
NNVLPILKVKLNPSQLGNAANLLI

Sequences:

>Translated_264_residues
MSNQGMPMRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSSISVELKNTSDRDMLVKVAVSPDDARQLTG
TVESLPSYSETVFIATPPLFVLKPDKNTKIRINRVGGSLPTDRESLFILNVAALPTLENSHSVKIDNQLQIAVRNRMKIF
YRPSNLSEDPNMSYQKLRWARKNEMVTVYNPGPRYVTLYNLHVDGKVIDGGMVAPFSHRQQSWCKSQGVCEIAWQTLDVY
NNVLPILKVKLNPSQLGNAANLLI
>Mature_263_residues
SNQGMPMRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSSISVELKNTSDRDMLVKVAVSPDDARQLTGT
VESLPSYSETVFIATPPLFVLKPDKNTKIRINRVGGSLPTDRESLFILNVAALPTLENSHSVKIDNQLQIAVRNRMKIFY
RPSNLSEDPNMSYQKLRWARKNEMVTVYNPGPRYVTLYNLHVDGKVIDGGMVAPFSHRQQSWCKSQGVCEIAWQTLDVYN
NVLPILKVKLNPSQLGNAANLLI

Specific function: Could be required for the biogenesis of a putative fimbria [H]

COG id: COG3121

COG function: function code NU; P pilus assembly protein, chaperone PapD

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Ig-like (immunoglobulin-like) domain [H]

Homologues:

Organism=Escherichia coli, GI87081806, Length=227, Percent_Identity=35.2422907488987, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1786743, Length=237, Percent_Identity=32.0675105485232, Blast_Score=122, Evalue=3e-29,
Organism=Escherichia coli, GI1787171, Length=235, Percent_Identity=31.9148936170213, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790771, Length=248, Percent_Identity=29.0322580645161, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1786333, Length=207, Percent_Identity=33.3333333333333, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789532, Length=229, Percent_Identity=29.6943231441048, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1788428, Length=220, Percent_Identity=29.0909090909091, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1786936, Length=185, Percent_Identity=28.1081081081081, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082203, Length=179, Percent_Identity=28.4916201117318, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008962
- InterPro:   IPR001829
- InterPro:   IPR016148
- InterPro:   IPR018046
- InterPro:   IPR016147 [H]

Pfam domain/function: PF02753 Pili_assembly_C; PF00345 Pili_assembly_N [H]

EC number: NA

Molecular weight: Translated: 29583; Mature: 29451

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQGMPMRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSSISVELKNTS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEECCCCCCEEEEEEECCC
DRDMLVKVAVSPDDARQLTGTVESLPSYSETVFIATPPLFVLKPDKNTKIRINRVGGSLP
CCEEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCEEEEEECCCCCC
TDRESLFILNVAALPTLENSHSVKIDNQLQIAVRNRMKIFYRPSNLSEDPNMSYQKLRWA
CCCCCEEEEEEEECCCCCCCCEEEECCEEEEEEECCEEEEECCCCCCCCCCCCHHHHHHH
RKNEMVTVYNPGPRYVTLYNLHVDGKVIDGGMVAPFSHRQQSWCKSQGVCEIAWQTLDVY
CCCCEEEEECCCCCEEEEEEEEECCEEECCCEECCCCHHHHHHHHHCCEEEEHHHHHHHH
NNVLPILKVKLNPSQLGNAANLLI
HCCEEEEEEEECHHHCCCCCCCCC
>Mature Secondary Structure 
SNQGMPMRQYRLLSPMIKVVIVLFSLLFSTAQAGLVIGGTRFAYPENQSSISVELKNTS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEECCCCCCEEEEEEECCC
DRDMLVKVAVSPDDARQLTGTVESLPSYSETVFIATPPLFVLKPDKNTKIRINRVGGSLP
CCEEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCEEEEEECCCCCC
TDRESLFILNVAALPTLENSHSVKIDNQLQIAVRNRMKIFYRPSNLSEDPNMSYQKLRWA
CCCCCEEEEEEEECCCCCCCCEEEECCEEEEEEECCEEEEECCCCCCCCCCCCHHHHHHH
RKNEMVTVYNPGPRYVTLYNLHVDGKVIDGGMVAPFSHRQQSWCKSQGVCEIAWQTLDVY
CCCCEEEEECCCCCEEEEEEEEECCEEECCCEECCCCHHHHHHHHHCCEEEEHHHHHHHH
NNVLPILKVKLNPSQLGNAANLLI
HCCEEEEEEEECHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 2992959; 7984428 [H]