Click here to switch to the map view.

The map label for this gene is adh1 [H]

Identifier: 218691107

GI number: 218691107

Start: 3390999

End: 3392117

Strand: Direct

Name: adh1 [H]

Synonym: ECED1_3448

Alternate gene names: 218691107

Gene position: 3390999-3392117 (Clockwise)

Preceding gene: 218691104

Following gene: 218691108

Centisome position: 65.09

GC content: 52.46

Gene sequence:

>1119_bases
ATGGCCCGCTATTATTTTTCGTGCCCCAAAATTTATTTTGGTGAAGAGGCAATAACCTCTCTATGCCAATTTAATAATAA
ACGAGTCGGTATTGTCACCGATGATTTTATGATGTCATCAGGTAAAACTCGTTATTTAATAAGTCATATGCCTGAGGCAT
CGGTATGCATTTTCGGTGAGGTTACTCCGGATCCAGATATTGATATTCTGACTTCCGGCGCGGCGCGTTTTAAAGCCTTT
AAGCCCGACGTTATTATCGCCCTCGGCGGCGGCTCGTCGCTGGACGCTGCGAAAGGAATTAAAGTCACATTGGAAGAGTA
TTTTGGAGATCATCAAATCGATCTCATTGCTATTCCCACAACCAGCGGATCCGGCTCTGAAGTGACGTCCTACGCGATCA
TCTCTGATCCGCAAAATGGCCGCAAATATCCGTTGATCTCCGGGCAACTGGTGCCGGAAATCGCCATTCTCGATCCTAAC
CTAGTGTTAACCGCGCCGCGTCATGTCGCGGTAGATACCGGCATGGACGTGCTGACCCATGCCATTGAAGCCTTTGTCTC
TACTGGCTCAAATGACTTTAGCGATGCGCTGGCGGAAAAAGCCATCGCGTTGACCTGGCGCTATCTGCCGCAGGTGTTTA
ACGACGAAAAAGACATTGCGGCGCGCACCCACATGCACAATGCCTCCTGTATGGCAGGCATGGCATTTAACTCCGCCGGG
CTGGGGTTGGTACACGGCATGGCACACGCCATCGGCGGGATGCTGCATATTCCTCATGGCAAAATTAACGCCATGCTGCT
GCCGATTGTCATCGACTTCAACAGCAAACGGGCCAGCACTGAAACCCGCGATCGCTATCACCGTTGCGCGCAAATCATAG
GCATTGACCATGCGGTACCGGAGCAGGCCATCGCGCTGATGACTCAGGAGATCCGCCAGATCAATCGCCATTTCTGCATC
CCCGTCACTCTCAGCGAACTGGGCATCGACCGTGCAAAAATCATCGAGTTGCGCAGCGCACTGGTGAATTCGACGCTGGC
CGACGGTTGTACCGCGTCGAACCCGCGGCAGGTGACGACGCATGATGTCGAAGGGTTGATCGACCTGATTACCGGCTAG

Upstream 100 bases:

>100_bases
ATCCTTCACCTGCACAAACATCCTCAGTCCATTAAAAAAATCGCCATGTTAGGTTAACGACATCAAATTTGGTCGTTTAC
CATGATTAAGGTAGTTACAC

Downstream 100 bases:

>100_bases
CTGGATAACTTTGTTCAGCCTCCGTGAAGCAGTCAAAAAAGTCCTGCTGATAAACAAAAACCGGGCGTCGTTACTCCCTA
TCCTGATCGTTAGACGCCCA

Product: putative propanol dehydrogenase

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MARYYFSCPKIYFGEEAITSLCQFNNKRVGIVTDDFMMSSGKTRYLISHMPEASVCIFGEVTPDPDIDILTSGAARFKAF
KPDVIIALGGGSSLDAAKGIKVTLEEYFGDHQIDLIAIPTTSGSGSEVTSYAIISDPQNGRKYPLISGQLVPEIAILDPN
LVLTAPRHVAVDTGMDVLTHAIEAFVSTGSNDFSDALAEKAIALTWRYLPQVFNDEKDIAARTHMHNASCMAGMAFNSAG
LGLVHGMAHAIGGMLHIPHGKINAMLLPIVIDFNSKRASTETRDRYHRCAQIIGIDHAVPEQAIALMTQEIRQINRHFCI
PVTLSELGIDRAKIIELRSALVNSTLADGCTASNPRQVTTHDVEGLIDLITG

Sequences:

>Translated_372_residues
MARYYFSCPKIYFGEEAITSLCQFNNKRVGIVTDDFMMSSGKTRYLISHMPEASVCIFGEVTPDPDIDILTSGAARFKAF
KPDVIIALGGGSSLDAAKGIKVTLEEYFGDHQIDLIAIPTTSGSGSEVTSYAIISDPQNGRKYPLISGQLVPEIAILDPN
LVLTAPRHVAVDTGMDVLTHAIEAFVSTGSNDFSDALAEKAIALTWRYLPQVFNDEKDIAARTHMHNASCMAGMAFNSAG
LGLVHGMAHAIGGMLHIPHGKINAMLLPIVIDFNSKRASTETRDRYHRCAQIIGIDHAVPEQAIALMTQEIRQINRHFCI
PVTLSELGIDRAKIIELRSALVNSTLADGCTASNPRQVTTHDVEGLIDLITG
>Mature_371_residues
ARYYFSCPKIYFGEEAITSLCQFNNKRVGIVTDDFMMSSGKTRYLISHMPEASVCIFGEVTPDPDIDILTSGAARFKAFK
PDVIIALGGGSSLDAAKGIKVTLEEYFGDHQIDLIAIPTTSGSGSEVTSYAIISDPQNGRKYPLISGQLVPEIAILDPNL
VLTAPRHVAVDTGMDVLTHAIEAFVSTGSNDFSDALAEKAIALTWRYLPQVFNDEKDIAARTHMHNASCMAGMAFNSAGL
GLVHGMAHAIGGMLHIPHGKINAMLLPIVIDFNSKRASTETRDRYHRCAQIIGIDHAVPEQAIALMTQEIRQINRHFCIP
VTLSELGIDRAKIIELRSALVNSTLADGCTASNPRQVTTHDVEGLIDLITG

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=316, Percent_Identity=25.6329113924051, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1787493, Length=395, Percent_Identity=34.9367088607595, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87082107, Length=379, Percent_Identity=35.6200527704486, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI48994951, Length=363, Percent_Identity=33.3333333333333, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI1789163, Length=374, Percent_Identity=32.0855614973262, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1789386, Length=364, Percent_Identity=23.3516483516484, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17537053, Length=340, Percent_Identity=28.5294117647059, Blast_Score=120, Evalue=9e-28,
Organism=Saccharomyces cerevisiae, GI6321181, Length=381, Percent_Identity=33.8582677165354, Blast_Score=195, Evalue=9e-51,
Organism=Drosophila melanogaster, GI24657991, Length=346, Percent_Identity=29.7687861271676, Blast_Score=93, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 40297; Mature: 40165

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYYFSCPKIYFGEEAITSLCQFNNKRVGIVTDDFMMSSGKTRYLISHMPEASVCIFGE
CCCEEECCCCEEECHHHHHHHHHCCCCEEEEEEHHHHHCCCCEEEEEECCCCCCEEEEEC
VTPDPDIDILTSGAARFKAFKPDVIIALGGGSSLDAAKGIKVTLEEYFGDHQIDLIAIPT
CCCCCCCEEEECCCHHHEECCCCEEEEECCCCCCCCCCCCEEEHHHHCCCCCEEEEEEEC
TSGSGSEVTSYAIISDPQNGRKYPLISGQLVPEIAILDPNLVLTAPRHVAVDTGMDVLTH
CCCCCCCEEEEEEEECCCCCCCCCEECCCCCCEEEEECCCEEEECCCEEEECCCHHHHHH
AIEAFVSTGSNDFSDALAEKAIALTWRYLPQVFNDEKDIAARTHMHNASCMAGMAFNSAG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHCCEEECCCC
LGLVHGMAHAIGGMLHIPHGKINAMLLPIVIDFNSKRASTETRDRYHRCAQIIGIDHAVP
HHHHHHHHHHHCCEEECCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCH
EQAIALMTQEIRQINRHFCIPVTLSELGIDRAKIIELRSALVNSTLADGCTASNPRQVTT
HHHHHHHHHHHHHHHCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEH
HDVEGLIDLITG
HHHHHHHHHHCC
>Mature Secondary Structure 
ARYYFSCPKIYFGEEAITSLCQFNNKRVGIVTDDFMMSSGKTRYLISHMPEASVCIFGE
CCEEECCCCEEECHHHHHHHHHCCCCEEEEEEHHHHHCCCCEEEEEECCCCCCEEEEEC
VTPDPDIDILTSGAARFKAFKPDVIIALGGGSSLDAAKGIKVTLEEYFGDHQIDLIAIPT
CCCCCCCEEEECCCHHHEECCCCEEEEECCCCCCCCCCCCEEEHHHHCCCCCEEEEEEEC
TSGSGSEVTSYAIISDPQNGRKYPLISGQLVPEIAILDPNLVLTAPRHVAVDTGMDVLTH
CCCCCCCEEEEEEEECCCCCCCCCEECCCCCCEEEEECCCEEEECCCEEEECCCHHHHHH
AIEAFVSTGSNDFSDALAEKAIALTWRYLPQVFNDEKDIAARTHMHNASCMAGMAFNSAG
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHCCEEECCCC
LGLVHGMAHAIGGMLHIPHGKINAMLLPIVIDFNSKRASTETRDRYHRCAQIIGIDHAVP
HHHHHHHHHHHCCEEECCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCH
EQAIALMTQEIRQINRHFCIPVTLSELGIDRAKIIELRSALVNSTLADGCTASNPRQVTT
HHHHHHHHHHHHHHHCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEH
HDVEGLIDLITG
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]