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The map label for this gene is greB

Identifier: 218691683

GI number: 218691683

Start: 3968898

End: 3969374

Strand: Direct

Name: greB

Synonym: ECED1_4066

Alternate gene names: 218691683

Gene position: 3968898-3969374 (Clockwise)

Preceding gene: 218691680

Following gene: 218691684

Centisome position: 76.19

GC content: 49.9

Gene sequence:

>477_bases
ATGAAAACGCCCCTGGTTACCCGGGAAGGGTATGAAAAACTCAAACAAGAGCTTAATTATCTCTGGCGTGAAGAACGCCC
GGAGGTCACAAAGAAAGTGACCTGGGCCGCAAGTCTGGGCGACCGCAGCGAAAATGCTGACTATCAGTATAATAAAAAGC
GCCTGCGTGAAATCGACCGTCGCGTGCGCTATCTCACTAAATGCATGGAAAATCTCAAAATCGTCGATTACTCCCCGCAG
CAGGAAGGCAAAGTCTTTTTTGGCGCGTGGGTGGAGATTGAAAACGACGATGGTGTGACTCACCGTTTCCGTATTGTCGG
CTACGATGAAATTTTTGGCCGTAAAGATTACATCTCTATCGATTCCCCGATGGCCCGCGCATTGCTGAAAAAAGAAGTCG
GCGATCTGGCGGTGGTGAATACCCCTGCCGGGGAAGCGAGCTGGTATGTTAATGCTATCGAGTACGTGAAACCGTAA

Upstream 100 bases:

>100_bases
TAAGTATACAATTTATTCGGCGAAACATTATTGATTCTGTTGATATGATCACGTTATACCCAATGTGCGCATTATCAAAC
AGACAAAGGGAATCAACGAG

Downstream 100 bases:

>100_bases
GGAAGAGTCTTAACCTCCTGCCGATGGCTGGCATTTTTGCCAGCCAGTCCGTATAACTATCCCCTGATTTTTGATCCGAA
AAGATGAACTCAAACCATGA

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQ
QEGKVFFGAWVEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP

Sequences:

>Translated_158_residues
MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQ
QEGKVFFGAWVEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP
>Mature_158_residues
MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQ
QEGKVFFGAWVEIENDDGVTHRFRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082261, Length=158, Percent_Identity=99.3670886075949, Blast_Score=324, Evalue=2e-90,
Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=34.1772151898734, Blast_Score=97, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GREB_ECO57 (P64274)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   G86005
- PIR:   H91159
- RefSeq:   NP_289946.2
- RefSeq:   NP_312275.2
- ProteinModelPortal:   P64274
- SMR:   P64274
- EnsemblBacteria:   EBESCT00000029072
- EnsemblBacteria:   EBESCT00000056122
- GeneID:   915895
- GeneID:   958110
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4761
- KEGG:   ecs:ECs4248
- GeneTree:   EBGT00050000010088
- HOGENOM:   HBG753387
- OMA:   KRFRIVG
- ProtClustDB:   PRK01885
- BioCyc:   ECOL83334:ECS4248-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01461

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 18545; Mature: 18545

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDR
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHEEHHHHCCCCCCCCCCHHHHHHHHHHHH
RVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHRFRIVGYDEIFGRKDYISI
HHHHHHHHHHCCEEEECCCCCCCCEEEEEEEEEECCCCCEEEEEEEEHHHHHCCCCEEEC
DSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP
CCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSENADYQYNKKRLREIDR
CCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHEEHHHHCCCCCCCCCCHHHHHHHHHHHH
RVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHRFRIVGYDEIFGRKDYISI
HHHHHHHHHHCCEEEECCCCCCCCEEEEEEEEEECCCCCEEEEEEEEHHHHHCCCCEEEC
DSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP
CCHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796