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The map label for this gene is yrfG

Identifier: 218691675

GI number: 218691675

Start: 3960754

End: 3961422

Strand: Direct

Name: yrfG

Synonym: ECED1_4058

Alternate gene names: 218691675

Gene position: 3960754-3961422 (Clockwise)

Preceding gene: 218691674

Following gene: 218691676

Centisome position: 76.03

GC content: 51.12

Gene sequence:

>669_bases
ATGCATATCAACATTGCCTGGCAGGACGTAGATACCGTTCTGCTGGATATGGACGGCACGTTGCTCGACCTCGCCTTCGA
TAACTATTTCTGGCAAAAGCTGGTGCCTGAAACATGGGGCGCGAAAAACGGAGTTACGCCACAGGAAGCGATGGAATATA
TGCGCCAGCAATATCACGACGTACAGCATACGCTAAACTGGTACTGTCTTGATTACTGGAGTGAGCAACTGGGTCTGGAT
ATCTGTGCGATGACCACCGAGATGGGACCGCGTGCCGTACTGCGTGAAGATACCATTCCGTTTCTTGAGGCACTGAAAGC
CAGCGGTAAACAGCGAATTTTGCTCACCAATGCGCATCCGCACAACCTGGCGGTAAAACTTGAGCATACCGGTCTGGACG
CACACCTTGATTTATTACTTTCCACCCACACATTTGGTTATCCGAAAGAGGATCAGCGGTTATGGCATGCGGTGGCCGAA
GCTACGGGTCTTAAAGCTGAAAGAACGCTGTTTATTGATGACAGCGAAGCGATTCTCGATGCTGCCGCGCAATTTGGTAT
TCGTTACTGCCTCGGTGTGACTAATCCTGACTCCGGGATTGCCGAGAAACAGTATCAACGCCATCCGTCACTGAATGACT
ACCGCCGCCTGATCCCCTCGCTAATGTGA

Upstream 100 bases:

>100_bases
CAACTGATCACCCCTTCAGAAAGCCTTATCGAATAACACGTTTGCGCGGCAGGTTATGCTACCCTGTCGCGCAAATTGCT
TCACTCTGGAGATTTCCCTC

Downstream 100 bases:

>100_bases
AGGAGATGCCATGAAAGAAAAACCTGCTGTTGAGGTTCGACTGGATAAATGGCTATGGGCTGCCCGTTTTTATAAAACCC
GCGCGCTGGCCCGTGAAATG

Product: putative hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNGVTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLD
ICAMTTEMGPRAVLREDTIPFLEALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLIPSLM

Sequences:

>Translated_222_residues
MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNGVTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLD
ICAMTTEMGPRAVLREDTIPFLEALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLIPSLM
>Mature_222_residues
MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNGVTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLD
ICAMTTEMGPRAVLREDTIPFLEALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLIPSLM

Specific function: Unknown

COG id: COG1011

COG function: function code R; Predicted hydrolase (HAD superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082259, Length=222, Percent_Identity=100, Blast_Score=464, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YRFG_ECOLI (P64636)

Other databases:

- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B65135
- RefSeq:   AP_004391.1
- RefSeq:   NP_417858.4
- ProteinModelPortal:   P64636
- SMR:   P64636
- STRING:   P64636
- EnsemblBacteria:   EBESCT00000002012
- EnsemblBacteria:   EBESCT00000017090
- GeneID:   947904
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW5865
- KEGG:   eco:b3399
- EchoBASE:   EB2764
- EcoGene:   EG12928
- eggNOG:   COG1011
- GeneTree:   EBGT00050000010692
- HOGENOM:   HBG298752
- OMA:   LVTNAHP
- ProtClustDB:   PRK14988
- BioCyc:   EcoCyc:G7742-MONOMER
- BioCyc:   MetaCyc:G7742-MONOMER
- Genevestigator:   P64636
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006402
- InterPro:   IPR005833
- Gene3D:   G3DSA:3.40.50.1000
- PRINTS:   PR00413
- TIGRFAMs:   TIGR01509

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: NA

Molecular weight: Translated: 25399; Mature: 25399

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNGVTPQEAMEYMRQQYHD
CEEEEEECCCCEEEEECCCCEEEHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHH
VQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFLEALKASGKQRILLTNAHP
HHHHHHHHHHHHHHHHHCCCCEEHHHCCCCHHHHHCCCHHHHHHHHCCCCCEEEEECCCC
HNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAEATGLKAERTLFIDDSEAILD
CCEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHH
AAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLIPSLM
HHHHHHHHHEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure
MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNGVTPQEAMEYMRQQYHD
CEEEEEECCCCEEEEECCCCEEEHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHH
VQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFLEALKASGKQRILLTNAHP
HHHHHHHHHHHHHHHHHCCCCEEHHHCCCCHHHHHCCCHHHHHHHHCCCCCEEEEECCCC
HNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAEATGLKAERTLFIDDSEAILD
CCEEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCHHHHHH
AAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLIPSLM
HHHHHHHHHEEECCCCCCCHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503